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View Structure Prediction Details

Protein: PUF60
Organism: Homo sapiens
Length: 559 amino acids
Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PUF60.

Description E-value Query
Range
Subject
Range
gi|109087692 - gi|109087692|ref|XP_001087975.1| PREDICTED: similar to fuse-binding protein-interacting repressor is...
238.0 [0..1] [559..1]
PUF60_PONPY - Poly(U)-binding-splicing factor PUF60 - Pongo pygmaeus (Orangutan)
PUF60_PONAB - Poly(U)-binding-splicing factor PUF60 OS=Pongo abelii GN=PUF60 PE=2 SV=1
237.0 [0..1] [559..1]
gi|73974700 - gi|73974700|ref|XP_848710.1| PREDICTED: similar to fuse-binding protein-interacting repressor isofor...
237.0 [0..1] [559..1]
PUF60_MOUSE - Poly(U)-binding-splicing factor PUF60 OS=Mus musculus GN=Puf60 PE=1 SV=2
PUF60_RAT - Poly(U)-binding-splicing factor PUF60 OS=Rattus norvegicus GN=Puf60 PE=2 SV=2
236.0 [0..1] [559..1]
gi|6176532 - gi|6176532|gb|AAF05605.1|AF190744_1 poly-U binding splicing factor PUF60 [Homo sapiens]
236.0 [0..4] [559..1]
gi|76677817, gi|... - gi|76677817|ref|NP_082640.1| poly-U binding splicing factor 60 isoform a [Mus musculus], gi|74203983...
234.0 [0..1] [559..1]

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Predicted Domain #1
Region A:
Residues: [1-128]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MATATIALQV NGQQGGGSEP AAAAAVVAAG DKWKPPQGTD SIKMENGQST AAKLGLPPLT  60
   61 PEQQEALQKA KKYAMEQSIK SVLVKQTIAH QQQQLTNLQM AAVTMGFGDP LSPLQSMAAQ 120
  121 RQRALAIM

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

    MCM
Score
SCOP
Match
SCOP Description
View Download 0.607 a.7.1 Spectrin repeat
View Download 0.578 a.47.2 t-snare proteins
View Download 0.530 a.7.1 Spectrin repeat
View Download 0.482 a.7.2 Enzyme IIa from lactose specific PTS, IIa-lac
View Download 0.461 a.24.9 alpha-catenin/vinculin
View Download 0.344 a.7.1 Spectrin repeat
View Download 0.313 a.24.9 alpha-catenin/vinculin
View Download 0.302 a.47.2 t-snare proteins
View Download 0.284 a.7.1 Spectrin repeat
View Download 0.264 a.216.1 Description not found.

Predicted Domain #2
Region A:
Residues: [129-317]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 CRVYVGSIYY ELGEDTIRQA FAPFGPIKSI DMSWDSVTMK HKGFAFVEYE VPEAAQLALE  60
   61 QMNSVMLGGR NIKVGRPSNI GQAQPIIDQL AEEARAFNRI YVASVHQDLS DDDIKSVFEA 120
  121 FGKIKSCTLA RDPTTGKHKG YGFIEYEKAQ SSQDAVSSMN LFDLGGQYLR VGKAVTPPMP 180
  181 LLTPATPGG

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 34.221849
Match: 1pgzA
Description: Crystal Structure of UP1 Complexed With d(TTAGGGTTAG(6-MI)G); A Human Telomeric Repeat Containing 6-methyl-8-(2-deoxy-beta-ribofuranosyl)isoxanthopteridine (6-MI)
Matching Structure (courtesy of the PDB):

Predicted Domain #3
Region A:
Residues: [318-431]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LPPAAAVAAA AATAKITAQE AVAGAAVLGT LGTPGLVSPA LTLAQPLGTL PQAVMAAQAP  60
   61 GVITGVTPAR PPIPVTIPSV GVVNPILASP PTLGLLEPKK EKEEEELFPE SERP

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

    MCM
Score
SCOP
Match
SCOP Description
View Download 0.545 a.23.1 HSC20 (HSCB), C-terminal oligomerisation domain
View Download 0.521 a.47.2 t-snare proteins
View Download 0.459 a.7.2 Enzyme IIa from lactose specific PTS, IIa-lac
View Download 0.443 a.118.8 TPR-like
View Download 0.372 d.370.1 Description not found.
View Download 0.297 a.23.1 HSC20 (HSCB), C-terminal oligomerisation domain
View Download 0.292 a.24.13 Domain of the SRP/SRP receptor G-proteins
View Download 0.289 a.24.10 Histidine-containing phosphotransfer domain, HPT domain
View Download 0.276 a.47.2 t-snare proteins

Predicted Domain #4
Region A:
Residues: [432-559]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EMLSEQEHMS ISGSSARHMV MQKLLRKQES TVMVLRNMVD PKDIDDDLEG EVTEECGKFG  60
   61 AVNRVIIYQE KQGEEEDAEI IVKIFVEFSI ASETHKAIQA LNGRWFAGRK VVAEVYDQER 120
  121 FDNSDLSA

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 4.69897
Match: 1jmtA
Description: U2AF35 (35 KDa subunit)
Matching Structure (courtesy of the PDB):

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle