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Protein Overview: BIG3_ARATH

General Information:
Name(s) found: BIG3_ARATH [Swiss-Prot]
Description(s) found:
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  • gi|8570447|gb|AAF76474.1|AC020622_8 Contains similarity to a guanine nucleotide exchange factor from Homo sapiens gb|AF111162 and contains a Sec7 PF|01369 domain. [Arabidopsis thaliana] [NCBI NR]
  • pir||E86151 hypothetical protein F22M8.9 - Arabidopsis thaliana [NCBI NR]
  • gi|15217579|ref|NP_171698.1| EDA10 (embryo sac development arrest 10); ARF guanyl-nucleotide exchange factor/ binding / guanyl-nucleotide exchange factor [Arabidopsis thaliana] [NCBI NR]
  • Brefeldin A-inhibited guanine nucleotide-exchange protein 3 OS=Arabidopsis thaliana GN=BIG3 PE=1 SV=1 [Swiss-Prot]
  • (Q9LPC5) F22M8.9 protein [athaliana3702.FASTAC.txt]
  • hypothetical protein F22M8.9 - Arabidopsis thalianagi|8570447|gb|AAF76474.1| Contains similarity to a guanine nucleotide exchange factor from Homo sapiens gb|AF111162 and contains a Sec7 PF|01369 domain. [Arabidopsis thaliana]gi|15217579|ref|NP_171698.1 [nrdb_11152004_Chlamydomonas]
  • Tax_Id=3702 Guanine nucleotide exchange family protein [EBI-IPI_Arabidopsis_3.11_12-06-2005_reversed.fasta]
  • | Symbols: EDA10 | EDA10 (embryo sac development arrest 10); guanyl-nucleotide exchange factor | chr1:330830-337582 REVERSE [TAIR_Arabidopsis_na_04-12-2008_con_reversed.fasta]
  • Contains similarity to a guanine nucleotide exchange factor from Homo sapiens gb|AF111162 and contains a Sec7 PF|01369 domain. [Arabidopsis thaliana] [NCBI-RefSeq_human_na_10-15-2008_con_reversed.fasta]
  • guanyl-nucleotide exchange factor [Arabidopsis thaliana] [nr-271106-contam.fasta]
  • | Symbols: EDA10 | SEC7-like guanine nucleotide exchange family protein | chr1:330830-337582 REVERSE LENGTH=1750 [TAIR_A_Thaliana_10_12-14-2010_reversed.fasta]
    • Organism: Arabidopsis thaliana
    Length: 1750 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDA]
    cytosol [IDA]
    Biological Process: megagametogenesis [IMP]
    Molecular Function: ARF guanyl-nucleotide exchange factor activity [IEA]
    guanyl-nucleotide exchange factor activity [ISS]
    binding [IEA]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MASTEVDSRL GRVVIPALDK VIKNASWRKH SKLAHECKSV IERLRSPENS SPVADSESGS  60
   61 SIPGPLHDGG AAEYSLAESE IILSPLINAS STGVLKIVDP AVDCIQKLIA HGYVRGEADP 120
  121 TGGPEALLLS KLIETICKCH ELDDEGLELL VLKTLLTAVT SISLRIHGDS LLQIVRTCYG 180
  181 IYLGSRNVVN QATAKASLVQ MSVIVFRRME ADSSTVPIQP IVVAELMEPM DKSESDPSTT 240
  241 QSVQGFITKI MQDIDGVFNS ANAKGTFGGH DGAFETSLPG TANPTDLLDS TDKDMLDAKY 300
  301 WEISMYKSAL EGRKGELADG EVEKDDDSEV QIGNKLRRDA FLVFRALCKL SMKTPPKEDP 360
  361 ELMRGKIVAL ELLKILLENA GAVFRTSDRF LGAIKQYLCL SLLKNSASNL MIIFQLSCSI 420
  421 LLSLVSRFRA GLKAEIGVFF PMIVLRVLEN VAQPDFQQKM IVLRFLDKLC VDSQILVDIF 480
  481 INYDCDVNSS NIFERMVNGL LKTAQGVPPG TVTTLLPPQE AAMKLEAMKC LVAVLRSMGD 540
  541 WVNKQLRLPD PYSAKMLEIV DRNLEEGSHP VENGKGDGGH GGFERSDSQS ELSSGNSDAL 600
  601 AIEQRRAYKL ELQEGISIFN QKPKKGIEFL IKANKVGDSP EEIAAFLKDA SGLNKTLIGD 660
  661 YLGEREDLSL KVMHAYVDSF EFQGMEFDEA IRAFLRGFRL PGEAQKIDRI MEKFAERFCK 720
  721 CNPKDFSSAD TAYVLAYSVI LLNTDAHNPM VKSKMTADGF IRNNRGIDDG KDLPEEYLRA 780
  781 LYERISRNEI KMKDDGLGPQ QKQPTNSSRL LGLDTILNIV VPRRGDDMNM ETSDDLIRHM 840
  841 QERFKEKARK SESVYYAASD VIILRFMVEV CWAPMLAAFS VPLDQSDDAV ITTLCLEGFH 900
  901 HAIHVTSVMS LKTHRDAFVT SLAKFTSLHS PADIKQKNIE AIKAIVKLAE EEGNYLQDAW 960
  961 EHILTCVSRF EHLHLLGEGA PPDATFFAFP QTESGNSPLA KPNSVPAIKE RAPGKLQYAA1020
 1021 SAMIRGSYDG SGVAGKASNT VTSEQMNNLI SNLNLLEQVG DMSRIFTRSQ RLNSEAIIDF1080
 1081 VKALCKVSMD ELRSPSDPRV FSLTKIVEIA HYNMNRIRLV WSSIWHVLSD FFVTIGCSDN1140
 1141 LSIAIFAMDS LRQLSMKFLE REELANYNFQ NEFMKPFVVV MRKSGAVEIR ELIIRCVSQM1200
 1201 VLSRVDNVKS GWKSMFMIFT TAAHDAHKNI VFLSFEMVEK IIRDYFPHIT ETETTTFTDC1260
 1261 VNCLVAFTNC KFEKDISLQA IAFLQYCARK LAEGYVGSSL RRNPPLSPQG GKIGKQDSGK1320
 1321 FLESDEHLYS WFPLLAGLSE LSFDPRAEIR KVALKVLFDT LRNHGDHFSL ALWERVFESV1380
 1381 LFRIFDYVRQ DVDPSEDDST DQRGYNGEVD QESWLYETCS LALQLVVDLF VNFYKTVNPL1440
 1441 LKKVLMLFVS LIKRPHQSLA GAGIAALVRL MRDVGHQFSN EQWLEVVSCI KEAADATSPD1500
 1501 FSYVTSEDLM EDVSNEDETN DNSNDALRRR NRQLHAVVTD AKSKASIQIF VIQAVTDIYD1560
 1561 MYRMSLTANH MLMLFDAMHG IGSNAHKINA DLLLRSKLQE LGSSLESQEA PLLRLENESF1620
 1621 QTCMTFLDNL ISDQPVGYNE AEIESHLISL CREVLEFYIN ISCSKEQSSR WAVPSGSGKK1680
 1681 KELTARAPLV VAAIQTLGNM GESLFKKNLP ELFPLIATLI SCEHGSGEVQ VALSDMLQTS1740
 1741 MGPVLLRSCC

    Protein Complex Data

    No complex found for this protein.

    Mass Spectrometry Data

    No mass spectrometry results found for this protein.

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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    No microscopy data found in the PDR for this protein.

    Protein Structure Data


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    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..222] MSA 3.128988 View MSA. No confident structure predictions are available.
    2 View Details [223..380] deduced N/A No confident structure predictions are available.
    3 View Details [381..548] MSA 1.225997 View MSA. No confident structure predictions are available.
    4 View Details [549..996] PSI-BLAST 79.39794 No description for 2r0dA was found.
    5 View Details [997..1750] PSI-BLAST 50.69897 Kap95p:RanGTP complex


    Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.89

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle