Bait Protein: | VAM3 |
Organism: | Saccharomyces cerevisiae |
Publication: | Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press] |
Comments: | sample: hx1 |
Raw Data: |
Download DTASelect Filter text Download DTASelect HTML file |
UNFILTERED Run Results:
[filter] [View our MS interpretation guidelines.] |
|||||||
Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
SEC17 | Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' ste... | 13 | 37 | 44.5% | 0.0858 | 32803 |
[ |
KRT10 | keratin 10 | 23 | 78 | 39.0% | 0.089 | 59519 |
[ |
Contaminant_KERA... | 34 | 93 | 35.9% | 0.0979 | 65494 | |
[ |
Contaminant_KERA... | 19 | 28 | 35.7% | 0.0402 | 51531 | |
[ |
contaminant_KERA... | 23 | 52 | 33.3% | 0.0626 | 59822 | |
[ |
gi|2119236, gi|4... | sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... | 26 | 68 | 32.5% | 0.074 | 61987 |
[ |
contaminant_KERA... | 18 | 24 | 32.4% | 0.0346 | 50499 | |
[ |
NYV1 | v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... | 5 | 6 | 30.0% | 0.0161 | 28964 |
[ |
KRT2 | keratin 2 | 22 | 54 | 29.3% | 0.0567 | 65865 |
[ |
VAM7 | Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... | 8 | 18 | 27.5% | 0.0386 | 36711 |
[ |
VTI1 | Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... | 5 | 13 | 26.7% | 0.0406 | 24668 |
[ |
RPP2A | Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... | 2 | 2 | 24.5% | 0.0128 | 10746 |
[ |
RPP2B | Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... | 2 | 2 | 23.6% | 0.0123 | 11050 |
[ |
KRT5 | keratin 5 | 16 | 37 | 23.4% | 0.0425 | 62461 |
[ |
RPL36B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl36Ap and has similari... | 2 | 2 | 22.0% | 0.0135 | 11135 |
[ |
RPL36A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... | 2 | 2 | 22.0% | 0.0135 | 11124 |
[ |
VAM3 | Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... | 5 | 15 | 18.4% | 0.0359 | 32498 |
[ |
LYSC_LYSEN | Lysyl endopeptidase OS=Lysobacter enzymogenes PE=1 SV=1 | 7 | 8 | 18.2% | 0.0201 | 27961 |
[ |
KRT17 | keratin 17 | 8 | 10 | 17.6% | 0.0157 | 47974 |
[ |
PHO8 | Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... | 6 | 6 | 15.9% | 0.00718 | 63004 |
[ |
contaminant_KERA... | isb|A33652|A33652|probable keratin 16, type I, pseudogene - human | 10 | 19 | 14.2% | 0.0272 | 50915 |
[ |
YKT6 | Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and wit... | 2 | 2 | 13.0% | 0.00677 | 22707 |
[ |
RPL4A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... | 3 | 4 | 12.4% | 0.00748 | 39092 |
[ |
RPL4B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarit... | 3 | 4 | 12.4% | 0.00748 | 39062 |
[ |
SSA2 | ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... | 5 | 5 | 12.1% | 0.0053 | 69470 |
[ |
ROT1 | Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synt... | 2 | 2 | 12.1% | 0.00529 | 28908 |
[ |
KRT19 | keratin 19 | 7 | 9 | 11.8% | 0.0152 | 44106 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 4 | 4 | 11.6% | 0.00542 | 54545 |
[ |
RPS6A, RPS6B | Protein component of the small (40S) ribosomal subunit; identical to Rps6Bp and has similarity to ra... | 2 | 2 | 11.4% | 0.00574 | 26996 |
[ |
KRT3 | keratin 3 | 8 | 38 | 9.9% | 0.0409 | 64511 |
[ |
RPL8B | Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has simila... | 2 | 3 | 9.0% | 0.00793 | 28112 |
[ |
RPL8A | Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Bp and has simila... | 2 | 3 | 9.0% | 0.00793 | 28125 |
[ |
SHM2 | Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrof... | 3 | 3 | 8.7% | 0.00433 | 52219 |
[ |
PHO84 | High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... | 3 | 3 | 8.0% | 0.00346 | 64382 |
[ |
VPH1 | Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... | 4 | 4 | 7.3% | 0.00322 | 95529 |
[ |
gi|34033, gi|71526 | pir||KRHU3 keratin 13, type I, cytoskeletal, long splice form - human, gi|34033|emb|CAA32786.1| ker... | 4 | 9 | 7.0% | 0.0133 | 49644 |
[ |
PDA1 | E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarbo... | 2 | 2 | 6.4% | 0.00322 | 46343 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 2 | 2 | 4.5% | 0.00199 | 74468 |
[ |
VTC4 | Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) comp... | 3 | 3 | 4.2% | 0.00282 | 83155 |
[ |
RGA1 | GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of se... | 2 | 2 | 4.0% | 0.00134 | 112831 |
[ |
Contaminant_KERA... | 2 | 9 | 3.9% | 0.0114 | 57265 | |
[ |
VAM6 | Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... | 2 | 2 | 3.3% | 0.00129 | 122881 |
[ |
YCF1 | Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... | 3 | 3 | 3.0% | 0.00134 | 171120 |