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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #33 Asynchronous SPB prep

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 12 12 91.3% 0.275 7729
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 11 12 70.7% 0.129 16135
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 15 17 44.1% 0.0741 42271
[View Peptides]   SPA1_STAAU (P02976) Immunoglobulin G binding protein A precursor (IgG binding protein A) (Staphylococcal protei... 21 25 36.5% 0.0755 57320
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 5 5 31.7% 0.0492 18751
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 9 14 25.3% 0.0876 29280
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 8 8 23.9% 0.0218 67400
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 19 20 22.2% 0.0335 111782
[View Peptides]   Reverse_YPR020W ATP20 SGDID:S000006224, Chr XVI from 599867-600214, Verified ORF, "Subunit g of the mitochondrial F1... 2 2 20.9% 0.0273 12921
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 31 41 18.2% 0.0387 195140
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 14 16 14.7% 0.0298 94104
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 4 4 14.0% 0.0164 45384
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 3 3 9.8% 0.00827 66087
[View Peptides]   CDC14 Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... 3 3 9.8% 0.00862 61907
[View Peptides]   RPL5 Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... 1 1 9.4% 0.00533 33715
[View Peptides]   RPS0B Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Ap; required for mat... 1 1 9.1% 0.00628 27962
[View Peptides]   gi|2119236, gi|4... sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... 2 2 8.7% 0.00509 61987
[View Peptides]   RPS3 Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease ... 1 1 7.1% 0.0066 26503
[View Peptides]   SSB2 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 2 2 6.9% 0.00516 66595
[View Peptides]   SSB1 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 2 2 6.9% 0.00516 66602
[View Peptides]   gi|67568 pir||ELPG pancreatic elastase (EC 3.4.21.36) I precursor - pig 1 1 6.8% 0.00595 28821
[View Peptides]   SSA2 ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... 2 2 6.6% 0.00495 69470
[View Peptides]   SSA1 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... 2 2 6.5% 0.00493 69768
[View Peptides]   RRB1 Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with ... 1 1 6.3% 0.0031 57261
[View Peptides]   MRPL3 Mitochondrial ribosomal protein of the large subunit 1 1 6.2% 0.00406 44000
[View Peptides]   HSP42 Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress... 1 1 6.1% 0.00422 42817
[View Peptides]   NPL3 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... 1 1 5.3% 0.00382 45407
[View Peptides]   MRM1 Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondria... 1 1 4.9% 0.00384 46387
[View Peptides]   TUB4 Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... 1 1 4.7% 0.00335 52628
[View Peptides]   IDP3 Peroxisomal NADP-dependent isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-keto... 1 1 4.5% 0.00377 47856
[View Peptides]   DEF1 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... 2 2 4.2% 0.00429 83973
[View Peptides]   ALD4 Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde ... 1 1 3.9% 0.00305 56724
[View Peptides]   Reverse_YIL022W TIM44 SGDID:S000001284, Chr IX from 311163-312458, Verified ORF, "Peripheral mitochondrial membrane ... 1 1 3.7% 0.00366 48854
[View Peptides]   gi|2144511 pir||HYBST thermolysin (EC 3.4.24.27) precursor [validated] - Bacillus "thermoproteolyticus 1 1 3.5% 0.00289 60122
[View Peptides]   SPN1 Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II, probable transcri... 1 1 3.4% 0.00386 46083
[View Peptides]   YJL045W Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxi... 1 1 3.2% 0.0025 69383
[View Peptides]   CDC53 Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box pr... 1 1 3.1% 0.00194 93944
[View Peptides]   RAP1 DNA-binding protein involved in either activation or repression of transcription, depending on bindi... 1 1 3.1% 0.00191 92413
[View Peptides]   ENA5 Protein with similarity to P-type ATPase sodium pumps, member of the Na+ efflux ATPase family 1 1 2.9% 0.00145 120296
[View Peptides]   Reverse_YPL256C CLN2 SGDID:S000006177, Chr XVI from 66614-64977, reverse complement, Verified ORF, "G1 cyclin involv... 1 1 2.9% 0.0029 61697
[View Peptides]   ENA1 P-type ATPase sodium pump, involved in Na+ and Li+ efflux to allow salt tolerance 1 1 2.9% 0.00145 120357
[View Peptides]   ENA2 P-type ATPase sodium pump, involved in Na+ efflux to allow salt tolerance; likely not involved in Li... 1 1 2.9% 0.00145 120317
[View Peptides]   MPS2 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... 1 1 2.8% 0.00409 44585
[View Peptides]   Reverse_YJR091C JSN1 SGDID:S000003851, Chr X from 598250-594975, reverse complement, Verified ORF, "Member of the Pu... 1 1 2.7% 0.00145 120374
[View Peptides]   Reverse_YDR108W GSG1 SGDID:S000002515, Chr IV from 671264-673360, Verified ORF, "Subunit of TRAPP (transport protein... 1 1 2.7% 0.00226 80495
[View Peptides]   Reverse_YOR154W YOR154W SGDID:S000005680, Chr XV from 624729-626492, Uncharacterized ORF, "Hypothetical protein" 1 1 2.7% 0.00269 67452
[View Peptides]   MNN4 Putative positive regulator of mannosylphosphate transferase (Mnn6p), involved in mannosylphosphoryl... 1 1 1.8% 0.00134 139380
[View Peptides]   YIL169C Putative protein of unknown function; serine/threonine rich and highly similar to YOL155C, a putativ... 1 1 1.8% 0.00159 99736
[View Peptides]   Reverse_Contamin... owl|P44969| IMMUNOGLOBULIN A1 PROTEASE PRECURSOR (EC 3.4.21.72) (IGA1 PROTEASE)...., owl|P44969| [co... 1 1 1.4% 9.34E-4 185538
[View Peptides]   EPL1 Component of NuA4, which is an essential histone H4/H2A acetyltransferase complex; homologous to Dro... 1 1 1.2% 0.0019 96738
[View Peptides]   gi|35830 gi|35830|emb|CAA40296.1| ubiquitin activating enzyme E1 [Homo sapiens] 1 1 0.7% 0.0015 117790

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