YRC Public Data Repository - Protein Structure Prediction

Protein:	BTUB_ECOLI
Organism:	Escherichia coli
Length:	614 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

[What does the above image mean?]

[Show Ginzu Version Information]

Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for BTUB_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|62130222, gi\|... - gi\|62182589\|ref\|YP_219006.1\| vitamin B12/cobalamin outer membrane transporter [Salmonella enterica s...	381.0	[0..1]	[614..15]
gi\|14252934 - gi\|14252934\|emb\|CAC39289.1\| hypothetical protein [Escherichia coli]	381.0	[0..1]	[614..53]
gi\|91213518, gi\|... - gi\|91213518\|ref\|YP_543504.1\| vitamin B12/cobalamin outer membrane transporter [Escherichia coli UTI8...	379.0	[0..1]	[614..16]
gi\|110617373, gi... - gi\|110807825\|ref\|YP_691345.1\| vitamin B12/cobalamin outer membrane transporter [Shigella flexneri 5 ...	379.0	[0..1]	[614..16]
gi\|226902944, gi... - gi\|237703007\|ref\|ZP_04533488.1\| vitamin B12/cobalamin outer membrane transporter [Escherichia sp. 3_... gi\|115515363, gi... - gi\|117626239\|ref\|YP_859562.1\| vitamin B12/cobalamin outer membrane transporter [Escherichia coli APE...	378.0	[0..1]	[614..15]
gi\|110644310, gi... - gi\|110644310\|ref\|YP_672040.1\| vitamin B12/cobalamin outer membrane transporter [Escherichia coli 536...	378.0	[0..1]	[614..15]
gi\|26111174, gi\|... - gi\|26250743\|ref\|NP_756783.1\| vitamin B12/cobalamin outer membrane transporter [Escherichia coli CFT0...	378.0	[0..1]	[614..16]
BTUB_SALPA - Vitamin B12 transporter BtuB OS=Salmonella paratyphi A GN=btuB PE=3 SV=1 gi\|205323764, gi... - gi\|205323764\|gb\|EDZ11603.1\| TonB-dependent vitamin B12 receptor [Salmonella enterica subsp. enterica... BTUB_SALCH - Vitamin B12 transporter BtuB OS=Salmonella choleraesuis (strain SC-B67) GN=btuB PE=3 SV=2 BTUB_SALPA - Vitamin B12 transporter BtuB OS=Salmonella paratyphi A (strain ATCC 9150 / SARB42) GN=btuB PE=3 SV=1 gi\|205341846, gi... - gi\|205341846\|gb\|EDZ28610.1\| TonB-dependent vitamin B12 receptor [Salmonella enterica subsp. enterica... gi\|168262958, gi... - gi\|205348100\|gb\|EDZ34731.1\| TonB-dependent vitamin B12 receptor [Salmonella enterica subsp. enterica... BTUB_SALCH - Vitamin B12 transporter btuB OS=Salmonella choleraesuis GN=btuB PE=3 SV=2 BTUB_SALCH - Vitamin B12 transporter BtuB OS=Salmonella choleraesuis GN=btuB PE=3 SV=2 gi\|197364894, gi... - gi\|197364894\|ref\|YP_002144531.1\| vitamin B12/cobalamin outer membrane transporter [Salmonella enteri... gi\|197249135, gi... - gi\|197249135\|ref\|YP_002149051.1\| TonB-dependent vitamin B12 receptor [Salmonella enterica subsp. ent... gi\|224470471, gi... - gi\|224585943\|ref\|YP_002639742.1\| vitamin B12 receptor protein [Salmonella enterica subsp. enterica s... gi\|200388778, gi... - gi\|200388778\|ref\|ZP_03215390.1\| TonB-dependent vitamin B12 receptor [Salmonella enterica subsp. ente...	376.0	[0..1]	[614..1]
gi\|16762313 - gi\|16762313\|ref\|NP_457930.1\| vitamin B12/cobalamin outer membrane transporter [Salmonella enterica s... BTUB_SALTI - Vitamin B12 transporter btuB OS=Salmonella typhi GN=btuB PE=3 SV=1 BTUB_SALTI - Vitamin B12 transporter BtuB OS=Salmonella typhi GN=btuB PE=3 SV=1 gi\|214003425 - gi\|214003425\|ref\|ZP_03414160.1\| vitamin B12/cobalamin outer membrane transporter [Salmonella enteric... gi\|29143801, gi\|... - gi\|29143801\|ref\|NP_807143.1\| vitamin B12/cobalamin outer membrane transporter [Salmonella enterica s... gi\|213427063 - gi\|213427063\|ref\|ZP_03359813.1\| vitamin B12/cobalamin outer membrane transporter [Salmonella enteric... gi\|213051819 - gi\|213051819\|ref\|ZP_03344697.1\| vitamin B12/cobalamin outer membrane transporter [Salmonella enteric...	375.0	[0..1]	[614..1]
gi\|15834151, gi\|... - gi\|15834151\|ref\|NP_312924.1\| vitamin B12/cobalamin outer membrane transporter [Escherichia coli O157... gi\|12518893, gi\|... - gi\|15804564\|ref\|NP_290605.1\| vitamin B12/cobalamin outer membrane transporter [Escherichia coli O157... gi\|208742033, gi... - gi\|208821910\|ref\|ZP_03262230.1\| TonB-dependent vitamin B12 receptor [Escherichia coli O157:H7 str. E... gi\|208734178, gi... - gi\|208812901\|ref\|ZP_03254230.1\| TonB-dependent vitamin B12 receptor [Escherichia coli O157:H7 str. E... gi\|208807737, gi... - gi\|208807737\|ref\|ZP_03250074.1\| TonB-dependent vitamin B12 receptor [Escherichia coli O157:H7 str. E... gi\|195937618 - gi\|195937618\|ref\|ZP_03083000.1\| vitamin B12/cobalamin outer membrane transporter [Escherichia coli O... gi\|217325058, gi... - gi\|217325058\|ref\|ZP_03441142.1\| TonB-dependent vitamin B12 receptor [Escherichia coli O157:H7 str. T... gi\|209159463, gi... - gi\|209398063\|ref\|YP_002273490.1\| TonB-dependent vitamin B12 receptor [Escherichia coli O157:H7 str. ... gi\|168761640, gi... - gi\|189367859\|gb\|EDU86275.1\| TonB-dependent vitamin B12 receptor [Escherichia coli O157:H7 str. EC450... gi\|188016818, gi... - gi\|188016818\|gb\|EDU54940.1\| TonB-dependent vitamin B12 receptor [Escherichia coli O157:H7 str. EC411... gi\|254595373, gi... - gi\|254795973\|ref\|YP_003080810.1\| vitamin B12/cobalamin outer membrane transporter [Escherichia coli ... gi\|168799441, gi... - gi\|189378007\|gb\|EDU96423.1\| TonB-dependent vitamin B12 receptor [Escherichia coli O157:H7 str. EC508... gi\|168786834, gi... - gi\|189373427\|gb\|EDU91843.1\| TonB-dependent vitamin B12 receptor [Escherichia coli O157:H7 str. EC869... BTUB_ECO57 - Vitamin B12 transporter BtuB OS=Escherichia coli O157:H7 GN=btuB PE=3 SV=1 gi\|168780895, gi... - gi\|189001505\|gb\|EDU70491.1\| TonB-dependent vitamin B12 receptor [Escherichia coli O157:H7 str. EC407... gi\|209751928, gi... - pir\|\|F86088 hypothetical protein btuB [imported] - Escherichia coli (strain O157:H7, substrain EDL9... gi\|187769150, gi... - gi\|187769150\|gb\|EDU32994.1\| TonB-dependent vitamin B12 receptor [Escherichia coli O157:H7 str. EC419...	374.0	[0..1]	[614..1]

Back

Predicted Domain #1

Region A:
Residues: [1-614]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MIKKASLLTA CSVTAFSAWA QDTSPDTLVV TANRFEQPRS TVLAPTTVVT RQDIDRWQST  60
   61 SVNDVLRRLP GVDITQNGGS GQLSSIFIRG TNASHVLVLI DGVRLNLAGV SGSADLSQFP 120
  121 IALVQRVEYI RGPRSAVYGS DAIGGVVNII TTRDEPGTEI SAGWGSNSYQ NYDVSTQQQL 180
  181 GDKTRVTLLG DYAHTHGYDV VAYGNTGTQA QTDNDGFLSK TLYGALEHNF TDAWSGFVRG 240
  241 YGYDNRTNYD AYYSPGSPLL DTRKLYSQSW DAGLRYNGEL IKSQLITSYS HSKDYNYDPH 300
  301 YGRYDSSATL DEMKQYTVQW ANNVIVGHGS IGAGVDWQKQ TTTPGTGYVE DGYDQRNTGI 360
  361 YLTGLQQVGD FTFEGAARSD DNSQFGRHGT WQTSAGWEFI EGYRFIASYG TSYKAPNLGQ 420
  421 LYGFYGNPNL DPEKSKQWEG AFEGLTAGVN WRISGYRNDV SDLIDYDDHT LKYYNEGKAR 480
  481 IKGVEATANF DTGPLTHTVS YDYVDARNAI TDTPLLRRAK QQVKYQLDWQ LYDFDWGITY 540
  541 QYLGTRYDKD YSSYPYQTVK MGGVSLWDLA VAYPVTSHLT VRGKIANLFD KDYETVYGYQ 600
  601 TAGREYTLSG SYTF

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	97.0
Match:	1nqfA
Description:	Outer membrane cobalamin transporter BtuB
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
transporter activity	4.15398171369398	bayes_pls_golite062009
transition metal ion transmembrane transporter activity	3.71720155502729	bayes_pls_golite062009
metal ion transmembrane transporter activity	3.51478170290895	bayes_pls_golite062009
cation transmembrane transporter activity	3.41811856763345	bayes_pls_golite062009
ion transmembrane transporter activity	3.35817595849318	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	3.29933669641504	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	2.88478677770584	bayes_pls_golite062009
iron ion transmembrane transporter activity	2.78006545332604	bayes_pls_golite062009
di-, tri-valent inorganic cation transmembrane transporter activity	2.64234123687059	bayes_pls_golite062009
substrate-specific transporter activity	2.43332676180972	bayes_pls_golite062009
transmembrane transporter activity	1.99898520864149	bayes_pls_golite062009
cobalamin binding	0.937576452004179	bayes_pls_golite062009
binding	0.895613726661701	bayes_pls_golite062009
channel activity	0.491712690153616	bayes_pls_golite062009
passive transmembrane transporter activity	0.491712690153616	bayes_pls_golite062009
iron ion binding	0.248363677949459	bayes_pls_golite062009
protein binding	0.230368843489798	bayes_pls_golite062009
lipid transporter activity	0.10911346172326	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: