Essential Uncharacterized ORFs - Yeast Resource Center

ORF

GO Terms Assignment

Experimental and Computational Evidence

Comments

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	None

YDL193W

Bio. Proc.:	Unknown
Cell. Comp.:	Nuclear membrane, lipid particle
Mol. Func.:	Prenyltransferase

Mass Spec.:	No data
Localization:	Nuclear membrane, lipid particle
Two-Hybrid:	No data
Struc. Pred.:	Undecaprenyl diphosphate synthase

None

YDL196W

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	Membrane all- alpha superfamily

YDL209C (CWC2)

Bio. Proc.:	mRNA splicing
Cell. Comp.:	Nucleus
Mol. Func.:	RNA- binding

Mass Spec.:	mRNA splicing
Localization:	Nucleus
Two-Hybrid:	None*
Struc. Pred.:	Two RNA- binding domains

None

YDR013W (PSF1)

Bio. Proc.:	DNA repair
Cell. Comp.:	Nucleus ¹
Mol. Func.:	Unknown

Mass Spec.:	Unknown complex 1
Localization:	No data
Two-Hybrid:	DNA repair *
Struc. Pred.:	None

Copurification with TAP- tagged YJL072c and two- hybrid analysis allowed placement into unknown complex 1. The two- hybrid analysis also detected interaction with proteins involved in DNA repair. This allowed annotation of the entire complex.

YDR196C

Bio. Proc.:	Unknown
Cell. Comp.:	Nuclear envelope- endoplasmic reticulum network
Mol. Func.:	Dephospho- CoA kinase

Mass Spec.:	None
Localization:	Nuclear envelope- endoplasmic reticulum network
Two-Hybrid:	None
Struc. Pred.:	Dephospho- CoA kinase

None

YDR267C

Bio. Proc.:	Unknown
Cell. Comp.:	Cytoplasm, nucleus
Mol. Func.:	Transcription regulator ³

Mass Spec.:	None
Localization:	Cytoplasm, nucleus
Two-Hybrid:	None
Struc. Pred.:	Tup1, C- terminal domain; WD40 repeat superfamily

Function was predicted from the multiple two- hybrid interactions with transcription factors.

YDR288W

Bio. Proc.:	DNA repair
Cell. Comp.:	Nucleus
Mol. Func.:	DNA binding

Mass Spec.:	DNA repair
Localization:	Nucleus
Two-Hybrid:	Response to DNA damage stimulus*
Struc. Pred.:	Adenylation domain of NAD+- dependent DNA ligase; Winged helix DNA- binding domain

None

YDR339C

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No positives
Struc. Pred.:	None

YDR365C

Bio. Proc.:	rRNA processing
Cell. Comp.:	Nucleolus
Mol. Func.:	Unknown

Mass Spec.:	rRNA processing
Localization:	Nucleolus
Two-Hybrid:	None
Struc. Pred.:	Unknown

None

YDR367W

Bio. Proc.:	Unknown
Cell. Comp.:	Golgi apparatus
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Golgi apparatus
Two-Hybrid:	None
Struc. Pred.:	Membrane all- alpha superfamily

Two- hybrid detected a single interaction with RGD2, for which the biological process is small GTPase mediated signal transduction.

YDR396W

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	None

YDR413C

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	None
Struc. Pred.:	Membrane all- alpha superfamily

None

YDR437W

Bio. Proc.:	Unknown
Cell. Comp.:	Nuclear envelope- endoplasmic reticulum network
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Nuclear envelope- endoplasmic reticulum network
Two-Hybrid:	None
Struc. Pred.:	Bacterial enterotoxin and exotoxin superfamily

Two- hybrid detects interactions with membrane proteins annotated as involved in detoxification by MIPS

YDR489W (SLD5)

Bio. Proc.:	DNA repair
Cell. Comp.:	Nucleus ¹
Mol. Func.:	Unknown

Mass Spec.:	Unknown complex 1
Localization:	No data
Two-Hybrid:	Unknown complex 1^*
Struc. Pred.:	Interferon- induced guanylate- binding protein

Copurification with TAP- tagged YJL072c and two- hybrid analysis allowed placement into unknown complex 1.

YDR527W

Bio. Proc.:	Unknown
Cell. Comp.:	Cytoplasm
Mol. Func.:	Unknown

Mass Spec.:	None
Localization:	Cytoplasm
Two-Hybrid:	No positives
Struc. Pred.:	ARM repeat superfamily

None

YDR531W

Bio. Proc.:	Coenzyme A biosynthesis ²
Cell. Comp.:	Cytoplasm, nucleus
Mol. Func.:	Pantothenate kinase

Mass Spec.:	None
Localization:	Cytoplasm, nucleus
Two-Hybrid:	None
Struc. Pred.:	Fumble, similar to pantothenate kinases

Protein structure prediction provided process annotation.

YFR003C

Bio. Proc.:	Cell cycle
Cell. Comp.:	Cytoplasm, nucleus ¹
Mol. Func.:	Protein phosphatase inhibitor

Mass Spec.:	Cell cycle
Localization:	No signal
Two-Hybrid:	Morphogenesis *
Struc. Pred.:	Protein phosphatase type 1 inhibitor (PSI)

Glc7 and SDS22 were identified by co- purification and two- hybrid and are the basis of the cell cycle process annotation. Morphogenesis was the prediction by two- hybrid due to the detection of SIP2 which along with Glc7 has a morphogenesis annotation

YFR042W

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Unknown
Two-Hybrid:	None
Struc. Pred.:	None

YGL047W

Bio. Proc.:	Unknown
Cell. Comp.:	Cytoplasm, nucleus
Mol. Func.:	Transferase, transferring hexosyl groups

Mass Spec.:	None
Localization:	Cytoplasm, nucleus
Two-Hybrid:	No positives
Struc. Pred.:	UDP- Glycosyltransferase/ glycogen phosphorylase superfamily

None

YGL098W (USE1)

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	Colicin E3 immunity protein

YGR002C

Bio. Proc.:	Histone acetylation
Cell. Comp.:	Nucleus
Mol. Func.:	DNA binding

Mass Spec.:	Histone acetylation
Localization:	Nucleus
Two-Hybrid:	Nuclear division *
Struc. Pred.:	Histone methyltransferase associated binding protein (PSI); Myb- like DNA- binding domain protein family

None

YGR024C

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	None
Struc. Pred.:	None

YGR046W

Bio. Proc.:	Mitochondrial translocation
Cell. Comp.:	Mitochondrion
Mol. Func.:	Unknown

Mass Spec.:	Mitochondrial translocation
Localization:	Mitochondrion
Two-Hybrid:	None
Struc. Pred.:	None

YGR046w is in a stoichiometric complex with Hsp60

YGR145W

Bio. Proc.:	rRNA processing
Cell. Comp.:	Nucleolus
Mol. Func.:	Unknown

Mass Spec.:	rRNA processing
Localization:	Nucleolus
Two-Hybrid:	No data
Struc. Pred.:	WD40 repeat superfamily

YGR145w copurified with TAP tagged YDR365c.

YGR198W

Bio. Proc.:	MAPKKK cascade
Cell. Comp.:	Plasma membrane
Mol. Func.:	Transferase

Mass Spec.:	MAPKKK cascade
Localization:	Plasma membrane
Two-Hybrid:	None
Struc. Pred.:	N- acetylglucosamine transferase (PSI)

Copurifies with Stt4 which functions in the PKC1 protein kinase pathway

YGR251W

Bio. Proc.:	Unknown
Cell. Comp.:	Nucleolus, nucleus
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Nucleolus, nucleus
Two-Hybrid:	No data
Struc. Pred.:	None

None

YGR277C

Bio. Proc.:	Unknown
Cell. Comp.:	Cytoplasm, nucleus
Mol. Func.:	Nucleotidyl- transferase

Mass Spec.:	None
Localization:	Cytoplasm, nucleus
Two-Hybrid:	None
Struc. Pred.:	Nucleotide- diphospho- sugar transferases superfamily; Cytidylyltransferase family

None

YHR036W

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	Hepatitis B viral capsid (hbcag) superfamily

YHR040W (BCD1)

Bio. Proc.:	Unknown
Cell. Comp.:	Nucleus
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Nucleus
Two-Hybrid:	None
Struc. Pred.:	None

None

YHR083W

Bio. Proc.:	Unknown
Cell. Comp.:	Mitochondrion
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Mitochondrion
Two-Hybrid:	None
Struc. Pred.:	None

None

YHR085W

Bio. Proc.:	Unknown
Cell. Comp.:	Nucleus
Mol. Func.:	Unknown

Mass Spec.:	Unknown complex 2
Localization:	Nucleus
Two-Hybrid:	None
Struc. Pred.:	None

Two hybrid prediction from two positives suggests a process of cell cycle but did not use this since there was no other supporting interactions from the other members of this complex.

YHR122W

Bio. Proc.:	Transcription
Cell. Comp.:	Cytoplasm, nucleus
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Cytoplasm, nucleus
Two-Hybrid:	Transcription
Struc. Pred.:	None

YHR122w and YDR267c may be involved in a similar process because both interact with multiple transcription factors in two- hybrid screen.

YHR197W (RIX1)

Bio. Proc.:	Unknown
Cell. Comp.:	Nucleus ¹
Mol. Func.:	Unknown

Mass Spec.:	Unknown complex 2
Localization:	No signal
Two-Hybrid:	Unknown complex 2^*
Struc. Pred.:	None

None

YIL019W

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	None

YIL083C

Bio. Proc.:	Coenzyme A biosynthesis
Cell. Comp.:	Cytoplasm, nucleus
Mol. Func.:	Phospho- pantothenoyl- cysteine ligase

Mass Spec.:	None
Localization:	Cytoplasm, nucleus
Two-Hybrid:	No positives
Struc. Pred.:	Phospho- pantothenoyl- cysteine synthetase/ decarboxylase (PSI); Pantothenate metabolism flavoprotein superfamily

Remote homology searches and protein strucutre prediction provided process annotation.

YIL091C

Bio. Proc.:	Unknown
Cell. Comp.:	Nucleolus, nucleus
Mol. Func.:	RNA helicase

Mass Spec.:	None
Localization:	Nucleolus, nucleus
Two-Hybrid:	None
Struc. Pred.:	DEAD box RNA helicase

None

YIR010W (DSN1)

Bio. Proc.:	Chromosome segregation
Cell. Comp.:	Kinetochore
Mol. Func.:	Unknown

Mass Spec.:	Chromosome segregation
Localization:	Kinetochore
Two-Hybrid:	None*
Struc. Pred.:	Myosin motor domain

Two- hybrid has one positive which overlaps with the co- purification results.

YJL010C

Bio. Proc.:	rRNA processing
Cell. Comp.:	Nucleolus, nucleus
Mol. Func.:	RNA binding

Mass Spec.:	rRNA processing
Localization:	Nucleolus, nucleus
Two-Hybrid:	None
Struc. Pred.:	Pumilio family RNA binding

None

YJL072C (PSF2)

Bio. Proc.:	DNA repair
Cell. Comp.:	Nucleus
Mol. Func.:	Unknown

Mass Spec.:	Unknown complex 1
Localization:	Nucleus
Two-Hybrid:	Unknown complex 1^*
Struc. Pred.:	ARM repeat, Cytokines superfamily

TAP- tagged YJL072C purified the other three members of the complex in stoichiometric amounts.

YJL091C (GWT1)

Bio. Proc.:	Secretory pathway
Cell. Comp.:	Nuclear envelope- endoplasmic reticulum network
Mol. Func.:	Unknown

Mass Spec.:	Secretory pathway
Localization:	Nuclear envelope- endoplasmic reticulum network
Two-Hybrid:	No data
Struc. Pred.:	4- helical cytokines superfamily

None

YJL097W

Bio. Proc.:	Nuclear membrane fusion
Cell. Comp.:	Nuclear envelope- endoplasmic reticulum network, vacuolar membrane, vacuole
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Nuclear envelope- endoplasmic reticulum network, vacuolar membrane, vacuole
Two-Hybrid:	Nuclear membrane fusion
Struc. Pred.:	Protein tyrosine phosphatase like (PSI); Membrane all- alpha superfamily

Two- hybrid analysis indicates 9/ 11 interactions involve membrane proteins. Nuclear membrane fusion is the predicted GO process because most interacting proteins have unknown function and processes.

YJR012C

Bio. Proc.:	Transport
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	Transport
Localization:	No signal
Two-Hybrid:	No positives
Struc. Pred.:	None

None

YJR013W

Bio. Proc.:	Amino acid transport
Cell. Comp.:	Nuclear envelope- endoplasmic reticulum network
Mol. Func.:	Mannosyl transferase

Mass Spec.:	None
Localization:	Nuclear envelope- endoplasmic reticulum network
Two-Hybrid:	Amino acid transport
Struc. Pred.:	Mannosyl transferase (PSI)

Two- hybrid analysis detected interactions with 18 membrane proteins, 4 of which are involved in amino acid transport. The variety of membrane protein classes and the mannosyltransferase function suggest a membrane protein processing role.

YJR023C

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	None
Struc. Pred.:	None

YJR041C

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	ARM repeat superfamily

YJR072C

Bio. Proc.:	Aerobic respiration
Cell. Comp.:	Cytoplasm
Mol. Func.:	Signal peptide binding

Mass Spec.:	None
Localization:	Cytoplasm
Two-Hybrid:	Aerobic respiration
Struc. Pred.:	Signal peptide- binding domain superfamily; GTPase domain of the signal sequence recognition protein Ffh

Process prediction is based on interactions with inner mitochondrial proteins involved in aerobic respiration. Signal sequence binding and unusual cytoplamic localization suggest an alternative process term would be protein targeting. Interaction was also detected withYOR262w which has a similar structural prediction of a domain of the signal recognition particle.

YJR136C

Bio. Proc.:	Protein biosynthesis
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	Protein biosynthesis
Localization:	No signal
Two-Hybrid:	No data
Struc. Pred.:	None

Process prediction based on copurification with mitochondrial small ribosomal subunit, Rsm23.

YJR141W

Bio. Proc.:	mRNA processing
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No signal
Two-Hybrid:	mRNA processing
Struc. Pred.:	None

None

YKL014C

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	None
Localization:	No data
Two-Hybrid:	None
Struc. Pred.:	ARM repeat superfamily

YKL033W

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	ARM repeat superfamily

YKL082C

Bio. Proc.:	Establishment of cell polarity
Cell. Comp.:	Nucleolus, nucleus
Mol. Func.:	Structural molecule

Mass Spec.:	No data
Localization:	Nucleolus, nucleus
Two-Hybrid:	Establishment of cell polarity
Struc. Pred.:	Nucleolar matrix protein SURF- 6 (PSI)

None

YKL088W

Bio. Proc.:	Coenzyme A biosynthesis ²
Cell. Comp.:	Cytoplasm
Mol. Func.:	Phospho- pantothenoyl- cysteine decarboxylase

Mass Spec.:	Coenzyme A biosyntehsis
Localization:	Cytoplasm
Two-Hybrid:	Cellular process
Struc. Pred.:	Phospho- pantothenoyl- cysteine decarboxylase; Pantothenate metabolism flavoprotein superfamily

None

YKL195W

Bio. Proc.:	Protein targeting
Cell. Comp.:	Mitochondrion
Mol. Func.:	Unknown

Mass Spec.:	Protein targeting
Localization:	Mitochondrion
Two-Hybrid:	No positives
Struc. Pred.:	None

None

YKR022C

Bio. Proc.:	mRNA splicing
Cell. Comp.:	Nucleus
Mol. Func.:	Unknown

Mass Spec.:	mRNA splicing
Localization:	Nucleus
Two-Hybrid:	None*
Struc. Pred.:	None

Interaction between YLR424w and YKR022c was detected by both two- hybrid and mass spectrometry.

YKR038C

Bio. Proc.:	Response to dessication
Cell. Comp.:	Nucleus
Mol. Func.:	ATPase

Mass Spec.:	Response to dessication
Localization:	Nucleus
Two-Hybrid:	No data
Struc. Pred.:	Glycoprotein endopeptidase (PSI); Actin- like ATPase domain

None

YKR079C

Bio. Proc.:	DNA catabolism; RNA catabolism
Cell. Comp.:	Nucleus, Mitochondria
Mol. Func.:	Nuclease

Mass Spec.:	DNA or RNA catabolism
Localization:	Nucleus, Mitochondrion
Two-Hybrid:	No positives
Struc. Pred.:	tRNA endoribonuclease (PSI) Metallohydrolase/ oxidoreductase

DNA catabolism and RNA catabolism are two process terms annotating the co- purifying protein Nuc1. YKR079c also copurifies and and colocalizes to the nucleus with YMR099c which has an unknown process.

YLL034C

Bio. Proc.:	Organelle organization and biogenesis
Cell. Comp.:	Nucleolus, nucleus
Mol. Func.:	ATPase

Mass Spec.:	Organelle organization and biogenesis
Localization:	Nucleolus, nucleus
Two-Hybrid:	None
Struc. Pred.:	Membrane fusion ATPase

None

YLL037W

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No positives
Struc. Pred.:	None

YLR008C (PAM18)

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Chaperone

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	None
Struc. Pred.:	Chaperone J- domain

None

YLR022C

Bio. Proc.:	Unknown
Cell. Comp.:	Cytoplasm
Mol. Func.:	Unknown

Mass Spec.:	None
Localization:	Cytoplasm
Two-Hybrid:	None
Struc. Pred.:	None

None

YLR051C

Bio. Proc.:	Unknown
Cell. Comp.:	Nucleolus, nucleus
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Nucleolus, nucleus
Two-Hybrid:	No positives
Struc. Pred.:	None

None

YLR132C

Bio. Proc.:	mRNA splicing
Cell. Comp.:	Nucleus, mitochondrion
Mol. Func.:	Unknown

Mass Spec.:	mRNA splicing
Localization:	Nucleus, mitochondrion
Two-Hybrid:	No positives
Struc. Pred.:	None

GO process is based on the co- purifying proteins from the nucleus. Mitochondrial protein that co- purified was Cor1 which is involved in aerobic respiration.

YLR145W

Bio. Proc.:	tRNA processing
Cell. Comp.:	Nucleolus, nucleus
Mol. Func.:	Unknown

Mass Spec.:	tRNA processing
Localization:	Nucleolus, nucleus
Two-Hybrid:	No positives
Struc. Pred.:	None

New component of Rnase P tRNA processing complex. YLR145w can be purified but is difficult to detect by mass spectrometry.

YLR243W

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Signal peptide binding

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	Signal peptide- binding domain superfamily; GTPase domain of the signal sequence recognition protein Ffh

None

YLR424W

Bio. Proc.:	mRNA splicing
Cell. Comp.:	Nucleus¹
Mol. Func.:	RNA binding

Mass Spec.:	mRNA splicing
Localization:	No signal
Two-Hybrid:	Nucleobase, nucleoside, nucleotide and nucleic acid metabolism *
Struc. Pred.:	G- patch domain protein family (RNA binding)

Interaction between YLR424w and YKR022c was detected by both two- hybrid and mass spectrometry.

YLR440C

Bio. Proc.:	Unknown
Cell. Comp.:	Nuclear envelope- endoplasmic reticulum network
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Nuclear envelope- endoplasmic reticulum network
Two-Hybrid:	No data
Struc. Pred.:	ARM repeat superfamily

None

YML023C

Bio. Proc.:	DNA repair
Cell. Comp.:	Nucleus
Mol. Func.:	Unknown

Mass Spec.:	DNA repair
Localization:	Nucleus
Two-Hybrid:	Cell growth and/ or maintenance *
Struc. Pred.:	None

None

YMR134W

Bio. Proc.:	Unknown
Cell. Comp.:	Nuclear envelope- endoplasmic reticulum network
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Nuclear envelope- endoplasmic reticulum network
Two-Hybrid:	No data
Struc. Pred.:	None

None

YMR185W

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	Ty element transposition
Localization:	No signal
Two-Hybrid:	No data
Struc. Pred.:	ARM repeat superfamily

Ty elements seem to purify with several unrelated TAP- tagged proteins - YDR288w, YPL063w, YKL195w so they were not used to assign an annotation.

YMR211W (DML1)

Bio. Proc.:	Unknown
Cell. Comp.:	Mitochondrion, cytoplasm
Mol. Func.:	Structural molecule

Mass Spec.:	No data
Localization:	Mitochondrion, cytoplasm
Two-Hybrid:	None
Struc. Pred.:	Tubulin: stathmin- like domain complex

None

YMR298W

Bio. Proc.:	Unknown
Cell. Comp.:	Nuclear envelope- endoplasmic reticulum network
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Nuclear envelope- endoplasmic reticulum network
Two-Hybrid:	No data
Struc. Pred.:	None

None

YNL026W (SAM50)

Bio. Proc.:	Unknown
Cell. Comp.:	Mitochondrion
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Mitochondrion
Two-Hybrid:	No data
Struc. Pred.:	None

None

YNL124W (NAF1)

Bio. Proc.:	rRNA processing
Cell. Comp.:	Nucleolus, nucleus
Mol. Func.:	Unknown

Mass Spec.:	rRNA processing
Localization:	Nucleolus, nucleus
Two-Hybrid:	None
Struc. Pred.:	Beta and beta- prime subunits of DNA dependent RNA- polymerase superfamily

Psi- blast match was for a 146 amino acid stretch of RPB1. This stretch of amino acids has been implicated to be involved in protein- protein interactions with RPB2 and RPB6.

YNL149C

Bio. Proc.:	Unknown
Cell. Comp.:	Nuclear envelope- endoplasmic reticulum network
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Nuclear envelope- endoplasmic reticulum network
Two-Hybrid:	No data
Struc. Pred.:	Winged helix DNA- binding domain superfamily

PSP is not consistent with localization.

YNL150W

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	None

YNL152W

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Phospholipid binding

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	Synaptogamin C2 domain; NEDD4 WWIII domain

None

YNL158W

Bio. Proc.:	Unknown
Cell. Comp.:	Nuclear membrane
Mol. Func.:	Unknown

Mass Spec.:	Unknown
Localization:	Nuclear membrane
Two-Hybrid:	Unknown
Struc. Pred.:	None

None

YNL181W

Bio. Proc.:	Unknown
Cell. Comp.:	Nuclear envelope- endoplasmic reticulum network
Mol. Func.:	Oxidoreductase

Mass Spec.:	None
Localization:	Nuclear envelope- endoplasmic reticulum network
Two-Hybrid:	No data
Struc. Pred.:	Tyrosine- dependent oxidoreductases

None

YNL182C (IPI3)

Bio. Proc.:	Unknown
Cell. Comp.:	Nucleus
Mol. Func.:	Unknown

Mass Spec.:	Unknown complex 2
Localization:	Nucleus
Two-Hybrid:	Unknown complex 2^*
Struc. Pred.:	Tup1, C- terminal domain; WD40 repeat superfamily

None

YNL207W (RIO2)

Bio. Proc.:	Unknown
Cell. Comp.:	Cytoplasm
Mol. Func.:	Protein kinase

Mass Spec.:	None
Localization:	Cytoplasm
Two-Hybrid:	None
Struc. Pred.:	Chk2 kinase; Cyclin- dependent PK

None

YNL245C (CWC25)

Bio. Proc.:	mRNA splicing
Cell. Comp.:	Nucleus
Mol. Func.:	Unknown

Mass Spec.:	mRNA splicing
Localization:	Nucleus
Two-Hybrid:	None
Struc. Pred.:	None

None

YNL260C

Bio. Proc.:	Unknown
Cell. Comp.:	Cytoplasm, nucleus
Mol. Func.:	Unknown

Mass Spec.:	Unknown complex 3
Localization:	Cytoplasm, nucleus
Two-Hybrid:	No positives
Struc. Pred.:	None

Copurification with another essential uncharacerized protein YJR067c.

YNL310C

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No signal
Two-Hybrid:	None
Struc. Pred.:	None

YNL313C

Bio. Proc.:	Nuclear membrane fusion
Cell. Comp.:	Cytoplasm, nucleus
Mol. Func.:	Unknown

Mass Spec.:	Nuclear membrane fusion
Localization:	Cytoplasm, nucleus
Two-Hybrid:	No positives
Struc. Pred.:	ARM repeat superfamily; TPR- like superfamily

None

YNR046W

Bio. Proc.:	Unknown
Cell. Comp.:	Cytoplasm, nucleus, nucleolus
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Cytoplasm, nucleus, nucleolus
Two-Hybrid:	None
Struc. Pred.:	None

None

YNR054C

Bio. Proc.:	rRNA processing
Cell. Comp.:	Nucleolus ¹
Mol. Func.:	RNA binding

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	rRNA processing
Struc. Pred.:	Three RNA binding domains

The GO process was assigned based on the interaction of YNR054c with YDR365c by two- hybrid, which was detected with either YNR054c or YDR365c as the bait.

YOL022C

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	None
Struc. Pred.:	None

YOL026C

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	None

YOL146W (PSF3)

Bio. Proc.:	DNA repair
Cell. Comp.:	Nucleus ¹
Mol. Func.:	Unknown

Mass Spec.:	Unknown complex 1
Localization:	No data
Two-Hybrid:	None
Struc. Pred.:	Ribosome recycling factor

Co- purification with TAP- tagged YJL072c allowed placement into unknown complex 1. The structure prediction of ribosome recycling factor is not consistent with the process and component annotations and was not used to annotate function.

YOR004W

Bio. Proc.:	Unknown
Cell. Comp.:	Nucleolus
Mol. Func.:	Unknown

Mass Spec.:	None
Localization:	Nucleolus
Two-Hybrid:	No data
Struc. Pred.:	None

None

YOR060C

Bio. Proc.:	Unknown
Cell. Comp.:	Cytoplasm, nucleus, nuclear envelope- endoplasmic reticulum network
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Cytoplasm, nucleus, nuclear envelope- endoplasmic reticulum network
Two-Hybrid:	No data
Struc. Pred.:	None

None

YOR262W

Bio. Proc.:	Unknown
Cell. Comp.:	Cytoplasm
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	Cytoplasm
Two-Hybrid:	None
Struc. Pred.:	Domain of the SRP/ SRP receptor G- proteins

Uncharacterized essential ORF YJR072c as a bait detects YOR262w prey in two- hybrid analysis. Both proteins have related structure predictions and same localization.

YOR287C

Bio. Proc.:	Sporulation
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	Sporulation
Struc. Pred.:	Tropomyosin superfamily

None

YPL063W (TIM50)

Bio. Proc.:	Mitochondrial translocation
Cell. Comp.:	Mitochondrion
Mol. Func.:	Unknown

Mass Spec.:	Mitochondrial translocation
Localization:	Mitochondrion
Two-Hybrid:	Transport
Struc. Pred.:	NLI interacting factor protein family, 4- helical cytokines superfamily

Assignment of process based on co- purification with TOM40 and localization.

YPL146C

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	None
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	Winged helix DNA- binding domain superfamily

YPL233W (NSL1)

Bio. Proc.:	Chromosome segregation
Cell. Comp.:	Kinetochore
Mol. Func.:	Unknown

Mass Spec.:	Chromosome segregation
Localization:	Kinetochore
Two-Hybrid:	None
Struc. Pred.:	Spectrin repeat

Nsl1 is an activator as the bait in a two- hybrid screen.

YPR085C

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No signal
Two-Hybrid:	No data
Struc. Pred.:	WD40 repeat superfamily

YPR143W

Bio. Proc.:	Unknown
Cell. Comp.:	Unknown
Mol. Func.:	Unknown

Mass Spec.:	No data
Localization:	No data
Two-Hybrid:	No data
Struc. Pred.:	Tropomyosin superfamily

YPR169W

Bio. Proc.:	Protein mono- ubiquitination
Cell. Comp.:	Nucleolus, nucleus
Mol. Func.:	Unknown

Mass Spec.:	Protein mono- ubiquitination
Localization:	Nucleolus, nucleus
Two-Hybrid:	No positives
Struc. Pred.:	WD40 repeat superfamily

Possible molecular function annotation based on copurification with Bre1 would be ubiquitin- protein ligase activity.

Assignment of GO terms based on the experimental and computational evidence from the four technologies. The GO term finder (LINK) was used to predict biological processes from the co-purification or two-hybrid data sets. Localization was a direct assay that determined the component term. Protein structure prediction and remote homology searches were used to annotate for the molecular function term. (PSI indicates a remote homology search; ER, endoplasmic reticulum network; *, overlap between the co-purification and two-hybrid data sets)

¹Component term was assigned from co-purification or two-hybrid data

²Process term was assigned from remote homology searches or protein structure prediction

³Function term was assigned from two-hybrid data



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