YRC Public Data Repository - Protein Structure Prediction

Protein:	ASLA_ECOLI
Organism:	Escherichia coli
Length:	551 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for ASLA_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|82779021, gi\|... - gi\|82779021\|ref\|YP_405370.1\| arylsulfatase [Shigella dysenteriae Sd197], gi\|81243169\|gb\|ABB63879.1\| ...	367.0	[0..1]	[551..1]
gi\|12518665, gi\|... - gi\|15804389\|ref\|NP_290429.1\| arylsulfatase [Escherichia coli O157:H7 EDL933], gi\|12518665\|gb\|AAG5899... gi\|13364207, gi\|... - gi\|15833985\|ref\|NP_312758.1\| arylsulfatase [Escherichia coli O157:H7], gi\|13364207\|dbj\|BAB38154.1\| a... gi\|13364207, gi\|... - gi\|15833985\|ref\|NP_312758.1\| arylsulfatase [Escherichia coli O157:H7 str. Sakai], gi\|13364207\|dbj\|BA... gi\|208818746, gi... - gi\|208818746\|ref\|ZP_03259066.1\| arylsulfatase [Escherichia coli O157:H7 str. EC4042], gi\|208738869\|g... gi\|209160646, gi... - gi\|209399246\|ref\|YP_002273316.1\| arylsulfatase [Escherichia coli O157:H7 str. EC4115], gi\|209160646\|... gi\|217320752, gi... - gi\|217324531\|ref\|ZP_03440615.1\| arylsulfatase [Escherichia coli O157:H7 str. TW14588], gi\|217320752\|... gi\|168801140, gi... - gi\|189376690\|gb\|EDU95106.1\| arylsulfatase [Escherichia coli O157:H7 str. EC508], gi\|168801140\|ref\|ZP... gi\|195938087 - gi\|195938087\|ref\|ZP_03083469.1\| arylsulfatase [Escherichia coli O157:H7 str. EC4024] gi\|254595196, gi... - gi\|254795796\|ref\|YP_003080633.1\| acrylsulfatase-like enzyme [Escherichia coli O157:H7 str. TW14359],... gi\|208807165, gi... - gi\|208807165\|ref\|ZP_03249502.1\| arylsulfatase [Escherichia coli O157:H7 str. EC4206], gi\|208726966\|g... gi\|208812341, gi... - gi\|208812341\|ref\|ZP_03253670.1\| arylsulfatase [Escherichia coli O157:H7 str. EC4045], gi\|208733618\|g... gi\|189362713, gi... - gi\|189362713\|gb\|EDU81132.1\| arylsulfatase [Escherichia coli O157:H7 str. EC4486], gi\|168768077\|ref\|Z... gi\|168775653, gi... - gi\|187768864\|gb\|EDU32708.1\| arylsulfatase [Escherichia coli O157:H7 str. EC4196], gi\|168775653\|ref\|Z... gi\|189001398, gi... - gi\|189001398\|gb\|EDU70384.1\| arylsulfatase [Escherichia coli O157:H7 str. EC4076], gi\|168780695\|ref\|Z... gi\|168786634, gi... - gi\|189373327\|gb\|EDU91743.1\| arylsulfatase [Escherichia coli O157:H7 str. EC869], gi\|168786634\|ref\|ZP... gi\|168750392, gi... - gi\|188015437\|gb\|EDU53559.1\| arylsulfatase [Escherichia coli O157:H7 str. EC4113], gi\|168750392\|ref\|Z... gi\|189359385, gi... - gi\|189359385\|gb\|EDU77804.1\| arylsulfatase [Escherichia coli O157:H7 str. EC4401], gi\|168753693\|ref\|Z... gi\|209753346, gi... - pir\|\|E86066 arylsulfatase [imported] - Escherichia coli (strain O157:H7, substrain EDL933), pir\|\|C9...	358.0	[0..1]	[551..1]
gi\|237702808, gi... - gi\|237702808\|ref\|ZP_04533289.1\| arylsulfatase [Escherichia sp. 3_2_53FAA], gi\|226902979\|gb\|EEH89238.... gi\|117626058, gi... - gi\|117626058\|ref\|YP_859381.1\| arylsulfatase-like enzyme [Escherichia coli APEC O1], gi\|115515182\|gb\|... gi\|91213321, gi\|... - gi\|91213321\|ref\|YP_543307.1\| arylsulfatase [Escherichia coli UTI89], gi\|91074895\|gb\|ABE09776.1\| aryl... gi\|218367633, gi... - gi\|218560864\|ref\|YP_002393777.1\| arylsulfatase-like enzyme [Escherichia coli S88], gi\|218367633\|emb\|... gi\|26250538, gi\|... - gi\|26250538\|ref\|NP_756578.1\| arylsulfatase [Escherichia coli CFT073], gi\|26110968\|gb\|AAN83152.1\|AE01... gi\|227888617, gi... - gi\|227888617\|ref\|ZP_04006422.1\| arylsulfatase [Escherichia coli 83972], gi\|227834456\|gb\|EEJ44922.1\| ...	358.0	[0..1]	[551..1]
gi\|191174275, gi... - gi\|75236890\|ref\|ZP_00720959.1\| COG3119: Arylsulfatase A and related enzymes [Escherichia coli F11], ... gi\|110644124, gi... - gi\|110644124\|ref\|YP_671854.1\| arylsulfatase [Escherichia coli 536], gi\|110345716\|gb\|ABG71953.1\| aryl... gi\|215267199, gi... - gi\|215489127\|ref\|YP_002331558.1\| acrylsulfatase-like enzyme [Escherichia coli O127:H6 str. E2348/69]...	357.0	[0..1]	[551..1]
gi\|83585950, gi\|... - gi\|83585950\|ref\|ZP_00924589.1\| COG3119: Arylsulfatase A and related enzymes [Escherichia coli 101-1]...	351.0	[0..21]	[551..1]
gi\|19916617, gi\|... - gi\|20091471\|ref\|NP_617546.1\| arylsulfatase [Methanosarcina acetivorans C2A], gi\|19916617\|gb\|AAM06026...	336.0	[0..82]	[550..41]
gi\|37200862, gi\|... - gi\|37676321\|ref\|NP_936717.1\| arylsulfatase A [Vibrio vulnificus YJ016], gi\|37200862\|dbj\|BAC96687.1\| ...	331.0	[0..27]	[550..18]
gi\|145391 - gi\|145391\|gb\|AAC32036.1\| putative arylsulfatase [Escherichia coli]	328.0	[0..1]	[463..1]
gi\|21227994, gi\|... - gi\|21227994\|ref\|NP_633916.1\| arylsulfatase [Methanosarcina mazei Go1], gi\|20906422\|gb\|AAM31588.1\| Ar...	327.0	[0..83]	[550..3]
gi\|13474564, gi\|... - gi\|14025318\|dbj\|BAB51919.1\| arylsulfatase [Mesorhizobium loti MAFF303099], gi\|13474564\|ref\|NP_106133...	325.0	[0..77]	[550..47]

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Predicted Domain #1

Region A:
Residues: [1-80]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEFSFSPKRL VVAVAAALPL MASAADTPST ATARKGFAGY DHPNQYLVKP ATTIADNMMP  60
   61 VMQHPAQDKE TQQKLAELEK

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

		MCM Score	SCOP Match	SCOP Description
View	Download	0.461	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.459	a.46.2	Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain
View	Download	0.423	a.17.1	p8-MTCP1
View	Download	0.411	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.368	a.140.1	LEM domain

Predicted Domain #2

Region A:
Residues: [81-551]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KTGKKPNVVV FLLDDVGWMD VGFNGGGVAV GNPTPDIDAV ASQGLILTSA YSQPSSSPTR  60
   61 ATILTGQYSI HHGILMPPMY GQPGGLQGLT TLPQLLHDQG YVTQAIGKWH MGENKESQPQ 120
  121 NVGFDDFRGF NSVSDMYTEW RDVHVNPEVA LSPDRSEYIK QLPFSKDDVH AVRGGEQQAI 180
  181 ADITPKYMED LDQRWMDYGV KFLDKMAKSD KPFFLYYGTR GCHFDNYPNA KYAGSSPART 240
  241 SYGDCMVEMN DVFANLYKTL EKNGQLDNTL IVFTSDNGPE AEVPPHGRTP FRGAKGSTWE 300
  301 GGVRVPTFVY WKGMIQPRKS DGIVDLADLF PTALDLAGHP GAKVANLVPK TTFIDGVDQT 360
  361 SFFLGTNGQS NRKAEHYFLN GKLAAVRMDE FKYHVLIQQP YAYTQSGYQG GFTGTVMQTA 420
  421 GSSVFNLYTD PQESDSIGVR HIPMGVPLQT EMHAYMEILK KYPPRAQIKS D

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	67.69897
Match:	1fsuA
Description:	4-SULFATASE (HUMAN)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
sulfuric ester hydrolase activity	3.86330883060982	bayes_pls_golite062009
catalytic activity	2.31328968099128	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	1.93112755270071	bayes_pls_golite062009
steryl-sulfatase activity	1.80539840111082	bayes_pls_golite062009
binding	1.56588704144198	bayes_pls_golite062009
arylsulfatase activity	1.46195208030663	bayes_pls_golite062009
protein binding	1.23585557238219	bayes_pls_golite062009
hydrolase activity	1.14185555771205	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: