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COPURIFYING SETS OF PROTEINS1

Search complexes for ORF/Gene (e.g. 'DSN1'):


* denotes mass spectrometry bait

1. YFR003C Cell cycle:


2. YIR010W (DSN1) and YPL233W (NSL1) Chromosome segregation:


3. YKL088W Coenzyme A biosynthesis2:


4. YKR079C DNA or RNA catabolism3:


5. YJL072C DNA repair (unknown complex 1):


6. YDR288W DNA repair:


7. YML023C DNA repair:


8. YGR002C Histone acetylation:


9. YGR198W MAPKKK cascade:


10. YPL063W (TIM50) Mitochondrial translocation2:


11. YGR046W Mitochondrial translocation2:


12. YDL209C (CWC2) mRNA splicing:


13. YLR424W and YKR022C mRNA splicing:


14. YLR132C mRNA splicing3:


15. YNL245C (CWC25) mRNA splicing2:


16. YNL313C Nuclear membrane fusion:


17. YLL034C Organelle organization and biogenesis4:


18. YJR136C Protein Biosynthesis:


19. YPR169W Protein monoubiquitination:


20. YKL195W Protein targeting:


21. YKR038C Response to dessication:


22. YNL124W rRNA processing:


23. YJL010C rRNA processing:


24. YDR365C and YGR145W rRNA processing:


25. YJL091C Secretory pathway:


26. YJR012C Transport:


27. YNL221C (POP1) tRNA processing5:


28. YHR085W and YHR197W (RIX1) and YNL182C Unknown Complex 2:


29. YNL260C Unknown Complex 3:


1The set of proteins co-purifying with a given uncharacterized protein was defined using the algorithm described in Experimental Procedures except as noted.

2For these ORFs, the co-purifying set of proteins were the proteins with high coverage in the mass spectrometric analysis.

3The localization and mass spectrometry data suggest that the asterisked protein forms two complexes, one in the mitochondrion and one in the nucleus (see manuscript text).

4The GO annotation was chosen as the significant annotation involving more than 2 of the ORFs.

5The POP1 complex was purified using TAP tagged-YLR145W, an uncharacterized essential protein. Although used as the bait, YLR145W was detected in only 1 of 3 mass spectrometric analyses.