YRC Public Data Repository - Protein Structure Prediction

Protein:	PRIM_ECOLI
Organism:	Escherichia coli
Length:	581 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PRIM_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|75176041, gi\|... - gi\|75176041\|ref\|ZP_00696209.1\| COG0358: DNA primase (bacterial type) [Shigella boydii BS512], gi\|187...	563.0	[0..1]	[581..1]
gi\|91212495, gi\|... - gi\|91212495\|ref\|YP_542481.1\| DNA primase [Escherichia coli UTI89], gi\|91074069\|gb\|ABE08950.1\| DNA pr... gi\|115514502, gi... - gi\|117625378\|ref\|YP_855495.1\| DNA primase [Escherichia coli APEC O1], gi\|115514502\|gb\|ABJ02577.1\| DN... gi\|218560153, gi... - gi\|218560153\|ref\|YP_002393066.1\| DNA primase [Escherichia coli S88], gi\|218366922\|emb\|CAR04693.1\| DN... DNAG_ECOL6 - DNA primase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) GN=dnaG PE=3 SV=1 gi\|237706171, gi... - gi\|237706171\|ref\|ZP_04536652.1\| DNA primase [Escherichia sp. 3_2_53FAA], gi\|226899211\|gb\|EEH85470.1\|... gi\|227887790, gi... - gi\|227887790\|ref\|ZP_04005595.1\| DNA primase [Escherichia coli 83972], gi\|227835186\|gb\|EEJ45652.1\| DN... PRIM_ECOL6 - DNA primase OS=Escherichia coli O6 GN=dnaG PE=3 SV=1	562.0	[0..1]	[581..1]
gi\|75230016 - gi\|75230016\|ref\|ZP_00716529.1\| COG0358: DNA primase (bacterial type) [Escherichia coli B7A]	562.0	[0..1]	[581..2]
gi\|81246732, gi\|... - gi\|82545321\|ref\|YP_409268.1\| DNA primase [Shigella boydii Sb227], gi\|81246732\|gb\|ABB67440.1\| DNA pri...	562.0	[0..1]	[581..1]
gi\|75259999, gi\|... - gi\|75259999\|ref\|ZP_00731277.1\| COG0358: DNA primase (bacterial type) [Escherichia coli E22], gi\|1930...	562.0	[0..1]	[581..1]
gi\|82778396, gi\|... - gi\|82778396\|ref\|YP_404745.1\| DNA primase [Shigella dysenteriae Sd197], gi\|81242544\|gb\|ABB63254.1\| DN...	562.0	[0..1]	[581..1]
gi\|75240902 - gi\|75240902\|ref\|ZP_00724800.1\| COG0358: DNA primase (bacterial type) [Escherichia coli F11]	561.0	[0..1]	[581..1]
gi\|110616501, gi... - gi\|110806953\|ref\|YP_690473.1\| DNA primase [Shigella flexneri 5 str. 8401], gi\|110616501\|gb\|ABF05168....	560.0	[0..1]	[581..1]
gi\|15803608, gi\|... - gi\|15803608\|ref\|NP_289641.1\| DNA primase [Escherichia coli O157:H7 EDL933], gi\|12517648\|gb\|AAG58200.... gi\|25298043 - gi\|25298043\|pir\|\|D85967 DNA biosynthesis, DNA primase [imported] - Escherichia coli (strain O157:H7...	560.0	[0..1]	[581..1]
gi\|169753610, gi... - gi\|170018682\|ref\|YP_001723636.1\| DNA primase [Escherichia coli ATCC 8739], gi\|169753610\|gb\|ACA76309.... gi\|188494232, gi... - gi\|188494232\|ref\|ZP_03001502.1\| DNA primase [Escherichia coli 53638], gi\|188489431\|gb\|EDU64534.1\| DN... gi\|75197020, gi\|... - gi\|75197020\|ref\|ZP_00707090.1\| COG0358: DNA primase (bacterial type) [Escherichia coli HS], gi\|15716... gi\|124502127, gi... - gi\|124526023\|ref\|ZP_01698028.1\| DNA primase [Escherichia coli B], gi\|124502127\|gb\|EAY49587.1\| DNA pr...	559.0	[0..1]	[581..1]

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Predicted Domain #1

Region A:
Residues: [1-105]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAGRIPRVFI NDLLARTDIV DLIDARVKLK KQGKNFHACC PFHNEKTPSF TVNGEKQFYH  60
   61 CFGCGAHGNA IDFLMNYDKL EFVETVEELA AMHNLEVPFE AGSGP

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	35.154902
Match:	1d0qA
Description:	Zinc-binding domain of DNA primase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
DNA primase activity	9.73419395042405	bayes_pls_golite062009
transcription regulator activity	3.46382129825313	bayes_pls_golite062009
DNA-directed RNA polymerase activity	3.39768224850127	bayes_pls_golite062009
RNA polymerase activity	3.39768224850127	bayes_pls_golite062009
nucleotidyltransferase activity	3.28799371541043	bayes_pls_golite062009
binding	2.62275087285714	bayes_pls_golite062009
nucleic acid binding	1.95935440221316	bayes_pls_golite062009
DNA binding	1.92092218509957	bayes_pls_golite062009
transcription elongation regulator activity	1.49626290953998	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	1.23314259311645	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.730134088263314	bayes_pls_golite062009
catalytic activity	0.434839928397784	bayes_pls_golite062009
protein binding	0.265222284938592	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.240481799431949	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [106-444]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SQIERHQRQT LYQLMDGLNT FYQQSLQQPV ATSARQYLEK RGLSHEVIAR FAIGFAPPGW  60
   61 DNVLKRFGGN PENRQSLIDA GMLVTNDQGR SYDRFRERVM FPIRDKRGRV IGFGGRVLGN 120
  121 DTPKYLNSPE TDIFHKGRQL YGLYEAQQDN AEPNRLLVVE GYMDVVALAQ YGINYAVASL 180
  181 GTSTTADHIQ LLFRATNNVI CCYDGDRAGR DAAWRALETA LPYMTDGRQL RFMFLPDGED 240
  241 PDTLVRKEGK EAFEARMEQA MPLSAFLFNS LMPQVDLSTP DGRARLSTLA LPLISQVPGE 300
  301 TLRIYLRQEL GNKLGILDDS QLERLMPKAA ESGVSRPVP

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	83.0
Match:	1eqnA
Description:	DNA primase DnaG catalytic core
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
DNA primase activity	11.9648478190162	bayes_pls_golite062009
DNA-directed RNA polymerase activity	4.35308216592295	bayes_pls_golite062009
RNA polymerase activity	4.35308216592295	bayes_pls_golite062009
nucleotidyltransferase activity	4.2392655850006	bayes_pls_golite062009
binding	2.46518385502315	bayes_pls_golite062009
catalytic activity	1.39967796334655	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.17252562283519	bayes_pls_golite062009
transferase activity	0.605239890876961	bayes_pls_golite062009
protein binding	0.11913220264667	bayes_pls_golite062009

Predicted Domain #3

Region A:
Residues: [445-581]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QLKRTTMRIL IGLLVQNPEL ATLVPPLENL DENKLPGLGL FRELVNTCLS QPGLTTGQLL  60
   61 EHYRGTNNAA TLEKLSMWDD IADKNIAEQT FTDSLNHMFD SLLELRQEEL IARERTHGLS 120
  121 NEERLELWTL NQELAKK

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	27.0
Match:	1t3wA
Description:	Crystal Structure of the E.coli DnaG C-terminal domain (residues 434 to 581)
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions:

Predicted functions: