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View Structure Prediction Details

Protein: MSB4
Organism: Saccharomyces cerevisiae
Length: 492 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MSB4.

Description E-value Query
Range
Subject
Range
MSB4 - GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud ...
MSB4_YEAST - GTPase-activating protein MSB4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MSB4 PE=1...
0.0 [1..492] [1..492]
gi|3327166 - gi|3327166|dbj|BAA31651.1| KIAA0676 protein [Homo sapiens]
2.0E-73 [7..490] [392..819]
gi|7507776 - gi|7507776|pir||T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans
3.0E-68 [27..490] [427..841]
gi|9790251, gi|9... - gi|988221|gb|AAA85223.1| Tbc1, gi|9790251|ref|NP_062610.1| TBC1 domain family, member 1 [Mus musculu...
1.0E-67 [16..491] [620..1095]

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Predicted Domain #1
Region A:
Residues: [1-91]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MIMSSTMSTE AALVPNESVF DTVSSFNEDD ANYSVLDLYD DDDEGDDSST VERKEILTTR  60
   61 ELEKAKAFTS LIMADPENFD RYGFSKKGYF I

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

    MCM
Score
SCOP
Match
SCOP Description
View Download 0.280 c.2.1 NAD(P)-binding Rossmann-fold domains
View Download 0.243 a.22.1 Histone-fold
View Download 0.237 a.74.1 Cyclin-like
View Download 0.299 d.68.1 Translation initiation factor IF3, C-terminal domain
View Download 0.252 d.87.1 FAD/NAD-linked reductases, dimerisation (C-terminal) domain
View Download 0.235 a.4.5 "Winged helix" DNA-binding domain
View Download 0.223 c.26.2 Adenine nucleotide alpha hydrolases-like
View Download 0.220 a.41.1 Domain of poly(ADP-ribose) polymerase
View Download 0.219 a.24.15 FAD-dependent thiol oxidase
View Download 0.211 d.6.1 Prion-like
View Download 0.200 a.118.1 ARM repeat

Predicted Domain #2
Region A:
Residues: [92-281]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SQEEYDKWWT EYNRYTERRK KKWENFLLKN KIELHNDNPL VYPARTDELS KFVRKGIPAE  60
   61 WRGNAWWYFA GGQRQLDANV GVYDRLKSDC REGAVSGKDM EAIERDLYRT FPDNIHFHKE 120
  121 SFQNGEPAII RSLRRVLMAF SVYDKTIGYC QSMNFLVGLL LLFMEEEKAF WMLVIITGKY 180
  181 LPGVYESDLE 

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [358-431]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 RIWDCLFYEE SHFLFKVALG ILKLSESEFL ESKSQKLFRQ YSSYTFGGSN DSDSTFKRLK  60
   61 NKIKTQEEAD MEIL

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 34.69897
Match: 1fkmA_
Description: Ypt/Rab-GAP domain of gyp1p
Matching Structure (courtesy of the PDB):

Predicted Domain #3
Region A:
Residues: [282-357]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GANVDQGVLV LCIKEYLPEI WSHIESSYMN GNGSTDQISG PASGEEYLCR LPTLTLCTAS  60
   61 WFMSCFVGVV PIETTL

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 34.69897
Match: 1fkmA_
Description: Ypt/Rab-GAP domain of gyp1p
Matching Structure (courtesy of the PDB):

Predicted Domain #4
Region A:
Residues: [432-492]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QVIQNFPKRL LNPNDIFEKV LMKKKVALNG ITQEKIDRGR EYVAMARNRQ RASSRPKERR  60
   61 K

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

    MCM
Score
GO
Score
GO
Term
SCOP
Match
SCOP Description
View Download 0.299 0.420 Rab GTPase activator activity a.4.5 "Winged helix" DNA-binding domain
View Download 0.299 0.054 Rab GTPase activator activity a.6.1 Putative DNA-binding domain
View Download 0.575 0.024 Rab GTPase activator activity a.4.8 Ribosomal protein S18
View Download 0.339 0.014 Rab GTPase activator activity a.60.3 C-terminal domain of RNA polymerase alpha subunit
View Download 0.304 N/A N/A g.58.1 Pheromone ER-23
View Download 0.282 N/A N/A a.4.1 Homeodomain-like
View Download 0.267 N/A N/A a.60.4 DNA repair protein Rad51, N-terminal domain
View Download 0.266 N/A N/A g.57.1 Serine proteinase inhibitor lekti
View Download 0.261 N/A N/A a.4.8 Ribosomal protein S18
View Download 0.260 N/A N/A a.4.5 "Winged helix" DNA-binding domain
View Download 0.258 N/A N/A a.23.5 Hemolysin expression modulating protein HHA
View Download 0.257 N/A N/A a.4.1 Homeodomain-like
View Download 0.247 N/A N/A a.4.5 "Winged helix" DNA-binding domain
View Download 0.233 N/A N/A a.4.1 Homeodomain-like
View Download 0.218 N/A N/A a.60.3 C-terminal domain of RNA polymerase alpha subunit
View Download 0.207 N/A N/A a.4.1 Homeodomain-like
View Download 0.207 N/A N/A d.34.1 DNA-binding domain of Mlu1-box binding protein MBP1


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