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View Structure Prediction Details

Protein: PUF2
Organism: Saccharomyces cerevisiae
Length: 1075 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PUF2.

Description E-value Query
Range
Subject
Range
PUF2 - Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that ...
PUF2_YEAST - mRNA-binding protein PUF2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUF2 PE=1 SV=1
0.0 [1..1075] [1..1075]
PUM1_ARATH - Pumilio homolog 1 OS=Arabidopsis thaliana GN=APUM1 PE=1 SV=1
0.0 [24..867] [101..948]
gi|125572616, gi... - gi|34908236|ref|NP_915465.1| putative pumilio/Mpt5 family RNA-binding protein [Oryza sativa (japonic...
0.0 [64..867] [252..1031]

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Predicted Domain #1
Region A:
Residues: [1-279]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDNKRLYNGN LSNIPEVIDP GITIPIYEED IRNDTRMNTN ARSVRVSDKR GRSSSTSPQK  60
   61 IGSYRTRAGR FSDTLTNLLP SISAKLHHSK KSTPVVVVPP TSSTPDSLNS TTYAPRVSSD 120
  121 SFTVATPLSL QSTTTRTRTR NNTVSSQITA SSSLTTDVGN ATSANIWSAN AESNTSSSPL 180
  181 FDYPLATSYF EPLTRFKSTD NYTLPQTAQL NSFLEKNGNP NIWSSAGNSN TDHLNTPIVN 240
  241 RQRSQSQSTT NRVYTDAPYY QQPAQNYQVQ VPPRVPKST

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2
Region A:
Residues: [280-454]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SISPVILDDV DPASINWITA NQKVPLVNQI SALLPTNTIS ISNVFPLQPT QQHQQNAVNL  60
   61 TSTSLATLCS QYGKVLSART LRGLNMALVE FSTVESAICA LEALQGKELS KVGAPSTVSF 120
  121 ARVLPMYEQP LNVNGFNNTP KQPLLQEQLN HGVLNYQLQQ SLQQPELQQQ PTSFN

[Run NCBI BLAST on this sequence.]

Detection Method: Pfam
Confidence: 3.040959
Match: PF00076
Description: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #3
Region A:
Residues: [455-511]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QPNLTYCNPT QNLSHLQLSS NENEPYPFPL PPPSLSDSKK DILHTISSFK LEYDHLE

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #4
Region A:
Residues: [512-650]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LNHLLQNALK NKGVSDTNYF GPLPEHNSKV PKRKDTFDAP KLRELRKQFD SNSLSTIEME  60
   61 QLAIVMLDQL PELSSDYLGN TVIQKLFENS SNIIRDIMLR KCNKYLTSMG VHKNGTWVCQ 120
  121 KIIKMANTPR QINLVTSGV

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 200.0103
Match: 1ib2A_
Description: Pumilio 1
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
RNA binding 4.2310970544603 bayes_pls_golite062009
mRNA binding 3.1237691117539 bayes_pls_golite062009
nucleic acid binding 2.87272982027282 bayes_pls_golite062009
binding 2.24760917108348 bayes_pls_golite062009
1.12277203340218 bayes_pls_golite062009
single-stranded RNA binding 1.08300488237934 bayes_pls_golite062009
protein binding 0.361619805766015 bayes_pls_golite062009
hydrolase activity 0.181856784298799 bayes_pls_golite062009
poly-pyrimidine tract binding 0.137774615811754 bayes_pls_golite062009
transcription regulator activity 0.0600612010525731 bayes_pls_golite062009
DNA binding 0.0239003246213715 bayes_pls_golite062009
mRNA 3'-UTR binding 0.00441912341140061 bayes_pls_golite062009

Predicted Domain #5
Region A:
Residues: [651-751]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SDYCTPLFND QFGNYVIQGI LKFGFPWNSF IFESVLSHFW TIVQNRYGSR AVRACLEADS  60
   61 IITQCQLLTI TSLIIVLSPY LATDTNGTLL ITWLLDTCTL P

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 200.0103
Match: 1ib2A_
Description: Pumilio 1
Matching Structure (courtesy of the PDB):

Predicted Domain #6
Region A:
Residues: [752-899]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NKNLILCDKL VNKNLVKLCC HKLGSLTVLK ILNLRGGEEE ALSKNKIIHA IFDGPISSDS  60
   61 ILFQILDEGN YGPTFIYKVL TSRILDNSVR DEAITKIRQL ILNSNINLQS RQLLEEVGLS 120
  121 SAGISPKQSS KNHRKQHPQG FHSPGRAR

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 200.0103
Match: 1ib2A_
Description: Pumilio 1
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
RNA binding 2.11573661150436 bayes_pls_golite062009
binding 1.83406697369076 bayes_pls_golite062009
protein binding 1.04044934052933 bayes_pls_golite062009
nucleic acid binding 0.967597910409065 bayes_pls_golite062009
mRNA binding 0.73831124805124 bayes_pls_golite062009
hydrolase activity 0.0579816100130538 bayes_pls_golite062009
transcription regulator activity 0.022274123463527 bayes_pls_golite062009

Predicted Domain #7
Region A:
Residues: [900-1075]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GVSVSSVRSS NSRHNSVIQM NNAGPTPALN FNPAPMSEIN SYFNNQQVVY SGNQNQNQNG  60
   61 NSNGLDELNS QFDSFRIANG TNLSLPIVNL PNVSNNNNNY NNSGYSSQMN PLSRSVSHNN 120
  121 NNNTNNYNNN DNDNNNNNNN NNNNNNNNNN NNNNSNNSNN NNNNDTSLYR YRSYGY

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.


YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle