YRC Public Data Repository - Protein Structure Prediction

Protein:	PUP1
Organism:	Saccharomyces cerevisiae
Length:	261 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PUP1.

Description	E-value	Query Range	Subject Range
PUP1 - Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after ba... PSB2_YEAST - Proteasome subunit beta type-2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUP1 PE=1...	7.0E-73	[1..261]	[1..261]
gi\|5833465 - gi\|5833465\|gb\|AAD53521.1\|AF155581_1 proteasome subunit beta 7 [Danio rerio]	2.0E-72	[2..258]	[10..270]
gi\|12833090 - gi\|12833090\|dbj\|BAB22385.1\| unnamed protein product [Mus musculus]	7.0E-72	[1..258]	[11..272]
gi\|74136045, gi\|... - gi\|74136045\|ref\|NP_001027952.1\| proteasome Z subunit [Ciona intestinalis], gi\|2564233\|emb\|CAA05209.1...	2.0E-71	[2..258]	[13..270]
PSB7_RAT - Proteasome subunit beta type-7 OS=Rattus norvegicus GN=Psmb7 PE=1 SV=1	7.0E-71	[1..258]	[11..272]
gi\|62898273, gi\|... - gi\|62898273\|dbj\|BAD97076.1\| proteasome beta 7 subunit proprotein variant [Homo sapiens], gi\|62897575... gi\|114626634 - gi\|114626634\|ref\|XP_520247.2\| PREDICTED: proteasome beta 7 subunit isoform 5 [Pan troglodytes]	1.0E-70	[1..258]	[11..272]

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Predicted Domain #1

Region A:
Residues: [1-261]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAGLSFDNYQ RNNFLAENSH TQPKATSTGT TIVGVKFNNG VVIAADTRST QGPIVADKNC  60
   61 AKLHRISPKI WCAGAGTAAD TEAVTQLIGS NIELHSLYTS REPRVVSALQ MLKQHLFKYQ 120
  121 GHIGAYLIVA GVDPTGSHLF SIHAHGSTDV GYYLSLGSGS LAAMAVLESH WKQDLTKEEA 180
  181 IKLASDAIQA GIWNDLGSGS NVDVCVMEIG KDAEYLRNYL TPNVREEKQK SYKFPRGTTA 240
  241 VLKESIVNIC DIQEEQVDIT A

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1396.30103
Match:	1fntI_
Description:	CRYSTAL STRUCTURE OF THE 20S PROTEASOME FROM YEAST IN COMPLEX WITH THE PROTEASOME ACTIVATOR PA26 FROM TRYPANOSOME BRUCEI AT 3.2 ANGSTROMS RESOLUTION
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
endopeptidase activity	6.91642761177919	bayes_pls_golite062009
peptidase activity	6.84511694091156	bayes_pls_golite062009
peptidase activity, acting on L-amino acid peptides	6.5973571808298	bayes_pls_golite062009
hydrolase activity	4.47652590304822	bayes_pls_golite062009
threonine-type endopeptidase activity	2.98214579292788	bayes_pls_golite062009
threonine-type peptidase activity	2.98214579292788	bayes_pls_golite062009
catalytic activity	1.19663085909195	bayes_pls_golite062009
binding	0.982918122186083	bayes_pls_golite062009
nucleic acid binding	0.448212653390607	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: