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Protein Overview: EG14217

General Information:
Name(s) found: hyfI / EG14217 [EcoGene]
Description(s) found:
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  • hydrogenase 4, Fe-S subunit / Hydrogenase 4 component I; membrane protein of Hyf electron transfer complex of FHL system [EcoGene]
  • hydrogenase 4, Fe-S subunit [Escherichia coli K12] [nr-271106-contam.fasta]
  • [wormpep210-phg-orf-LaDeana-ecoli-contam.fasta.nr]
  • hydrogenase 4, Fe-S subunit [Escherichia coli str. K-12 substr. MG1655] [Ecoli-contam-Dam1-Mind-COMA-MBP-110.fasta]
    • Organism: Escherichia coli str. K-12 substr. MG1655
    Length: 252 amino acids

    Gene Ontology:
    Cellular Component: NONE FOUND
    Biological Process: oxidation reduction [IEA]
    Molecular Function: quinone binding [IEA]
    metal ion binding [IEA]
    oxidoreductase activity [IEA]
    iron ion binding [IEA]
    NADH dehydrogenase (ubiquinone) activity [IEA]
    4 iron, 4 sulfur cluster binding [IEA]
    oxidoreductase activity, acting on NADH or NADPH [IEA]
    iron-sulfur cluster binding [IEA]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSPVLTQHVS QPITLDEQTQ KMKRHLLQDI RRSAYVYRVD CGGCNACEIE IFAAITPVFD  60
   61 AERFGIKVVS SPRHADILLF TGAVTRAMRM PALRAYESAP DHKICVSYGA CGVGGGIFHD 120
  121 LYSVWGGSDT IVPIDVWIPG CPPTPAATIH GFAVALGLLQ QKIHAVDYRD PTGVTMQPLW 180
  181 PQIPPSQRIA IEREARRLAG YRQGREICDR LLRHLSDDPT GNRVNTWLRD ADDPRLNSIV 240
  241 QQLFRVLRGL HD

    Protein Complex Data

    No complex found for this protein.

    Mass Spectrometry Data

    No mass spectrometry results found for this protein.

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..172] PSI-BLAST 47.30103 Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus
    2 View Details [173..252] deduced N/A No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • oxidoreductase activity, acting on hydrogen as donor
    		•  2.20752608202439 bayes_pls_golite062009
  • ferredoxin hydrogenase activity
    		•  1.32333392056778 bayes_pls_golite062009
  • oxidoreductase activity, acting on hydrogen as donor, iron-sulfur protein as acceptor
    		•  1.32333392056778 bayes_pls_golite062009
  • NADH dehydrogenase activity
    		•  0.84569674518508 bayes_pls_golite062009
  • oxidoreductase activity, acting on NADH or NADPH
    		•  0.554599825830849 bayes_pls_golite062009
  • catalytic activity
    		•  0.513070432311783 bayes_pls_golite062009
  • NADH dehydrogenase (quinone) activity
    		•  0.463241887648122 bayes_pls_golite062009
  • oxidoreductase activity, acting on iron-sulfur proteins as donors
    		•  0.39909556472213 bayes_pls_golite062009
  • oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor
    		•  0.314635495446813 bayes_pls_golite062009
  • oxidoreductase activity
    		•  0.0918357876652069 bayes_pls_golite062009
    2 No functions predicted.


    Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.70

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle