YRC Public Data Repository - Protein Structure Prediction

Protein:	MURF_ECOLI
Organism:	Escherichia coli
Length:	452 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MURF_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|2177096 - gi\|2177096\|gb\|AAB60788.1\| UDP-MurNAc-Tripeptide:D-Ala-D-Ala-Adding Enzyme [Escherichia coli]	383.0	[0..1]	[452..1]
gi\|50122741, gi\|... - gi\|50122741\|ref\|YP_051908.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Pectobacter...	378.0	[0..1]	[452..1]
gi\|190905112, gi... - gi\|75235751\|ref\|ZP_00719912.1\| COG0770: UDP-N-acetylmuramyl pentapeptide synthase [Escherichia coli ... gi\|110341891, gi... - gi\|110640299\|ref\|YP_668027.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Escherichi...	375.0	[0..1]	[452..1]
gi\|117622372, gi... - gi\|117622372\|ref\|YP_851285.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Escherichi... gi\|91070724, gi\|... - gi\|91209150\|ref\|YP_539136.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Escherichia... gi\|218363795, gi... - gi\|218557026\|ref\|YP_002389939.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Escheri... gi\|237704235, gi... - gi\|237704235\|ref\|ZP_04534716.1\| UDP-N-acetylmuramoylalanyl-D-glutamyl-2 [Escherichia sp. 3_2_53FAA],...	374.0	[0..1]	[452..1]
gi\|75234323, gi\|... - gi\|75234323\|ref\|ZP_00718701.1\| COG0770: UDP-N-acetylmuramyl pentapeptide synthase [Escherichia coli ... gi\|192926478, gi... - gi\|75256042\|ref\|ZP_00727755.1\| COG0770: UDP-N-acetylmuramyl pentapeptide synthase [Escherichia coli ... gi\|194429369, gi... - gi\|75209393\|ref\|ZP_00709625.1\| COG0770: UDP-N-acetylmuramyl pentapeptide synthase [Escherichia coli ... gi\|190902185, gi... - gi\|75229065\|ref\|ZP_00715645.1\| COG0770: UDP-N-acetylmuramyl pentapeptide synthase [Escherichia coli ... gi\|81244101, gi\|... - gi\|82542690\|ref\|YP_406637.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Shigella bo... gi\|188493824, gi... - gi\|75511690\|ref\|ZP_00734328.1\| COG0770: UDP-N-acetylmuramyl pentapeptide synthase [Escherichia coli ... gi\|73854182, gi\|... - gi\|74310705\|ref\|YP_309124.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Shigella so... gi\|169756486, gi... - gi\|170021558\|ref\|YP_001726512.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Escheri... gi\|218350287, gi... - gi\|218693555\|ref\|YP_002401222.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Escheri... gi\|209910538, gi... - gi\|209917279\|ref\|YP_002291363.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Escheri... gi\|124530653, gi... - gi\|124530653\|ref\|ZP_01701237.1\| UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-... gi\|218552669, gi... - gi\|218552669\|ref\|YP_002385582.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Escheri... gi\|157080826, gi... - gi\|75189186\|ref\|ZP_00702453.1\| COG0770: UDP-N-acetylmuramyl pentapeptide synthase [Escherichia coli ... gi\|253976318, gi... - gi\|254287011\|ref\|YP_003052759.1\| UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D- alanine ligase [Escheri... gi\|157159557, gi... - gi\|75194303\|ref\|ZP_00704373.1\| COG0770: UDP-N-acetylmuramyl pentapeptide synthase [Escherichia coli ... gi\|251783649, gi... - gi\|251783649\|ref\|YP_002997953.1\| hypothetical protein B21_00086 [Escherichia coli BL21], gi\|24237592... gi\|194421665, gi... - gi\|83585192\|ref\|ZP_00923842.1\| COG0770: UDP-N-acetylmuramyl pentapeptide synthase [Escherichia coli ... gi\|187733976, gi... - gi\|75175643\|ref\|ZP_00695851.1\| COG0770: UDP-N-acetylmuramyl pentapeptide synthase [Shigella boydii B... gi\|254160208, gi... - gi\|254160208\|ref\|YP_003043316.1\| UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase [Escheric...	374.0	[0..1]	[452..1]
gi\|82775493, gi\|... - gi\|82775493\|ref\|YP_401840.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Shigella dy...	373.0	[0..1]	[452..1]
gi\|227885009, gi... - gi\|227885009\|ref\|ZP_04002814.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Escheric... gi\|26246019, gi\|... - gi\|26246019\|ref\|NP_752058.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Escherichia...	373.0	[0..1]	[452..1]
gi\|77958239 - gi\|77958239\|ref\|ZP_00822276.1\| COG0770: UDP-N-acetylmuramyl pentapeptide synthase [Yersinia bercovie...	373.0	[0..1]	[452..1]
gi\|110613698, gi... - gi\|110804150\|ref\|YP_687670.1\| UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Shigella f...	372.0	[0..1]	[452..1]

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Predicted Domain #1

Region A:
Residues: [1-452]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MISVTLSQLT DILNGELQGA DITLDAVTTD TRKLTPGCLF VALKGERFDA HDFADQAKAG  60
   61 GAGALLVSRP LDIDLPQLIV KDTRLAFGEL AAWVRQQVPA RVVALTGSSG KTSVKEMTAA 120
  121 ILSQCGNTLY TAGNLNNDIG VPMTLLRLTP EYDYAVIELG ANHQGEIAWT VSLTRPEAAL 180
  181 VNNLAAAHLE GFGSLAGVAK AKGEIFSGLP ENGIAIMNAD NNDWLNWQSV IGSRKVWRFS 240
  241 PNAANSDFTA TNIHVTSHGT EFTLQTPTGS VDVLLPLPGR HNIANALAAA ALSMSVGATL 300
  301 DAIKAGLANL KAVPGRLFPI QLAENQLLLD DSYNANVGSM TAAVQVLAEM PGYRVLVVGD 360
  361 MAELGAESEA CHVQVGEAAK AAGIDRVLSV GKQSHAISTA SGVGEHFADK TALITRLKLL 420
  421 IAEQQVITIL VKGSRSAAME EVVRALQENG TC

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	103.0
Match:	1gg4A
Description:	UDP-murNac-tripeptide D-alanyl-D-alanine-adding enzyme MurF
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
catalytic activity	1.79187485439607	bayes_pls_golite062009
ligase activity	1.04270019960814	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	1.01758304875788	bayes_pls_golite062009
acid-amino acid ligase activity	1.00563528012713	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: