YRC Public Data Repository - Protein Structure Prediction

Protein:	BGLR_ECOLI
Organism:	Escherichia coli
Length:	603 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for BGLR_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|22094847 - gi\|22094847\|gb\|AAM92012.1\|AF527487_1 beta-glucuronidase-interferon gamma fusion protein; GUSIFN-g fu...	515.0	[0..1]	[603..13]
gi\|90992872 - gi\|90992872\|gb\|ABE03625.1\| GUS-eGFP fusion protein [Binary vector pGPro1] gi\|157781200 - gi\|157781200\|gb\|ABV71994.1\| gusA-eGFP [Binary vector pGPro2]	514.0	[0..2]	[603..7]
gi\|7638085 - gi\|7638085\|gb\|AAF65352.1\| mGFP5-GUSA fusion protein [Binary vector pCAMBIA-1304] gi\|118402822 - gi\|118402822\|emb\|CAJ44417.1\| fusion protein [Cloning vector pUDSC-B]	511.0	[0..2]	[603..245]
gi\|7638079 - gi\|7638079\|gb\|AAF65347.1\| GUSA (N358Q)/MGFP5 fusion, hexaHis tagged [Binary vector pCAMBIA-1303]	511.0	[0..2]	[603..7]
gi\|210076752 - gi\|210076752\|gb\|ACJ06731.1\| mGFP/GUS [Cloning vector pRGK374] gi\|67772470 - gi\|67772470\|gb\|AAY81662.1\| mGFP/GUS [Cloning vector pRGK337]	511.0	[0..2]	[603..262]
gi\|16209179 - gi\|16209179\|gb\|AAL09914.1\| phoB-lacZ-uidA2(pr) [CRIM plasmid pCAH63] gi\|16209061 - gi\|16209061\|gb\|AAL09841.1\| phoB-lacZ-uidA2(pr) [CRIM plasmid pAH55] gi\|16209067 - gi\|16209067\|gb\|AAL09845.1\| phoB-lacZ-uidA2(pr) [CRIM plasmid pAH56] gi\|16209174 - gi\|16209174\|gb\|AAL09910.1\| phoB-lacZ-uidA2(pr) [CRIM plasmid pCAH56]	505.0	[0..1]	[603..97]
gi\|75238822, gi\|... - gi\|75238822\|ref\|ZP_00722806.1\| COG3250: Beta-galactosidase/beta-glucuronidase [Escherichia coli F11]... gi\|110641739, gi... - gi\|110641739\|ref\|YP_669469.1\| beta-D-glucuronidase [Escherichia coli 536], gi\|110343331\|gb\|ABG69568....	504.0	[0..1]	[603..1]
gi\|110805590, gi... - gi\|110805590\|ref\|YP_689110.1\| beta-D-glucuronidase [Shigella flexneri 5 str. 8401], gi\|110615138\|gb\|...	503.0	[0..1]	[603..1]

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Predicted Domain #1

Region A:
Residues: [1-603]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLRPVETPTR EIKKLDGLWA FSLDRENCGI DQRWWESALQ ESRAIAVPGS FNDQFADADI  60
   61 RNYAGNVWYQ REVFIPKGWA GQRIVLRFDA VTHYGKVWVN NQEVMEHQGG YTPFEADVTP 120
  121 YVIAGKSVRI TVCVNNELNW QTIPPGMVIT DENGKKKQSY FHDFFNYAGI HRSVMLYTTP 180
  181 NTWVDDITVV THVAQDCNHA SVDWQVVANG DVSVELRDAD QQVVATGQGT SGTLQVVNPH 240
  241 LWQPGEGYLY ELCVTAKSQT ECDIYPLRVG IRSVAVKGEQ FLINHKPFYF TGFGRHEDAD 300
  301 LRGKGFDNVL MVHDHALMDW IGANSYRTSH YPYAEEMLDW ADEHGIVVID ETAAVGFNLS 360
  361 LGIGFEAGNK PKELYSEEAV NGETQQAHLQ AIKELIARDK NHPSVVMWSI ANEPDTRPQG 420
  421 AREYFAPLAE ATRKLDPTRP ITCVNVMFCD AHTDTISDLF DVLCLNRYYG WYVQSGDLET 480
  481 AEKVLEKELL AWQEKLHQPI IITEYGVDTL AGLHSMYTDM WSEEYQCAWL DMYHRVFDRV 540
  541 SAVVGEQVWN FADFATSQGI LRVGGNKKGI FTRDRKPKSA AFLLQKRWTG MNFGEKPQQG 600
  601 GKQ

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	116.0
Match:	1bhgA
Description:	beta-Glucuronidase; beta-Glucuronidase, domain 3
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
hydrolase activity, hydrolyzing O-glycosyl compounds	6.26329339735894	bayes_pls_golite062009
hydrolase activity, acting on glycosyl bonds	5.92180849080637	bayes_pls_golite062009
hydrolase activity	3.98075336331567	bayes_pls_golite062009
hexosaminidase activity	3.21852337137039	bayes_pls_golite062009
beta-N-acetylhexosaminidase activity	2.69072465526737	bayes_pls_golite062009
hyalurononglucosaminidase activity	2.33846657668199	bayes_pls_golite062009
catalytic activity	1.85569074294201	bayes_pls_golite062009
glucosidase activity	1.85545420386612	bayes_pls_golite062009
galactosidase activity	1.26155841119987	bayes_pls_golite062009
alpha-glucosidase activity	1.00973231642658	bayes_pls_golite062009
peptidase activity	0.618122797487524	bayes_pls_golite062009
binding	0.601963252939415	bayes_pls_golite062009
beta-glucuronidase activity	0.55376725638834	bayes_pls_golite062009
sugar binding	0.52048704356718	bayes_pls_golite062009
mannosidase activity	0.48536369022293	bayes_pls_golite062009
peptidase activity, acting on L-amino acid peptides	0.340224150705178	bayes_pls_golite062009
carbohydrate binding	0.31275257729319	bayes_pls_golite062009
transferase activity, transferring hexosyl groups	0.266938437560648	bayes_pls_golite062009
fucose binding	0.0946432585122601	bayes_pls_golite062009
beta-galactosidase activity	0.0137149865738109	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: