YRC Public Data Repository - Protein Structure Prediction

Protein:	GLGB_ECOLI
Organism:	Escherichia coli
Length:	728 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for GLGB_ECOLI.

Description	E-value	Query Range	Subject Range
GLGB_ACTSZ - 1,4-alpha-glucan branching enzyme GlgB OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=...	674.0	[0..1]	[727..1]
GLGB_HISS2 - 1,4-alpha-glucan branching enzyme GlgB OS=Histophilus somni (strain 2336) GN=glgB PE=3 SV=1	652.0	[0..1]	[725..1]
GLGB_HAES1 - 1,4-alpha-glucan branching enzyme GlgB OS=Haemophilus somnus (strain 129Pt) GN=glgB PE=3 SV=1	650.0	[0..1]	[725..1]
GLGB_SHIF8 - 1,4-alpha-glucan branching enzyme GlgB OS=Shigella flexneri serotype 5b (strain 8401) GN=glgB PE=3 S...	646.0	[0..1]	[728..1]
GLGB_SHIFL - 1,4-alpha-glucan branching enzyme GlgB OS=Shigella flexneri GN=glgB PE=3 SV=1 gi\|24053909, gi\|... - gi\|24114698\|ref\|NP_709208.1\| glycogen branching enzyme [Shigella flexneri 2a str. 301], gi\|24053909\|... gi\|30043546, gi\|... - gi\|30065284\|ref\|NP_839455.1\| glycogen branching enzyme [Shigella flexneri 2a str. 2457T], gi\|3004354...	646.0	[0..1]	[728..1]
GLGB_ECOK1 - 1,4-alpha-glucan branching enzyme GlgB OS=Escherichia coli O1:K1 / APEC GN=glgB PE=3 SV=1 gi\|218367266, gi... - gi\|218560497\|ref\|YP_002393410.1\| glycogen branching enzyme [Escherichia coli S88], gi\|218367266\|emb\|... GLGB_ECOUT - 1,4-alpha-glucan branching enzyme GlgB OS=Escherichia coli (strain UTI89 / UPEC) GN=glgB PE=3 SV=1 gi\|226902436, gi... - gi\|237703172\|ref\|ZP_04533653.1\| glycogen branching enzyme [Escherichia sp. 3_2_53FAA], gi\|226902436\|...	645.0	[0..1]	[728..1]
GLGB_SHIDS - 1,4-alpha-glucan branching enzyme GlgB OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=glgB PE=...	645.0	[0..1]	[728..1]
GLGB_ECOL6 - 1,4-alpha-glucan branching enzyme GlgB OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC... gi\|227839454, gi... - gi\|227883575\|ref\|ZP_04001380.1\| glycogen branching enzyme [Escherichia coli 83972], gi\|227839454\|gb\|... GLGB_ECOL6 - 1,4-alpha-glucan branching enzyme GlgB OS=Escherichia coli O6 GN=glgB PE=3 SV=1	644.0	[0..1]	[728..1]
gi\|187430631, gi... - gi\|75179878\|ref\|ZP_00699865.1\| COG0296: 1,4-alpha-glucan branching enzyme [Shigella boydii BS512], g... GLGB_SHIBS - 1,4-alpha-glucan branching enzyme GlgB OS=Shigella boydii serotype 4 (strain Sb227) GN=glgB PE=3 SV=...	644.0	[0..1]	[728..1]
gi\|168759463, gi... - gi\|189369766\|gb\|EDU88182.1\| 1,4-alpha-glucan branching enzyme [Escherichia coli O157:H7 str. EC4501]... gi\|168753191, gi... - gi\|189359176\|gb\|EDU77595.1\| 1,4-alpha-glucan branching enzyme [Escherichia coli O157:H7 str. EC4401]... gi\|188018242, gi... - gi\|188018242\|gb\|EDU56364.1\| 1,4-alpha-glucan branching enzyme [Escherichia coli O157:H7 str. EC4113]... gi\|15803941, gi\|... - gi\|15803941\|ref\|NP_289977.1\| glycogen branching enzyme [Escherichia coli O157:H7 EDL933], gi\|1251807... gi\|25286739, gi\|... - pir\|\|F86009 1,4-alpha-glucan branching enzyme [imported] - Escherichia coli (strain O157:H7, substra... gi\|13363751, gi\|... - gi\|15833531\|ref\|NP_312304.1\| glycogen branching enzyme [Escherichia coli O157:H7 str. Sakai], gi\|133... gi\|217324196, gi... - gi\|217324196\|ref\|ZP_03440280.1\| 1,4-alpha-glucan branching enzyme [Escherichia coli O157:H7 str. TW1... gi\|209158502, gi... - gi\|209397102\|ref\|YP_002272873.1\| 1,4-alpha-glucan branching enzyme [Escherichia coli O157:H7 str. EC... gi\|189380085, gi... - gi\|189380085\|gb\|EDU98501.1\| 1,4-alpha-glucan branching enzyme [Escherichia coli O157:H7 str. EC508],... GLGB_ECO57 - 1,4-alpha-glucan branching enzyme GlgB OS=Escherichia coli O157:H7 GN=glgB PE=3 SV=1 gi\|189002581, gi... - gi\|189002581\|gb\|EDU71567.1\| 1,4-alpha-glucan branching enzyme [Escherichia coli O157:H7 str. EC4076]... gi\|187771818, gi... - gi\|187771818\|gb\|EDU35662.1\| 1,4-alpha-glucan branching enzyme [Escherichia coli O157:H7 str. EC4196]... gi\|254795351, gi... - gi\|254795351\|ref\|YP_003080188.1\| 1,4-alpha-glucan branching enzyme [Escherichia coli O157:H7 str. TW... gi\|168765786, gi... - gi\|189364775\|gb\|EDU83194.1\| 1,4-alpha-glucan branching enzyme [Escherichia coli O157:H7 str. EC4486]... gi\|208818838, gi... - gi\|208818838\|ref\|ZP_03259158.1\| 1,4-alpha-glucan branching enzyme [Escherichia coli O157:H7 str. EC4... gi\|208733731, gi... - gi\|208812454\|ref\|ZP_03253783.1\| 1,4-alpha-glucan branching enzyme [Escherichia coli O157:H7 str. EC4... gi\|208808734, gi... - gi\|208808734\|ref\|ZP_03251071.1\| 1,4-alpha-glucan branching enzyme [Escherichia coli O157:H7 str. EC4... gi\|195934924 - gi\|195934924\|ref\|ZP_03080306.1\| glycogen branching enzyme [Escherichia coli O157:H7 str. EC4024]	644.0	[0..1]	[728..1]

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Predicted Domain #1

Region A:
Residues: [1-116]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDRIDRDVI NALIAGHFAD PFSVLGMHKT TAGLEVRALL PDATDVWVIE PKTGRKLAKL  60
   61 ECLDSRGFFS GVIPRRKNFF RYQLAVVWHG QQNLIDDPYR FGPLIQEMDA WLLSEG

[Run NCBI BLAST on this sequence.]

Detection Method:	FFAS03
Confidence:	2.39
Match:	1m7xA
Description:	1,4-alpha-glucan branching enzyme, N-terminal domain N; 1,4-alpha-glucan branching enzyme; 1,4-alpha-glucan branching enzyme, central domain
Matching Structure (courtesy of the PDB):

Predicted Domain #2

Region A:
Residues: [117-728]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 THLRPYETLG AHADTMDGVT GTRFSVWAPN ARRVSVVGQF NYWDGRRHPM RLRKESGIWE  60
   61 LFIPGAHNGQ LYKYEMIDAN GNLRLKSDPY AFEAQMRPET ASLICGLPEK VVQTEERKKA 120
  121 NQFDAPISIY EVHLGSWRRH TDNNFWLSYR ELADQLVPYA KWMGFTHLEL LPINEHPFDG 180
  181 SWGYQPTGLY APTRRFGTRD DFRYFIDAAH AAGLNVILDW VPGHFPTDDF ALAEFDGTNL 240
  241 YEHSDPREGY HQDWNTLIYN YGRREVSNFL VGNALYWIER FGIDALRVDA VASMIYRDYS 300
  301 RKEGEWIPNE FGGRENLEAI EFLRNTNRIL GEQVSGAVTM AEESTDFPGV SRPQDMGGLG 360
  361 FWYKWNLGWM HDTLDYMKLD PVYRQYHHDK LTFGILYNYT ENFVLPLSHD EVVHGKKSIL 420
  421 DRMPGDAWQK FANLRAYYGW MWAFPGKKLL FMGNEFAQGR EWNHDASLDW HLLEGGDNWH 480
  481 HGVQRLVRDL NLTYRHHKAM HELDFDPYGF EWLVVDDKER SVLIFVRRDK EGNEIIVASN 540
  541 FTPVPRHDYR FGINQPGKWR EILNTDSMHY HGSNAGNGGT VHSDEIASHG RQHSLSLTLP 600
  601 PLATIWLVRE AE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	157.0
Match:	1m7xA
Description:	1,4-alpha-glucan branching enzyme, N-terminal domain N; 1,4-alpha-glucan branching enzyme; 1,4-alpha-glucan branching enzyme, central domain
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
binding	1.81707419439683	bayes_pls_golite062009
protein binding	1.40303524581236	bayes_pls_golite062009
1,4-alpha-glucan branching enzyme activity	1.3556113866941	bayes_pls_golite062009
phosphorylase activity	0.99159540154441	bayes_pls_golite062009
glucosidase activity	0.766503513427695	bayes_pls_golite062009
hexosaminidase activity	0.763360027922833	bayes_pls_golite062009
cation transmembrane transporter activity	0.687079660016821	bayes_pls_golite062009
sugar binding	0.52048704356718	bayes_pls_golite062009
chitinase activity	0.500233744020165	bayes_pls_golite062009
ion transmembrane transporter activity	0.498330151821515	bayes_pls_golite062009
transferase activity, transferring hexosyl groups	0.266938437560648	bayes_pls_golite062009
beta-N-acetylhexosaminidase activity	0.263065978664289	bayes_pls_golite062009
ligand-gated ion channel activity	0.0325030313870961	bayes_pls_golite062009
ligand-gated channel activity	0.0325030313870961	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: