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View Structure Prediction Details

Protein: YKL171W
Organism: Saccharomyces cerevisiae
Length: 928 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for YKL171W.

Description E-value Query
Range
Subject
Range
YKL171W - Putative protein kinase; implicated in proteasome function; epitope-tagged protein localizes to the ...
NNK1_YEAST - Nitrogen network kinase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NNK1 PE=1 SV=1
0.0 [1..928] [1..928]
CDPK2_ARATH - Calcium-dependent protein kinase 2 OS=Arabidopsis thaliana GN=CPK2 PE=1 SV=1
0.0 [282..897] [32..627]
gi|13561063 - gi|13561063|emb|CAA65500.1| protein kinase [Medicago sativa]
0.0 [342..901] [1..540]
gi|5162882, gi|5... - gi|5162882|dbj|BAA81751.1| calcium-dependent protein kinase [Marchantia polymorpha], gi|5162878|dbj|...
0.0 [372..897] [8..523]
gi|4336426 - gi|4336426|gb|AAD17800.1| Ca2+-dependent protein kinase [Mesembryanthemum crystallinum]
0.0 [352..902] [5..532]

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Predicted Domain #1
Region A:
Residues: [1-401]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFTSQRQLRQ NGSPMSSSRS SQHSSGTASP ISDSPASNRS YGRDLRGLMG IDIPANEPAF  60
   61 NRANSSDTIY FRPKKIYKME HEHPSRSTLV QLQTRSQPDD VASSQVNPEG GTDDLELGDP 120
  121 CGNQSLYTIG AEYVPDLDFT KLVNEWQKST EDLYEFRSSA TPQVQIKDSG KGNYELWSSP 180
  181 DAILTQNKLR RDSFSQENSD SLSPEDSILS RNLHSKVKPI PLPRNSQQIF TPLSNLEAER 240
  241 RSSYTTSSNN NSITQNNKFS FAKLKYSLPT QSSAVPASFD SNASSLNFLP TTTLSTLSEL 300
  301 QISPNDMMDL IQKLPRNFLN LPYTQRKKVI IEHAPSHDYK AMMSLVKKFM LTSSRSNFSL 360
  361 AGFANNASVS QATANDDNIN SRNTPNNSND TYVNTRPLQR S

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2
Region A:
Residues: [402-530]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 RHGSIASQFL SSFSPSMTSI AKMNSNPLSG SAGGSARPDD KGMEILGHRL GKIIGFGAWG  60
   61 IIRECFDIET GVGRVIKIVK FKGHQNIKKH VLREVAIWRT LKHNRILPLL DWKLDDNYAM 120
  121 YCLTERIND

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [739-781]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 YIGSLPYASP ELLRYSDARR SKSVEMHIYD SPDSSQSEIS AAS

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 167.54902
Match: 1a06__
Description: Calmodulin-dependent protein kinase
Matching Structure (courtesy of the PDB):

Predicted Domain #3
Region A:
Residues: [531-738]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GTLYDLVISW DEFKRSKIPF AERCRLTIFL SLQLLSALKY MHSKTIVHGD IKLENCLLQK  60
   61 EGKKSDWKVF LCDFGMSCHF DEKHVYRNDT FDENLSSGNS HRKRKSIEQT NLIKYPTTNF 120
  121 LPDDRTNDFD ASENLKYQFE NRKHQPFTPK GMVSSSSHSL KHLNQPSSSS SSNLFHKPAS 180
  181 QPQPQHRSPF HGRHKTTDFS NLEPEPSK

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 167.54902
Match: 1a06__
Description: Calmodulin-dependent protein kinase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
protein kinase activity 2.5838644943424 bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor 2.45273401254881 bayes_pls_golite062009
protein serine/threonine kinase activity 2.32609718275273 bayes_pls_golite062009
kinase activity 2.25271639892273 bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups 1.87817787385115 bayes_pls_golite062009
binding 1.41489581155645 bayes_pls_golite062009
catalytic activity 1.21655087018101 bayes_pls_golite062009
transferase activity 1.02325581375333 bayes_pls_golite062009
protein binding 0.481719236497011 bayes_pls_golite062009
ATP binding 0.43842367748426 bayes_pls_golite062009
adenyl ribonucleotide binding 0.395712570017132 bayes_pls_golite062009
adenyl nucleotide binding 0.381353295672519 bayes_pls_golite062009
purine ribonucleotide binding 0.222536651626411 bayes_pls_golite062009
ribonucleotide binding 0.222477512452933 bayes_pls_golite062009
purine nucleotide binding 0.213888799799513 bayes_pls_golite062009
nucleotide binding 0.208281379222054 bayes_pls_golite062009
peptidase activity 0.00308643640382985 bayes_pls_golite062009

Predicted Domain #4
Region A:
Residues: [782-928]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SSSSNLSSLS SSTKASAVTN SGVTTSSPSG SSTDFPCIVS PLGPASDIWA LGVMLYTMLV  60
   61 GKLPFNHEFE PRLRSLIKVG EFDRFSLAQV CKFDRKKNEG TIGQGLYDTV IGCLTIDLDK 120
  121 RWKLKRIEEV LQNEMNLSEA IHDNNGS

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #5
Region A:
Residues: [780-928]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 ASSSSSNLSS LSSSTKASAV TNSGVTTSSP SGSSTDFPCI VSPLGPASDI WALGVMLYTM  60
   61 LVGKLPFNHE FEPRLRSLIK VGEFDRFSLA QVCKFDRKKN EGTIGQGLYD TVIGCLTIDL 120
  121 DKRWKLKRIE EVLQNEMNLS EAIHDNNGS

[Run NCBI BLAST on this sequence.]

Detection Method: FFAS03
Confidence: 7.41
Match: 2eueA
Description: Crystal structure of the protein kinase domain of yeast AMP-activated protein kinase Snf1
Matching Structure (courtesy of the PDB):

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle