YRC Public Data Repository - Protein Structure Prediction

Protein:	NADE_ECOLI
Organism:	Escherichia coli
Length:	275 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

[What does the above image mean?]

[Show Ginzu Version Information]

Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for NADE_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|253773306, gi... - gi\|254288587\|ref\|YP_003054335.1\| NAD synthetase [Escherichia coli BL21(DE3)], gi\|253977894\|gb\|ACT435... gi\|242377461, gi... - gi\|251785188\|ref\|YP_002999492.1\| hypothetical protein B21_01697 [Escherichia coli BL21], gi\|24237746... gi\|254161799, gi... - gi\|254161799\|ref\|YP_003044907.1\| NAD synthetase [Escherichia coli B str. REL606], gi\|253973700\|gb\|AC... gi\|194438578, gi... - gi\|83586039\|ref\|ZP_00924677.1\| COG0171: NAD synthase [Escherichia coli 101-1], gi\|194438578\|ref\|ZP_0...	289.0	[0..1]	[275..1]
NADE_ECOK1 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O1:K1 / APEC GN=nadE PE=3 SV=1 NADE_ECOUT - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli (strain UTI89 / UPEC) GN=nadE PE=3 SV=1 gi\|226900447, gi... - gi\|237705690\|ref\|ZP_04536171.1\| NH(3)-dependent NAD(+) synthetase [Escherichia sp. 3_2_53FAA], gi\|22... NADE_ECO45 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=nadE PE=3 SV=1	288.0	[0..1]	[275..1]
NADE_ECO7I - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=nadE PE=3 SV=1 NADE_ECO55 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli (strain 55989 / EAEC) GN=nadE PE=3 SV=1 NADE_ECO81 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O81 (strain ED1a) GN=nadE PE=3 SV=1 gi\|194418241, gi... - gi\|83569656\|ref\|ZP_00921107.1\| COG0171: NAD synthase [Shigella dysenteriae 1012], gi\|194433478\|ref\|Z...	288.0	[0..1]	[275..1]
gi\|75227067, gi\|... - gi\|75227067\|ref\|ZP_00713891.1\| COG0171: NAD synthase [Escherichia coli B7A], gi\|191169567\|ref\|ZP_030...	288.0	[0..1]	[275..1]
gi\|75256142, gi\|... - gi\|75256142\|ref\|ZP_00727838.1\| COG0171: NAD synthase [Escherichia coli E22], gi\|193065791\|ref\|ZP_030... gi\|75210227, gi\|... - gi\|75210227\|ref\|ZP_00710395.1\| COG0171: NAD synthase [Escherichia coli B171], gi\|194428548\|ref\|ZP_03...	288.0	[0..1]	[275..1]
gi\|75241385, gi\|... - gi\|75241385\|ref\|ZP_00725236.1\| COG0171: NAD synthase [Escherichia coli F11], gi\|191171747\|ref\|ZP_030... NADE_ECOL5 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=nadE PE=3 SV... gi\|227885836, gi... - gi\|227885836\|ref\|ZP_04003641.1\| NAD(+) synthase [Escherichia coli 83972], gi\|227837409\|gb\|EEJ47875.1... NADE_ECOL6 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) GN=... NADE_ECOL6 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O6 GN=nadE PE=3 SV=1	287.0	[0..1]	[275..1]
NADE_ECO24 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=nadE PE=3 ... NADE_ECOSE - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli (strain SE11) GN=nadE PE=3 SV=1 NADE_SHISS - NH(3)-dependent NAD(+) synthetase OS=Shigella sonnei (strain Ss046) GN=nadE PE=3 SV=1	287.0	[0..1]	[275..1]
NADE_PECAS - NH(3)-dependent NAD(+) synthetase OS=Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) G... NADE_ERWCT - NH(3)-dependent NAD(+) synthetase OS=Erwinia carotovora subsp. atroseptica GN=nadE PE=3 SV=1	287.0	[0..1]	[274..1]
NADE_SHIDS - NH(3)-dependent NAD(+) synthetase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=nadE PE=3 SV=...	287.0	[0..1]	[275..1]
gi\|192957766, gi... - gi\|75236791\|ref\|ZP_00720862.1\| COG0171: NAD synthase [Escherichia coli E110019], gi\|193068971\|ref\|ZP...	287.0	[0..1]	[275..1]

Back

Predicted Domain #1

Region A:
Residues: [1-275]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTLQQQIIKA LGAKPQINAE EEIRRSVDFL KSYLQTYPFI KSLVLGISGG QDSTLAGKLC  60
   61 QMAINELRLE TGNESLQFIA VRLPYGVQAD EQDCQDAIAF IQPDRVLTVN IKGAVLASEQ 120
  121 ALREAGIELS DFVRGNEKAR ERMKAQYSIA GMTSGVVVGT DHAAEAITGF FTKYGDGGTD 180
  181 INPLYRLNKR QGKQLLAALA CPEHLYKKAP TADLEDDRPS LPDEVALGVT YDNIDDYLEG 240
  241 KNVPQQVART IENWYLKTEH KRRPPITVFD DFWKK

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	77.0
Match:	1wxeA
Description:	E.coli NAD Synthetase, AMP
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
ligase activity, forming carbon-nitrogen bonds	1.9422825865652	bayes_pls_golite062009
carbon-nitrogen ligase activity, with glutamine as amido-N-donor	1.77152913449838	bayes_pls_golite062009
NAD+ synthase (glutamine-hydrolyzing) activity	1.13284666503836	bayes_pls_golite062009
GMP synthase (glutamine-hydrolyzing) activity	1.08094655947236	bayes_pls_golite062009
ligase activity	1.02544349488516	bayes_pls_golite062009
catalytic activity	0.828867994386867	bayes_pls_golite062009
NAD+ synthase activity	0.693146657234538	bayes_pls_golite062009
binding	0.268276895381766	bayes_pls_golite062009
protein binding	0.0198162482538781	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: