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View Structure Prediction Details

Protein: MET13
Organism: Saccharomyces cerevisiae
Length: 600 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MET13.

Description E-value Query
Range
Subject
Range
MTHR2_YEAST - Methylenetetrahydrofolate reductase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ME...
MET13 - Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetra...
gi|151943685 - gi|151943685|gb|EDN61995.1| methionine requiring protein [Saccharomyces cerevisiae YJM789]
0.0 [1..600] [1..600]
gi|22040813 - gi|22040813|gb|AAL91367.2|AF441241_1 chimera1 [synthetic construct]
0.0 [1..590] [1..588]
gi|42554286, gi|... - gi|46136113|ref|XP_389748.1| hypothetical protein FG09572.1 [Gibberella zeae PH-1], gi|42554286|gb|E...
0.0 [1..600] [1..584]
MTHR_ORYSA - Probable methylenetetrahydrofolate reductase (EC 1.5.1.20) - Oryza sativa (Rice)
gi|125546191 - gi|125546191|gb|EAY92330.1| hypothetical protein OsI_14054 [Oryza sativa Indica Group]
MTHR_ORYSJ - Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica GN=Os03g0815200 PE=2 SV...
0.0 [1..590] [1..586]
gi|67539426, gi|... - gi|67539426|ref|XP_663487.1| hypothetical protein AN5883.2 [Aspergillus nidulans FGSC A4], ref|XP_41...
gi|113911583 - gi|113911583|gb|ABI48273.1| methylenetetrahydrofolate reductase [Emericella nidulans]
0.0 [1..596] [1..580]
MTHR1_MAIZE - Methylenetetrahydrofolate reductase 1 OS=Zea mays PE=2 SV=1
0.0 [1..590] [1..586]
MTHR1_ARATH - Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana GN=MTHFR1 PE=1 SV=1
0.0 [1..590] [1..586]
gi|4753778 - gi|4753778|emb|CAB41971.1| methylenetetrahydrofolate reductase [Homo sapiens]
0.0 [2..596] [67..659]

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Predicted Domain #1
Region A:
Residues: [1-309]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKITEKLEQH RQTSGKPTYS FEYFVPKTTQ GVQNLYDRMD RMYEASLPQF IDITWNAGGG  60
   61 RLSHLSTDLV ATAQSVLGLE TCMHLTCTNM PISMIDDALE NAYHSGCQNI LALRGDPPRD 120
  121 AENWTPVEGG FQYAKDLIKY IKSKYGDHFA IGVAGYPECH PELPNKDVKL DLEYLKQKID 180
  181 AGGDFIITQM FYDVDNFINW CSQVRAAGMD VPIIPGIMPI TTYAAFLRRA QWGQISIPQH 240
  241 FSSRLDPIKD DDELVRDIGT NLIVEMCQKL LDSGYVSHLH IYTMNLEKAP LMILERLNIL 300
  301 PTESEFNAH

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 94.39794
Match: 1v93A_
Description: 5,10-Methylenetetrahydrofolate Reductase from Thermus thermophilus HB8
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
methylenetetrahydrofolate reductase (NADPH) activity 3.97406471031265 bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH group of donors 3.43788898294589 bayes_pls_golite062009
oxidoreductase activity 3.26500423104107 bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor 2.35093189018748 bayes_pls_golite062009
catalytic activity 2.15527229494088 bayes_pls_golite062009
1-pyrroline-5-carboxylate dehydrogenase activity 2.11575302268542 bayes_pls_golite062009
binding 1.55766253746824 bayes_pls_golite062009
transcription regulator activity 0.413628997019645 bayes_pls_golite062009
nucleic acid binding 0.385543917258092 bayes_pls_golite062009
protein binding 0.229798056306674 bayes_pls_golite062009
DNA binding 0.214617895050106 bayes_pls_golite062009

Predicted Domain #2
Region A:
Residues: [310-453]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PLAVLPWRKS LNPKRKNEEV RPIFWKRRPY SYVARTSQWA VDEFPNGRFG DSSSPAFGDL  60
   61 DLCGSDLIRQ SANKCLELWS TPTSINDVAF LVINYLNGNL KCLPWSDIPI NDEINPIKAH 120
  121 LIELNQHSII TINSQPQVNG IRSN

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

    MCM
Score
GO
Score
GO
Term
SCOP
Match
SCOP Description
View Download 0.547 0.824 methylenetetrahydrofolate reductase (NADPH) activity c.37.1 P-loop containing nucleoside triphosphate hydrolases

Predicted Domain #3
Region A:
Residues: [454-508]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DKIHGWGPKD GYVYQKQYLE FMLPKTKLPK LIDTLKNNEF LTYFAIDSQG DLLSN

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #4
Region A:
Residues: [509-600]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 HPDNSKSNAV TWGIFPGREI LQPTIVEKIS FLAWKEEFYH ILNEWKLNMN KYDKPHSAQF  60
   61 IQSLIDDYCL VNIVDNDYIS PDDQIHSILL SL

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.


YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle