YRC Public Data Repository - Protein Structure Prediction

Protein:	ADA_ECOLI
Organism:	Escherichia coli
Length:	354 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for ADA_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|75243029, gi\|... - gi\|75243029\|ref\|ZP_00726735.1\| COG2169: Adenosine deaminase [Escherichia coli F11], gi\|191169958\|ref... gi\|110344015, gi... - gi\|110642423\|ref\|YP_670153.1\| ADA regulatory protein [Escherichia coli 536], gi\|110344015\|gb\|ABG7025...	307.0	[0..1]	[354..1]
gi\|253974126, gi... - gi\|254162225\|ref\|YP_003045333.1\| fused DNA-binding transcriptional dual regulator/O6-methylguanine-D... gi\|194424888, gi... - gi\|194436160\|ref\|ZP_03068262.1\| regulatory protein Ada [Escherichia coli 101-1], gi\|194424888\|gb\|EDX... gi\|145191 - gi\|145191\|gb\|AAA23413.1\| Ada polyprotein gi\|253978293, gi... - gi\|254288986\|ref\|YP_003054734.1\| fused DNA-binding transcriptional dual regulator/O6-methylguanine-D... gi\|242377849, gi... - gi\|251785576\|ref\|YP_002999880.1\| hypothetical protein B21_02099 [Escherichia coli BL21], gi\|24237784...	306.0	[0..1]	[354..1]
gi\|24052652, gi\|... - gi\|24113598\|ref\|NP_708108.1\| O6-methylguanine-DNA methyltransferase; transcription activator/repress... gi\|81245964, gi\|... - gi\|82544553\|ref\|YP_408500.1\| O6-methylguanine-DNA methyltransferase; transcription activator/repress... gi\|73856211, gi\|... - gi\|74312734\|ref\|YP_311153.1\| O6-methylguanine-DNA methyltransferase [Shigella sonnei Ss046], gi\|7385... gi\|110806187, gi... - gi\|110806187\|ref\|YP_689707.1\| O6-methylguanine-DNA methyltransferase; transcription activator/repres... gi\|30063652, gi\|... - gi\|30063652\|ref\|NP_837823.1\| O6-methylguanine-DNA methyltransferase; transcription activator/repress...	305.0	[0..1]	[354..1]
gi\|227887271, gi... - gi\|227887271\|ref\|ZP_04005076.1\| O6-methylguanine-DNA methyltransferase; transcription activator/repr... gi\|26248600, gi\|... - gi\|26248600\|ref\|NP_754640.1\| ADA regulatory protein [Escherichia coli CFT073], gi\|26109005\|gb\|AAN812...	305.0	[0..1]	[348..1]
gi\|187429131, gi... - gi\|75176156\|ref\|ZP_00696314.1\| COG2169: Adenosine deaminase [Shigella boydii BS512], gi\|187732139\|re...	304.0	[0..1]	[354..1]
gi\|13362571, gi\|... - gi\|15832356\|ref\|NP_311129.1\| O6-methylguanine-DNA methyltransferase [Escherichia coli O157:H7], gi\|1... gi\|25286155, gi\|... - pir\|\|H85860 O6-methylguanine-DNA methyltransferase [imported] - Escherichia coli (strain O157:H7, su... gi\|15802766, gi\|... - gi\|15802766\|ref\|NP_288793.1\| O6-methylguanine-DNA methyltransferase; transcription activator/repress... gi\|13362571, gi\|... - gi\|15832356\|ref\|NP_311129.1\| O6-methylguanine-DNA methyltransferase [Escherichia coli O157:H7 str. S... gi\|208818942, gi... - gi\|208818942\|ref\|ZP_03259262.1\| regulatory protein Ada [Escherichia coli O157:H7 str. EC4042], gi\|20... gi\|208813133, gi... - gi\|208813133\|ref\|ZP_03254462.1\| regulatory protein Ada [Escherichia coli O157:H7 str. EC4045], gi\|20... gi\|217326795, gi... - gi\|217326795\|ref\|ZP_03442878.1\| regulatory protein Ada [Escherichia coli O157:H7 str. TW14588], gi\|2... gi\|209398796, gi... - gi\|209398796\|ref\|YP_002271626.1\| regulatory protein Ada [Escherichia coli O157:H7 str. EC4115], gi\|2... gi\|168803003, gi... - gi\|189375218\|gb\|EDU93634.1\| regulatory protein Ada [Escherichia coli O157:H7 str. EC508], gi\|1688030... gi\|189372959, gi... - gi\|189372959\|gb\|EDU91375.1\| regulatory protein Ada [Escherichia coli O157:H7 str. EC869], gi\|1687871... gi\|254794112, gi... - gi\|254794112\|ref\|YP_003078949.1\| fused DNA-binding transcriptional dual regulator, O6-methylguanine-... gi\|208809181, gi... - gi\|208809181\|ref\|ZP_03251518.1\| regulatory protein Ada [Escherichia coli O157:H7 str. EC4206], gi\|20... gi\|195935594 - gi\|195935594\|ref\|ZP_03080976.1\| O6-methylguanine-DNA methyltransferase [Escherichia coli O157:H7 str... gi\|168767837, gi... - gi\|189363020\|gb\|EDU81439.1\| regulatory protein Ada [Escherichia coli O157:H7 str. EC4486], gi\|168767... gi\|189365353, gi... - gi\|189365353\|gb\|EDU83769.1\| regulatory protein Ada [Escherichia coli O157:H7 str. EC4501], gi\|168764... gi\|189002004, gi... - gi\|189002004\|gb\|EDU70990.1\| regulatory protein Ada [Escherichia coli O157:H7 str. EC4076], gi\|168780... gi\|187771050, gi... - gi\|187771050\|gb\|EDU34894.1\| regulatory protein Ada [Escherichia coli O157:H7 str. EC4196], gi\|168773... gi\|168754964, gi... - gi\|189357736\|gb\|EDU76155.1\| regulatory protein Ada [Escherichia coli O157:H7 str. EC4401], gi\|168754... gi\|168749620, gi... - gi\|188016039\|gb\|EDU54161.1\| regulatory protein Ada [Escherichia coli O157:H7 str. EC4113], gi\|168749...	303.0	[0..1]	[354..1]
gi\|226901054, gi... - gi\|237704688\|ref\|ZP_04535169.1\| O6-methylguanine-DNA methyltransferase [Escherichia sp. 3_2_53FAA], ... gi\|218365900, gi... - gi\|218559131\|ref\|YP_002392044.1\| fused DNA-binding transcriptional dual regulator ; O6-methylguanine... gi\|117624407, gi... - gi\|117624407\|ref\|YP_853320.1\| Ada transcriptional dual regulator / O-6-methylguanine-DNA methyltrans... gi\|91211505, gi\|... - gi\|91211505\|ref\|YP_541491.1\| Ada transcriptional dual regulator / O-6-methylguanine-DNA methyltransf...	302.0	[0..1]	[354..1]
gi\|82776183, gi\|... - gi\|82776183\|ref\|YP_402532.1\| O6-methylguanine-DNA methyltransferase [Shigella dysenteriae Sd197], gi...	302.0	[0..1]	[354..1]
gi\|75231449 - gi\|75231449\|ref\|ZP_00717818.1\| COG2169: Adenosine deaminase [Escherichia coli B7A] gi\|157078603, gi... - gi\|75187009\|ref\|ZP_00700276.1\| COG2169: Adenosine deaminase [Escherichia coli E24377A], gi\|157156573...	301.0	[0..1]	[354..1]

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Predicted Domain #1

Region A:
Residues: [1-174]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKKATCLTDD QRWQSVLARD PNADGEFVFA VRTTGIFCRP SCRARHALRE NVSFYANASE  60
   61 ALAAGFRPCK RCQPEKANAQ QHRLDKITHA CRLLEQETPV TLEALADQVA MSPFHLHRLF 120
  121 KATTGMTPKA WQQAWRARRL RESLAKGESV TTSILNAGFP DSSSYYRKAD ETLG

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	33.522879
Match:	1wpkA
Description:	Methylated Form of N-terminal Transcriptional Regulator Domain of Escherichia Coli Ada Protein
Matching Structure (courtesy of the PDB):

Predicted Domain #2

Region A:
Residues: [175-354]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTAKQFRHGG ENLAVRYALA DCELGRCLVA ESERGICAIL LGDDDATLIS ELQQMFPAAD  60
   61 NAPADLMFQQ HVREVIASLN QRDTPLTLPL DIRGTAFQQQ VWQALRTIPC GETVSYQQLA 120
  121 NAIGKPKAVR AVASACAANK LAIIIPCHRV VRGDGTLSGY RWGVSRKAQL LRREAENEER 180
  181

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	34.69897
Match:	1sfeA
Description:	ADA O6-METHYLGUANINE-DNA METHYLTRANSFERASE FROM ESCHERICHIA COLI
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
methylated-DNA-[protein]-cysteine S-methyltransferase activity	3.81641972619245	bayes_pls_golite062009
transcription regulator activity	3.06900146562384	bayes_pls_golite062009
DNA binding	2.96596668208419	bayes_pls_golite062009
nucleic acid binding	2.92705798633591	bayes_pls_golite062009
S-methyltransferase activity	2.66007025371147	bayes_pls_golite062009
transcription factor activity	2.45682731123393	bayes_pls_golite062009
catalytic activity	1.90838528948382	bayes_pls_golite062009
binding	1.9004221787372	bayes_pls_golite062009
transferase activity	1.22815682532854	bayes_pls_golite062009
methyltransferase activity	1.14625481859451	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	1.13184530961695	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: