YRC Public Data Repository - Protein Structure Prediction

Protein:	BIOF_ECOLI
Organism:	Escherichia coli
Length:	384 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for BIOF_ECOLI.

Description	E-value	Query Range	Subject Range
BIOF_THEPX - 8-amino-7-oxononanoate synthase OS=Thermoanaerobacter sp. (strain X514) GN=Teth514_0779 PE=3 SV=1 gi\|242258126, gi... - gi\|242258126\|ref\|ZP_04802958.1\| pyridoxal phosphate-dependent acyltransferase [Thermoanaerobacter sp...	346.0	[0..4]	[384..7]
gi\|241884087, gi... - gi\|241903572\|ref\|ZP_04788294.1\| pyridoxal phosphate-dependent acyltransferase [Thermoanaerobacter br... BIOF_THEP3 - 8-amino-7-oxononanoate synthase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) GN=...	345.0	[0..4]	[384..7]
gi\|85695191, gi\|... - gi\|85710787\|ref\|ZP_01041848.1\| 2-amino-3-ketobutyrate coenzyme A ligase [Idiomarina baltica OS145], ...	344.0	[0..2]	[384..4]
BIOF_SYMTH - 8-amino-7-oxononanoate synthase OS=Symbiobacterium thermophilum (strain T / IAM 14863) GN=STH1872 PE... BIKB_SYMTH - Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase OS=Symbiobacterium...	341.0	[0..1]	[384..1]
gi\|154357033, gi... - gi\|94492478\|ref\|ZP_01299685.1\| hypothetical protein CburD_01000055 [Coxiella burnetii Dugway 7E9-12]... gi\|95927705, gi\|... - gi\|95927705\|ref\|ZP_01310459.1\| hypothetical protein CburR_01000469 [Coxiella burnetii RSA 331], gi\|1... gi\|165917892, gi... - gi\|165918386\|ref\|ZP_02218472.1\| 2-amino-3-ketobutyrate coenzyme A ligase [Coxiella burnetii RSA 334]... gi\|212011768, gi... - gi\|212213358\|ref\|YP_002304294.1\| 2-amino-3-ketobutyrate coenzyme A ligase [Coxiella burnetii CbuG_Q2... gi\|29653469, gi\|... - gi\|29653469\|ref\|NP_819161.1\| 2-amino-3-ketobutyrate coenzyme A ligase [Coxiella burnetii RSA 493], g...	341.0	[0..4]	[384..5]
gi\|54261520 - gi\|54261520\|gb\|AAH84616.1\| LOC495283 protein [Xenopus laevis]	339.0	[0..3]	[383..157]
HEM0_OPSTA - 5-aminolevulinate synthase, erythroid-specific, mitochondrial OS=Opsanus tau GN=alas2 PE=2 SV=1	339.0	[0..2]	[383..139]
HEM0_BOVIN - 5-aminolevulinate synthase, erythroid-specific, mitochondrial OS=Bos taurus GN=ALAS2 PE=2 SV=1	338.0	[0..3]	[383..145]
gi\|28586 - gi\|28586\|emb\|CAA39795.1\| delta-aminolevulinate synthase (erythroid) [Homo sapiens]	338.0	[0..2]	[380..139]
HEM0_DELLE - 5-aminolevulinate synthase, erythroid-specific, mitochondrial OS=Delphinapterus leucas GN=ALAS2 PE=2...	338.0	[0..3]	[383..140]

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Predicted Domain #1

Region A:
Residues: [1-384]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSWQEKINAA LDARRAADAL RRRYPVAQGA GRWLVADDRQ YLNFSSNDYL GLSHHPQIIR  60
   61 AWQQGAEQFG IGSGGSGHVS GYSVVHQALE EELAEWLGYS RALLFISGFA ANQAVIAAMM 120
  121 AKEDRIAADR LSHASLLEAA SLSPSQLRRF AHNDVTHLAR LLASPCPGQQ MVVTEGVFSM 180
  181 DGDSAPLAEI QQVTQQHNGW LMVDDAHGTG VIGEQGRGSC WLQKVKPELL VVTFGKGFGV 240
  241 SGAAVLCSST VADYLLQFAR HLIYSTSMPP AQAQALRASL AVIRSDEGDA RREKLAALIT 300
  301 RFRAGVQDLP FTLADSCSAI QPLIVGDNSR ALQLAEKLRQ QGCWVTAIRP PTVPAGTARL 360
  361 RLTLTAAHEM QDIDRLLEVL HGNG

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	84.522879
Match:	1dj9A
Description:	PLP-dependent acyl-CoA synthase (8-amino-7-oxonanoate synthase, AONS)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
catalytic activity	2.66922865456094	bayes_pls_golite062009
threonine aldolase activity	1.96256164426812	bayes_pls_golite062009
transferase activity, transferring nitrogenous groups	1.85532625717753	bayes_pls_golite062009
transaminase activity	1.83482013760414	bayes_pls_golite062009
adenosylmethionine-8-amino-7-oxononanoate transaminase activity	1.8294165617831	bayes_pls_golite062009
8-amino-7-oxononanoate synthase activity	1.68758133244562	bayes_pls_golite062009
L-aspartate:2-oxoglutarate aminotransferase activity	1.48194418319516	bayes_pls_golite062009
5-aminolevulinate synthase activity	1.44455472154869	bayes_pls_golite062009
carbon-sulfur lyase activity	1.19843805346966	bayes_pls_golite062009
transferase activity	0.93810038069762	bayes_pls_golite062009
intramolecular transferase activity, transferring amino groups	0.83247653897701	bayes_pls_golite062009
N-succinyltransferase activity	0.73312481571279	bayes_pls_golite062009
glycine dehydrogenase (decarboxylating) activity	0.520614909745139	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors, disulfide as acceptor	0.520614909745139	bayes_pls_golite062009
cystathionine beta-lyase activity	0.31229842545297	bayes_pls_golite062009
glycine C-acetyltransferase activity	0.20784545328672	bayes_pls_golite062009
glutamate-1-semialdehyde 2,1-aminomutase activity	0.0464359482474901	bayes_pls_golite062009
lyase activity	0.0414385380659299	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: