YRC Public Data Repository - Protein Structure Prediction

Protein:	nfs1
Organism:	Schizosaccharomyces pombe
Length:	498 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for nfs1.

Description	E-value	Query Range	Subject Range
NFS1_ASHGO - Cysteine desulfurase, mitochondrial OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 /... NFS1_ASHGO - Cysteine desulfurase, mitochondrial OS=Ashbya gossypii GN=NFS1 PE=3 SV=1	342.0	[0..15]	[498..6]
gi\|119191496, gi... - gi\|90305140\|gb\|EAS34771.1\| conserved hypothetical protein [Coccidioides immitis RS], gi\|119191496\|re...	342.0	[0..2]	[498..6]
gi\|85092004 - gi\|85092004\|ref\|XP_959179.1\| cysteine desulfurase, mitochondrial precursor [Neurospora crassa OR74A] gi\|28920581, gi\|... - gi\|32406758\|ref\|XP_323992.1\| hypothetical protein [Neurospora crassa], gi\|28920581\|gb\|EAA29943.1\| cy...	337.0	[0..20]	[498..12]
gi\|115390312, gi... - gi\|115390312\|ref\|XP_001212661.1\| cysteine desulfurase, mitochondrial precursor [Aspergillus terreus ...	337.0	[0..8]	[498..10]
gi\|194419577, gi... - gi\|83570750\|ref\|ZP_00922193.1\| COG1104: Cysteine sulfinate desulfinase/cysteine desulfurase and rela...	336.0	[0..102]	[498..6]
ISCS_SHIFL - Cysteine desulfurase IscS OS=Shigella flexneri GN=iscS PE=3 SV=1 sp\|B7UGX6\|ISCS_ECO27 - Cysteine desulfurase OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=iscS PE=3 SV=1 EG12677 - cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent / Cysteine desulfurase, PLP-dependent; ... ISCS_ECOLI - Cysteine desulfurase (EC 2.8.1.7) (ThiI transpersulfidase) (NifS protein homolog) - Escherichia coli gi\|30042176, gi\|... - gi\|30063920\|ref\|NP_838091.1\| cysteine desulfurase [Shigella flexneri 2a str. 2457T], gi\|30042176\|gb\|... ISCS_ECO57 - Cysteine desulfurase IscS OS=Escherichia coli O157:H7 GN=iscS PE=1 SV=1 ISCS_ECOL6 - Cysteine desulfurase OS=Escherichia coli O6 GN=iscS PE=3 SV=1 gi\|75237724, gi\|... - gi\|75237724\|ref\|ZP_00721740.1\| COG1104: Cysteine sulfinate desulfinase/cysteine desulfurase and rela... gi\|190907079, gi... - gi\|75240445\|ref\|ZP_00724379.1\| COG1104: Cysteine sulfinate desulfinase/cysteine desulfurase and rela... EG12677 - cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent / Cysteine desulfurase, PLP-dependent; ... gi\|75257954, gi\|... - gi\|75257954\|ref\|ZP_00729428.1\| COG1104: Cysteine sulfinate desulfinase/cysteine desulfurase and rela... gi\|188490280, gi... - gi\|75515869\|ref\|ZP_00738004.1\| COG1104: Cysteine sulfinate desulfinase/cysteine desulfurase and rela... gi\|56383678, gi\|... - gi\|56480127\|ref\|NP_708369.2\| cysteine desulfurase [Shigella flexneri 2a str. 301], gi\|56383678\|gb\|AA... ISCS_ECOBW - Cysteine desulfurase IscS OS=Escherichia coli (strain K12 / MC4100 / BW2952) GN=iscS PE=3 SV=1 gi\|242378130, gi... - gi\|251785857\|ref\|YP_003000161.1\| hypothetical protein B21_02386 [Escherichia coli BL21], gi\|24237813... gi\|83586820, gi\|... - gi\|83586820\|ref\|ZP_00925451.1\| COG1104: Cysteine sulfinate desulfinase/cysteine desulfurase and rela... gi\|253978572, gi... - gi\|254289265\|ref\|YP_003055013.1\| cysteine desulfurase [Escherichia coli BL21(DE3)], gi\|253978572\|gb\|... ISCS_SHIB3 - Cysteine desulfurase IscS OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=iscS PE=3 S... gi\|254162504, gi... - gi\|254162504\|ref\|YP_003045612.1\| cysteine desulfurase [Escherichia coli B str. REL606], gi\|253974405... ISCS_ECO24 - Cysteine desulfurase IscS OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=iscS PE=3 SV=1 gi\|254593880, gi... - gi\|254794480\|ref\|YP_003079317.1\| cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent [Esche... ISCS_ECOHS - Cysteine desulfurase IscS OS=Escherichia coli O9:H4 (strain HS) GN=iscS PE=3 SV=1 gi\|208805832, gi... - gi\|208805832\|ref\|ZP_03248169.1\| cysteine desulfurase IscS [Escherichia coli O157:H7 str. EC4206], gi... gi\|195936676 - gi\|195936676\|ref\|ZP_03082058.1\| cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent [Escher... gi\|208819169, gi... - gi\|208819169\|ref\|ZP_03259489.1\| cysteine desulfurase IscS [Escherichia coli O157:H7 str. EC4042], gi... gi\|208813419, gi... - gi\|208813419\|ref\|ZP_03254748.1\| cysteine desulfurase IscS [Escherichia coli O157:H7 str. EC4045], gi... ISCS_ECOL5 - Cysteine desulfurase IscS OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=iscS PE=3 SV=1 ISCS_ECOSE - Cysteine desulfurase IscS OS=Escherichia coli (strain SE11) GN=iscS PE=3 SV=1 ISCS_SHIF8 - Cysteine desulfurase IscS OS=Shigella flexneri serotype 5b (strain 8401) GN=iscS PE=3 SV=1 ISCS_ECO5E - Cysteine desulfurase IscS OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=iscS PE=3 SV=1 gi\|217327829, gi... - gi\|217327829\|ref\|ZP_03443912.1\| cysteine desulfurase IscS [Escherichia coli O157:H7 str. TW14588], g... ISCS_ECO27 - Cysteine desulfurase IscS OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=iscS PE=3 SV=1 gi\|187770614, gi... - gi\|187770614\|gb\|EDU34458.1\| cysteine desulfurase IscS [Escherichia coli O157:H7 str. EC4196], gi\|168... gi\|168768628, gi... - gi\|189362199\|gb\|EDU80618.1\| cysteine desulfurase IscS [Escherichia coli O157:H7 str. EC4486], gi\|168... gi\|189372294, gi... - gi\|189372294\|gb\|EDU90710.1\| cysteine desulfurase IscS [Escherichia coli O157:H7 str. EC869], gi\|1687... gi\|168778502, gi... - gi\|189003342\|gb\|EDU72328.1\| cysteine desulfurase IscS [Escherichia coli O157:H7 str. EC4076], gi\|168... ISCS_ECODH - Cysteine desulfurase IscS OS=Escherichia coli (strain K12 / DH10B) GN=iscS PE=3 SV=1 gi\|189378660, gi... - gi\|189378660\|gb\|EDU97076.1\| cysteine desulfurase IscS [Escherichia coli O157:H7 str. EC508], gi\|1687... gi\|170123547, gi... - gi\|170767213\|ref\|ZP_02901666.1\| cysteine desulfurase IscS [Escherichia albertii TW07627], gi\|1701235... ISCS_ECOSM - Cysteine desulfurase IscS OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=iscS PE=3 SV=1 gi\|124499824, gi... - gi\|124529517\|ref\|ZP_01700487.1\| cysteine desulfurase IscS [Escherichia coli B], gi\|124499824\|gb\|EAY4... ISCS_ECO45 - Cysteine desulfurase IscS OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=iscS PE=3 SV=1 ISCS_ECO8A - Cysteine desulfurase IscS OS=Escherichia coli O8 (strain IAI1) GN=iscS PE=3 SV=1 ISCS_ECO55 - Cysteine desulfurase IscS OS=Escherichia coli (strain 55989 / EAEC) GN=iscS PE=3 SV=1 ISCS_ECO81 - Cysteine desulfurase IscS OS=Escherichia coli O81 (strain ED1a) GN=iscS PE=3 SV=1 ISCS_ECOLU - Cysteine desulfurase IscS OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN=iscS PE=3 SV=1 ISCS_ECO7I - Cysteine desulfurase IscS OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=iscS PE=3 SV=1 ISCS_ECOLC - Cysteine desulfurase IscS OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=iscS PE=3 SV... gi\|168748405, gi... - gi\|188017140\|gb\|EDU55262.1\| cysteine desulfurase IscS [Escherichia coli O157:H7 str. EC4113], gi\|168... gi\|189368372, gi... - gi\|189368372\|gb\|EDU86788.1\| cysteine desulfurase IscS [Escherichia coli O157:H7 str. EC4501], gi\|168... gi\|189356618, gi... - gi\|189356618\|gb\|EDU75037.1\| cysteine desulfurase IscS [Escherichia coli O157:H7 str. EC4401], gi\|168... ISCS_ECOL6 - Cysteine desulfurase IscS OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) GN=iscS PE=... gi\|1799938, gi\|8... - gi\|89109336\|ref\|AP_003116.1\| cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent [Escherich... gi\|38704100 - gi\|38704100\|ref\|NP_311423.2\| cysteine desulfurase [Escherichia coli O157:H7 str. Sakai]	336.0	[0..102]	[498..6]
gi\|70998961, gi\|... - gi\|70998961\|ref\|XP_754202.1\| tRNA splicing protein (Spl1) [Aspergillus fumigatus Af293], gi\|66851839... gi\|159127220 - gi\|159127220\|gb\|EDP52335.1\| tRNA splicing protein (Spl1), putative [Aspergillus fumigatus A1163]	336.0	[0..4]	[498..6]
gi\|194414604, gi... - gi\|75211496\|ref\|ZP_00711586.1\| COG1104: Cysteine sulfinate desulfinase/cysteine desulfurase and rela...	336.0	[0..102]	[498..6]
ISCS_SALPB - Cysteine desulfurase IscS OS=Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7) GN=iscS PE=3 SV=1 ISCS_SALEP - Cysteine desulfurase IscS OS=Salmonella enteritidis PT4 (strain P125109) GN=iscS PE=3 SV=1 ISCS_SALG2 - Cysteine desulfurase IscS OS=Salmonella gallinarum (strain 287/91 / NCTC 13346) GN=iscS PE=3 SV=1 gi\|204929449, gi... - gi\|204929449\|ref\|ZP_03220523.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serov... gi\|200389006, gi... - gi\|200389006\|ref\|ZP_03215618.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serov... ISCS_SALTY - Cysteine desulfurase OS=Salmonella typhimurium GN=iscS PE=3 SV=1 ISCS_SALCH - Cysteine desulfurase OS=Salmonella choleraesuis GN=iscS PE=3 SV=1 gi\|238912664 - gi\|238912664\|ref\|ZP_04656501.1\| cysteine desulfurase [Salmonella enterica subsp. enterica serovar Te... ISCS_SALTY - Cysteine desulfurase OS=Salmonella typhimurium GN=iscS PE=3 SV=1 ISCS_SALPA - Cysteine desulfurase IscS OS=Salmonella paratyphi A (strain ATCC 9150 / SARB42) GN=iscS PE=3 SV=1 lcl\|NC_016856.1_... - [gene=nifS] [protein=cysteine desulfurase] [protein_id=YP_005238645.1] [location=complement(2733089.... ISCS_SALPA - Cysteine desulfurase OS=Salmonella paratyphi A GN=iscS PE=3 SV=1 ISCS_SALCH - Cysteine desulfurase OS=Salmonella choleraesuis GN=iscS PE=3 SV=1 ISCS_SALCH - Cysteine desulfurase IscS OS=Salmonella choleraesuis (strain SC-B67) GN=iscS PE=3 SV=1 gi\|194471395, gi... - gi\|194471395\|ref\|ZP_03077379.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serov... ISCS_SALHS - Cysteine desulfurase IscS OS=Salmonella heidelberg (strain SL476) GN=iscS PE=3 SV=1 ISCS_SALNS - Cysteine desulfurase IscS OS=Salmonella newport (strain SL254) GN=iscS PE=3 SV=1 ISCS_SALTY - Cysteine desulfurase IscS OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) GN=iscS PE... gi\|168821477, gi... - gi\|205341918\|gb\|EDZ28682.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar W... ISCS_SALDC - Cysteine desulfurase IscS OS=Salmonella dublin (strain CT_02021853) GN=iscS PE=3 SV=1 ISCS_SALPK - Cysteine desulfurase IscS OS=Salmonella paratyphi A (strain AKU_12601) GN=iscS PE=3 SV=1 gi\|197263596, gi... - gi\|197263596\|ref\|ZP_03163670.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serov... ISCS_SALPB - Cysteine desulfurase OS=Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7) GN=iscS PE=3 SV=1 ISCS_SALA4 - Cysteine desulfurase IscS OS=Salmonella agona (strain SL483) GN=iscS PE=3 SV=1 gi\|167992668, gi... - gi\|205329088\|gb\|EDZ15852.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar 4... gi\|205325222, gi... - gi\|205325222\|gb\|EDZ13061.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar S... gi\|168466701, gi... - gi\|195630769\|gb\|EDX49361.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar N... gi\|168261415, gi... - gi\|205349590\|gb\|EDZ36221.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar H... gi\|168243327, gi... - gi\|205337629\|gb\|EDZ24393.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar H... gi\|205333600, gi... - gi\|205333600\|gb\|EDZ20364.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar K...	336.0	[0..102]	[498..6]
NFS1 - Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis; required for the post-transc...	336.0	[0..85]	[498..84]

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Predicted Domain #1

Region A:
Residues: [1-77]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGSLMLSRAR CLLELRSALF FVKRESQDIN NVRQSLGVYG RSFMTSSRMD KPSMSNKPRE  60
   61 QMYGLGNMTA VQEPIPE

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [78-498]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NSLKTVTLDQ AQTAASTVTG LHPIYMDFQA TSPLDYRVLD SMLPFFTGIY GNPHSRTHAY  60
   61 GWEAEKAVEN ARQEIASVIN ADPREIIFTS GATESNNAIL KGVARFYKSR KKHLVSVQTE 120
  121 HKCVLDSLRA LQEEGFEVTF LPVQTNGLIN LDELRDAIRP DTVCVSVMAV NNEIGVCQPL 180
  181 EEIGKICRQK KVFFHSDAAQ GYGKIDIDVN RMNIDLMSIS AHKIYGPKGI GAAYVRRRPR 240
  241 VRLEPLISGG GQERGLRSGT LAPSQVVGFG TAARICKEEM KYDYAHISKL SQRLIDGLLA 300
  301 IPYTSLNGDP KSRYPGCVNI SFNYVEGESL LMGLKNIALS SGSACTSASL EPSYVLRAIG 360
  361 QSDENAHSSI RFGIGRFTTE AEIDYAIENV SRQVSFLRNM SPLWDLVQEG VDLSTIEWSQ 420
  421 H

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	92.154902
Match:	1p3wB
Description:	Cysteine desulfurase IscS
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
oxidoreductase activity, acting on the CH-NH2 group of donors, disulfide as acceptor	3.40354781002256	bayes_pls_golite062009
glycine dehydrogenase (decarboxylating) activity	3.40354781002256	bayes_pls_golite062009
transferase activity	2.59075614411581	bayes_pls_golite062009
L-aspartate:2-oxoglutarate aminotransferase activity	2.30133284041702	bayes_pls_golite062009
catalytic activity	2.29878871754893	bayes_pls_golite062009
cysteine desulfurase activity	1.30923778688541	bayes_pls_golite062009
transferase activity, transferring nitrogenous groups	1.28316822767388	bayes_pls_golite062009
transaminase activity	1.24990480711889	bayes_pls_golite062009
L-alanine:2-oxoglutarate aminotransferase activity	1.06988190956423	bayes_pls_golite062009
alanine-oxo-acid transaminase activity	1.06988190956423	bayes_pls_golite062009
transcription regulator activity	0.977210827616757	bayes_pls_golite062009
binding	0.942651665520336	bayes_pls_golite062009
ligase activity	0.827063849687833	bayes_pls_golite062009
DNA binding	0.803965264969424	bayes_pls_golite062009
nucleic acid binding	0.699955808682622	bayes_pls_golite062009
carbon-sulfur lyase activity	0.698312655247044	bayes_pls_golite062009
transcription factor activity	0.597417107954964	bayes_pls_golite062009
5-aminolevulinate synthase activity	0.54326874956263	bayes_pls_golite062009
cystathionine gamma-lyase activity	0.505859361763689	bayes_pls_golite062009
transferase activity, transferring acyl groups	0.50269055590758	bayes_pls_golite062009
cystathionine beta-lyase activity	0.27933426619091	bayes_pls_golite062009
glycine hydroxymethyltransferase activity	0.271623203801431	bayes_pls_golite062009
O-phospho-L-serine:2-oxoglutarate aminotransferase activity	0.234399054481664	bayes_pls_golite062009
RNA binding	0.222777224430863	bayes_pls_golite062009
vitamin B6 binding	0.16957228630576	bayes_pls_golite062009
pyridoxal phosphate binding	0.16957228630576	bayes_pls_golite062009
methyltransferase activity	0.103986924428507	bayes_pls_golite062009
sulfurtransferase activity	0.0450490929307473	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	0.0254276656964899	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: