YRC Public Data Repository - Protein Structure Prediction

Protein:	NEMA_ECOLI
Organism:	Escherichia coli
Length:	365 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for NEMA_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|77959187 - gi\|77959187\|ref\|ZP_00823209.1\| COG1902: NADH:flavin oxidoreductases, Old Yellow Enzyme family [Yersi...	419.0	[0..1]	[365..1]
gi\|77961716 - gi\|77961716\|ref\|ZP_00825548.1\| COG1902: NADH:flavin oxidoreductases, Old Yellow Enzyme family [Yersi...	417.0	[0..1]	[365..1]
gi\|238787145, gi... - gi\|77975305\|ref\|ZP_00830841.1\| COG1902: NADH:flavin oxidoreductases, Old Yellow Enzyme family [Yersi...	416.0	[0..1]	[365..1]
gi\|238792289, gi... - gi\|77979560\|ref\|ZP_00834978.1\| COG1902: NADH:flavin oxidoreductases, Old Yellow Enzyme family [Yersi...	416.0	[0..1]	[365..1]
gi\|77633724, gi\|... - gi\|77633724\|ref\|ZP_00795843.1\| COG1902: NADH:flavin oxidoreductases, Old Yellow Enzyme family [Yersi... gi\|153950748, gi... - gi\|77629364\|ref\|ZP_00791950.1\| COG1902: NADH:flavin oxidoreductases, Old Yellow Enzyme family [Yersi... gi\|165990445, gi... - gi\|166010743\|ref\|ZP_02231641.1\| oxidoreductase, FAD/FMN-binding [Yersinia pestis biovar Antiqua str.... gi\|165921640, gi... - gi\|165926419\|ref\|ZP_02222251.1\| oxidoreductase, FAD/FMN-binding [Yersinia pestis biovar Orientalis s... gi\|149292123, gi... - gi\|149365710\|ref\|ZP_01887745.1\| NADH:flavin oxidoreductase / NADH oxidase family protein [Yersinia p... gi\|145598070, gi... - gi\|145598070\|ref\|YP_001162146.1\| N-ethylmaleimide reductase [Yersinia pestis Pestoides F], gi\|145209... gi\|108807720, gi... - gi\|108807720\|ref\|YP_651636.1\| N-ethylmaleimide reductase [Yersinia pestis Antiqua], gi\|108779633\|gb\|... gi\|229680641, gi... - gi\|229902306\|ref\|ZP_04517426.1\| NADH:flavin oxidoreductase / NADH oxidase family protein [Yersinia p... gi\|89103792, gi\|... - gi\|89103792\|ref\|ZP_01176356.1\| COG1902: NADH:flavin oxidoreductases, Old Yellow Enzyme family [Yersi... gi\|167400926, gi... - gi\|167400926\|ref\|ZP_02306432.1\| oxidoreductase, FAD/FMN-binding [Yersinia pestis biovar Antiqua str.... gi\|166207777, gi... - gi\|166210597\|ref\|ZP_02236632.1\| oxidoreductase, FAD/FMN-binding [Yersinia pestis biovar Antiqua str.... gi\|25325966 - pir\|\|AD0290 NADH flavin oxidoreductase / NADH oxidase family protein YPO2379 [imported] - Yersinia p... gi\|167470352 - gi\|167470352\|ref\|ZP_02335056.1\| oxidoreductase, FAD/FMN-binding protein [Yersinia pestis FV-1] gi\|229837903, gi... - gi\|229837903\|ref\|ZP_04458062.1\| NADH:flavin oxidoreductase / NADH oxidase family protein [Yersinia p... gi\|166958908, gi... - gi\|167422096\|ref\|ZP_02313849.1\| oxidoreductase, FAD/FMN-binding [Yersinia pestis biovar Orientalis s... gi\|229701952, gi... - gi\|229895064\|ref\|ZP_04510241.1\| NADH:flavin oxidoreductase / NADH oxidase family protein [Yersinia p... gi\|218929470, gi... - gi\|218929470\|ref\|YP_002347345.1\| N-ethylmaleimide reductase [Yersinia pestis CO92], gi\|16122602\|ref\|... gi\|229688510, gi... - gi\|229898466\|ref\|ZP_04513612.1\| NADH:flavin oxidoreductase / NADH oxidase family protein [Yersinia p... gi\|45441957, gi\|... - gi\|45441957\|ref\|NP_993496.1\| N-ethylmaleimide reductase [Yersinia pestis biovar Microtus str. 91001]... gi\|170024110, gi... - gi\|170024110\|ref\|YP_001720615.1\| N-ethylmaleimide reductase [Yersinia pseudotuberculosis YPIII], gi\|...	414.0	[0..1]	[365..1]
gi\|56127852, gi\|... - gi\|56413595\|ref\|YP_150670.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica serovar... gi\|197093996, gi... - gi\|197362519\|ref\|YP_002142156.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica ser... gi\|168264729, gi... - gi\|205346855\|gb\|EDZ33486.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica serovar ...	413.0	[0..1]	[365..1]
gi\|16502773, gi\|... - gi\|25325970\|pir\|\|AB0695 N-ethylmaleimide reductase (EC 1.-.-.-) [imported] - Salmonella enterica sub... gi\|213586463 - gi\|213586463\|ref\|ZP_03368289.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica sero... gi\|29137391, gi\|... - gi\|29141763\|ref\|NP_805105.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica serovar... gi\|213426001 - gi\|213426001\|ref\|ZP_03358751.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica sero... gi\|213161994 - gi\|213161994\|ref\|ZP_03347704.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica sero... gi\|16760477 - gi\|16760477\|ref\|NP_456094.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica serovar... gi\|214000293 - gi\|214000293\|ref\|ZP_03411028.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica sero... gi\|213855185 - gi\|213855185\|ref\|ZP_03383425.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica sero...	413.0	[0..1]	[365..1]
gi\|186895672, gi... - gi\|186895672\|ref\|YP_001872784.1\| N-ethylmaleimide reductase [Yersinia pseudotuberculosis PB1/+], gi\|... gi\|51589899, gi\|... - gi\|51596617\|ref\|YP_070808.1\| N-ethylmaleimide reductase [Yersinia pseudotuberculosis IP 32953], gi\|5... Q66A39\|Q66A39_YERPS - NADH:flavin oxidoreductase / NADH oxidase family protein - Yersinia pseudotuberculosis	412.0	[0..1]	[365..1]
gi\|194405865, gi... - gi\|194447561\|ref\|YP_002045474.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica ser... gi\|197242562, gi... - gi\|197264307\|ref\|ZP_03164381.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica sero... gi\|205327852, gi... - gi\|205327852\|gb\|EDZ14616.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica serovar ... gi\|168240950, gi... - gi\|205339189\|gb\|EDZ25953.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica serovar ... gi\|16419956, gi\|... - gi\|16764784\|ref\|NP_460399.1\| N-ethylmaleimide reductase [Salmonella typhimurium LT2], gi\|16419956\|gb... lcl\|NC_016856.1_... - [gene=nemA] [protein=N-ethylmaleimide reductase] [protein_id=YP_005237306.1] [location=complement(15...	410.0	[0..1]	[365..1]
gi\|62180025, gi\|... - gi\|62180025\|ref\|YP_216442.1\| N-ethylmaleimide reductase [Salmonella enterica subsp. enterica serovar...	409.0	[0..1]	[365..1]

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Predicted Domain #1

Region A:
Residues: [1-365]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSEKLYSPL KVGAITAANR IFMAPLTRLR SIEPGDIPTP LMAEYYRQRA SAGLIISEAT  60
   61 QISAQAKGYA GAPGIHSPEQ IAAWKKITAG VHAENGHMAV QLWHTGRISH ASLQPGGQAP 120
  121 VAPSALSAGT RTSLRDENGQ AIRVETSMPR ALELEEIPGI VNDFRQAIAN AREAGFDLVE 180
  181 LHSAHGYLLH QFLSPSSNHR TDQYGGSVEN RARLVLEVVD AGIEEWGADR IGIRVSPIGT 240
  241 FQNTDNGPNE EADALYLIEQ LGKRGIAYLH MSEPDWAGGE PYTDAFREKV RARFHGPIIG 300
  301 AGAYTVEKAE TLIGKGLIDA VAFGRDWIAN PDLVARLQRK AELNPQRAES FYGGGAEGYT 360
  361 DYPTL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	113.0
Match:	1gvoA
Description:	Pentaerythritol tetranirate reductase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
oxidoreductase activity, acting on the CH-CH group of donors	2.11027907327307	bayes_pls_golite062009
catalytic activity	1.94749091175302	bayes_pls_golite062009
binding	1.2785695107981	bayes_pls_golite062009
coenzyme binding	0.99234662301893	bayes_pls_golite062009
oxidoreductase activity	0.84996661470385	bayes_pls_golite062009
nucleic acid binding	0.350068675013616	bayes_pls_golite062009
oxidoreductase activity, acting on CH-OH group of donors	0.0276497014195508	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: