YRC Public Data Repository - Protein Structure Prediction

Protein:	GLGX_ECOLI
Organism:	Escherichia coli
Length:	657 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for GLGX_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|68198015, gi\|... - gi\|68234365\|ref\|ZP_00573449.1\| Glycoside hydrolase, family 13, N-terminal:Alpha amylase, catalytic d...	557.0	[0..1]	[657..803]
GLGX_ECO55 - Glycogen debranching enzyme OS=Escherichia coli (strain 55989 / EAEC) GN=glgX PE=3 SV=1 gi\|191167324, gi... - gi\|75229558\|ref\|ZP_00716101.1\| COG1523: Type II secretory pathway, pullulanase PulA and related glyc...	556.0	[0..1]	[657..1]
GLGX_ECO24 - Glycogen debranching enzyme OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=glgX PE=3 SV=1	556.0	[0..1]	[657..1]
gi\|193068774, gi... - gi\|75237034\|ref\|ZP_00721090.1\| COG1523: Type II secretory pathway, pullulanase PulA and related glyc...	555.0	[0..1]	[657..1]
gi\|194431339, gi... - gi\|83571645\|ref\|ZP_00923085.1\| COG1523: Type II secretory pathway, pullulanase PulA and related glyc...	555.0	[0..1]	[657..1]
gi\|58582972, gi\|... - gi\|58582972\|ref\|YP_201988.1\| glycogen debranching enzyme [Xanthomonas oryzae pv. oryzae KACC10331], ...	555.0	[0..2]	[656..28]
GLGX_SHIBS - Glycogen debranching enzyme OS=Shigella boydii serotype 4 (strain Sb227) GN=glgX PE=3 SV=1	555.0	[0..1]	[657..1]
GLGX_SHIB3 - Glycogen debranching enzyme OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=glgX PE=3...	554.0	[0..1]	[657..1]
GLGX_SHIFL - Glycogen debranching enzyme OS=Shigella flexneri GN=glgX PE=3 SV=1 GLGX_SHIF8 - Glycogen debranching enzyme OS=Shigella flexneri serotype 5b (strain 8401) GN=glgX PE=3 SV=1 gi\|24114697, gi\|... - gi\|24114697\|ref\|NP_709207.1\| glycogen debranching enzyme [Shigella flexneri 2a str. 301], gi\|2405390... gi\|30065285, gi\|... - gi\|30065285\|ref\|NP_839456.1\| glycogen debranching enzyme [Shigella flexneri 2a str. 2457T], gi\|30043...	554.0	[0..1]	[657..1]
gi\|89342483, gi\|... - gi\|89342483\|ref\|ZP_01194720.1\| Glycogen debranching enzyme GlgX [Mycobacterium flavescens PYR-GCK], ...	554.0	[0..2]	[656..11]

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Predicted Domain #1

Region A:
Residues: [1-657]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTQLAIGKPA PLGAHYDGQG VNFTLFSAHA ERVELCVFDA NGQEHRYDLP GHSGDIWHGY  60
   61 LPDARPGLRY GYRVHGPWQP AEGHRFNPAK LLIDPCARQI DGEFKDNPLL HAGHNEPDYR 120
  121 DNAAIAPKCV VVVDHYDWED DAPPRTPWGS TIIYEAHVKG LTYLHPEIPV EIRGTYKALG 180
  181 HPVMINYLKQ LGITALELLP VAQFASEPRL QRMGLSNYWG YNPVAMFALH PAYACSPETA 240
  241 LDEFRDAIKA LHKAGIEVIL DIVLNHSAEL DLDGPLFSLR GIDNRSYYWI REDGDYHNWT 300
  301 GCGNTLNLSH PAVVDYASAC LRYWVETCHV DGFRFDLAAV MGRTPEFRQD APLFTAIQNC 360
  361 PVLSQVKLIA EPWDIAPGGY QVGNFPPLFA EWNDHFRDAA RRFWLHYDLP LGAFAGRFAA 420
  421 SSDVFKRNGR LPSAAINLVT AHDGFTLRDC VCFNHKHNEA NGEENRDGTN NNYSNNHGKE 480
  481 GLGGSLDLVE RRRDSIHALL TTLLLSQGTP MLLAGDEHGH SQHGNNNAYC QDNQLTWLDW 540
  541 SQASSGLTAF TAALIHLRKR IPALVENRWW EEGDGNVRWL NRYAQPLSTD EWQNGPKQLQ 600
  601 ILLSDRFLIA INATLEVTEI VLPAGEWHAI PPFAGEDNPV ITAVWQGPAH GLCVFQR

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	92.045757
Match:	1bf2A
Description:	STRUCTURE OF PSEUDOMONAS ISOAMYLASE
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
hydrolase activity, acting on glycosyl bonds	3.1190602686215	bayes_pls_golite062009
hydrolase activity, hydrolyzing O-glycosyl compounds	2.98773241907716	bayes_pls_golite062009
hydrolase activity	2.87134532188836	bayes_pls_golite062009
catalytic activity	2.22613067995402	bayes_pls_golite062009
glycogen debranching enzyme activity	1.61342226744611	bayes_pls_golite062009
1,4-alpha-glucan branching enzyme activity	0.72343541139639	bayes_pls_golite062009
chitinase activity	0.67854733864231	bayes_pls_golite062009
glucosidase activity	0.58717079348538	bayes_pls_golite062009
hexosaminidase activity	0.16438321606077	bayes_pls_golite062009
cation transmembrane transporter activity	0.15340916113777	bayes_pls_golite062009
amylase activity	0.0914002140078796	bayes_pls_golite062009
alpha-amylase activity	0.0521244165288097	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: