YRC Public Data Repository - Protein Structure Prediction

Protein:	THI3
Organism:	Saccharomyces cerevisiae
Length:	609 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for THI3.

Description	E-value	Query Range	Subject Range
gi\|17232105, gi\|... - gi\|17232105\|ref\|NP_488653.1\| acetolactate synthase 3 catalytic subunit [Nostoc sp. PCC 7120], gi\|171... gi\|25291313 - pir\|\|AE2382 acetohydroxy acid synthase [imported] - Nostoc sp. (strain PCC 7120)	0.0	[12..579]	[30..596]
gi\|7436698 - pir\|\|C70341 acetolactate synthase (EC 4.1.3.18) large chain - Aquifex aeolicus gi\|15605942, gi\|... - gi\|2983101\|gb\|AAC06706.1\| acetolactate synthase large subunit [Aquifex aeolicus VF5], gi\|15605942\|re...	0.0	[16..592]	[5..578]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[5..587]	[2..586]
gi\|152905 - gi\|152905\|gb\|AAA26594.1\| acetohydroxy acid synthase (AHAS) gi\|152905 - gi\|152905\|gb\|AAA26594.1\| acetohydroxy acid synthase (AHAS) [Arthrospira platensis]	0.0	[5..587]	[2..586]
gi\|447739 - gi\|447739\|prf\|\|1915304A nylB upstream ORF	0.0	[5..577]	[2..573]
Q66EL8\|Q66EL8_YERPS - Acetolactate synthase III, valine sensitive, large subunit FAD and thiamine PPi binding - Yersinia p... gi\|77631655, gi\|... - gi\|77631655\|ref\|ZP_00794241.1\| COG0028: Thiamine pyrophosphate-requiring enzymes [acetolactate synth... gi\|161511301 - gi\|161511301\|ref\|NP_994917.2\| acetolactate synthase 3 catalytic subunit [Yersinia pestis biovar Micr... gi\|161484748 - gi\|161484748\|ref\|NP_670937.2\| acetolactate synthase 3 catalytic subunit [Yersinia pestis KIM] gi\|186894031, gi... - gi\|186894031\|ref\|YP_001871143.1\| acetolactate synthase 3 catalytic subunit [Yersinia pseudotuberculo... gi\|169752280, gi... - gi\|170025746\|ref\|YP_001722251.1\| acetolactate synthase 3 catalytic subunit [Yersinia pseudotuberculo... gi\|229837069, gi... - gi\|229837069\|ref\|ZP_04457234.1\| acetolactate synthase isozyme III large subunit [Yersinia pestis Pes... gi\|150260425, gi... - gi\|150260425\|ref\|ZP_01917153.1\| acetolactate synthase isozyme III large subunit [Yersinia pestis CA8... gi\|229696794, gi... - gi\|229840428\|ref\|ZP_04460587.1\| acetolactate synthase isozyme III large subunit [Yersinia pestis bio... gi\|229843030, gi... - gi\|229843030\|ref\|ZP_04463180.1\| acetolactate synthase isozyme III large subunit [Yersinia pestis bio... gi\|89104759 - gi\|89104759\|ref\|ZP_01177293.1\| COG0028: Thiamine pyrophosphate-requiring enzymes [acetolactate synth... gi\|108809551, gi... - gi\|108809551\|ref\|YP_653467.1\| acetolactate synthase 3 catalytic subunit [Yersinia pestis Antiqua], g... gi\|77633509 - gi\|77633509\|ref\|ZP_00795637.1\| COG0028: Thiamine pyrophosphate-requiring enzymes [acetolactate synth... gi\|229682102, gi... - gi\|229900763\|ref\|ZP_04515887.1\| acetolactate synthase isozyme III large subunit [Yersinia pestis Nep... gi\|25291303 - pir\|\|AI0066 acetolactate synthase (EC 4.1.3.18) isozyme III large chain [imported] - Yersinia pestis... gi\|218927736, gi... - gi\|218927736\|ref\|YP_002345611.1\| acetolactate synthase 3 catalytic subunit [Yersinia pestis CO92], g... gi\|51588307, gi\|... - gi\|51595025\|ref\|YP_069216.1\| acetolactate synthase 3 catalytic subunit [Yersinia pseudotuberculosis ...	0.0	[13..577]	[2..567]
gi\|7436699 - pir\|\|S75115 acetohydroxy acid synthase - Synechocystis sp. (strain PCC 6803) gi\|1653060, gi\|1... - gi\|1653060\|dbj\|BAA17977.1\| acetohydroxy acid synthase [Synechocystis sp. PCC 6803], gi\|16330569\|ref\|...	0.0	[8..577]	[14..582]
gi\|6478282 - gi\|6478282\|gb\|AAF13791.1\|AF129502_2 acetohydroxy acid synthase large subunit [Buchnera aphidicola] ILVI_BUCSC - Acetolactate synthase large subunit OS=Buchnera aphidicola subsp. Schlechtendalia chinensis GN=ilvI ...	0.0	[13..567]	[2..556]
gi\|13958149 - gi\|13958149\|gb\|AAK50820.1\|AF363369_1 acetolactate synthase [Amaranthus retroflexus]	0.0	[3..580]	[80..662]

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Predicted Domain #1

Region A:
Residues: [1-199]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNSSYTQRYA LPKCIAISDY LFHRLNQLNI HTIFGLSGEF SMPLLDKLYN IPNLRWAGNS  60
   61 NELNAAYAAD GYSRLKGLGC LITTFGVGEL SAINGVAGSY AEHVGILHIV GMPPTSAQTK 120
  121 QLLLHHTLGN GDFTVFHRIA SDVACYTTLI IDSELCADEV DKCIKKAWIE QRPVYMGMPV 180
  181 NQVNLPIESA RLNTPLDLQ

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1921.0
Match:	1qpbA_
Description:	Pyruvate decarboxylase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
acetolactate synthase activity	12.903449876837	bayes_pls_golite062009
transferase activity, transferring aldehyde or ketonic groups	12.3239025230705	bayes_pls_golite062009
pyruvate dehydrogenase activity	5.28857071459273	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor	5.21233670052586	bayes_pls_golite062009
pyruvate dehydrogenase (acetyl-transferring) activity	5.08663245333808	bayes_pls_golite062009
NAD or NADH binding	3.93430700665136	bayes_pls_golite062009
transcription regulator activity	2.9155913558118	bayes_pls_golite062009
DNA binding	2.43141001866996	bayes_pls_golite062009
nucleic acid binding	2.41433477472968	bayes_pls_golite062009
catalytic activity	2.25537597692103	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors	2.15444163802756	bayes_pls_golite062009
transketolase activity	2.13705987575498	bayes_pls_golite062009
NAD-dependent protein deacetylase activity	2.09493047697397	bayes_pls_golite062009
NAD-dependent histone deacetylase activity	2.09493047697397	bayes_pls_golite062009
histone deacetylase activity	2.07818242335598	bayes_pls_golite062009
protein deacetylase activity	2.07076853700185	bayes_pls_golite062009
deoxyhypusine synthase activity	2.04735838056767	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	1.98546381612935	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	1.82969293063325	bayes_pls_golite062009
hydrolase activity	1.43067365161346	bayes_pls_golite062009
transcription factor activity	1.30143289828088	bayes_pls_golite062009
deacetylase activity	1.21596867135219	bayes_pls_golite062009
binding	1.17940168894417	bayes_pls_golite062009
transcription factor binding	1.14766678956419	bayes_pls_golite062009
phenylpyruvate decarboxylase activity	1.11553401986553	bayes_pls_golite062009
3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity	1.07090849835369	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	1.0611726850452	bayes_pls_golite062009
protein binding	0.992849906276347	bayes_pls_golite062009
cation transmembrane transporter activity	0.78591260444574	bayes_pls_golite062009
alpha-ketoacid dehydrogenase activity	0.750929351082237	bayes_pls_golite062009
transcription repressor activity	0.614654207326026	bayes_pls_golite062009
transcription cofactor activity	0.54005617175343	bayes_pls_golite062009
ion transmembrane transporter activity	0.51426028502998	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH	0.492590326775569	bayes_pls_golite062009
transferase activity	0.413024577830522	bayes_pls_golite062009
transcription corepressor activity	0.40721151987565	bayes_pls_golite062009
pyruvate decarboxylase activity	0.198062360371269	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	0.114062221123972	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [200-364]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LHKNDPDVEK EVISRILSFI YKSQNPAIIV DACTSRQNLI EETKELCNRL KFPVFVTPMG  60
   61 KGTVNETDPQ FGGVFTGSIS APEVREVVDF ADFIIVIGCM LSEFSTSTFH FQYKTKNCAL 120
  121 LYSTSVKLKN ATYPDLSIKL LLQKILANLD ESKLSYQPSE QPSMM

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1921.0
Match:	1qpbA_
Description:	Pyruvate decarboxylase
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [365-609]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 VPRPYPAGNV LLRQEWVWNE ISHWFQPGDI IITETGASAF GVNQTRFPVN TLGISQALWG  60
   61 SVGYTMGACL GAEFAVQEIN KDKFPATKHR VILFMGDGAF QLTVQELSTI VKWGLTPYIF 120
  121 VMNNQGYSVD RFLHHRSDAS YYDIQPWNYL GLLRVFGCTN YETKKIITVG EFRSMISDPN 180
  181 FATNDKIRMI EIMLPPRDVP QALLDRWVVE KEQSKQVQEE NENSSAVNTP TPEFQPLLKK 240
  241 NQVGY

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1921.0
Match:	1qpbA_
Description:	Pyruvate decarboxylase
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: