YRC Public Data Repository - Protein Structure Prediction

Protein:	BRF1
Organism:	Saccharomyces cerevisiae
Length:	596 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for BRF1.

Description	E-value	Query Range	Subject Range
TF3B_YEAST - Transcription factor IIIB 70 kDa subunit OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN... BRF1 - TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor...	0.0	[1..596]	[1..596]
TF3B_KLULA - Transcription factor IIIB 70 kDa subunit OS=Kluyveromyces lactis GN=TDS4 PE=3 SV=2 TF3B_KLULA - Transcription factor IIIB 70 kDa subunit OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM ...	0.0	[4..595]	[12..553]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[4..595]	[12..553]
gi\|238880004 - gi\|238880004\|gb\|EEQ43642.1\| hypothetical protein CAWG_01886 [Candida albicans WO-1] TF3B_CANAL - Transcription factor IIIB 70 kDa subunit OS=Candida albicans GN=TDS4 PE=3 SV=1 TF3B_CANAL - Transcription factor IIIB 70 kDa subunit OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=TDS4...	4.0E-95	[4..594]	[10..546]
gi\|8099214 - gi\|8099214\|gb\|AAF72066.1\|AF262375_1 RNA polymerase III transcription factor BRF [Drosophila melanoga...	9.0E-82	[4..594]	[7..531]
brf1 - transcription factor TFIIIB complex subunit Brf1 TF3B_SCHPO - Transcription factor IIIB 60 kDa subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=b...	4.0E-71	[4..595]	[11..495]
gi\|15232654, gi\|... - gi\|6478939\|gb\|AAF14044.1\|AC011436_28 putative transcription factor [Arabidopsis thaliana], gi\|152326...	2.0E-67	[182..595]	[78..517]

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Predicted Domain #1

Region A:
Residues: [1-61]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPVCKNCHGT EFERDLSNAN NDLVCKACGV VSEDNPIVSE VTFGETSAGA AVVQGSFIGA  60
   61 G

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	6.69897
Match:	1dl6A_
Description:	Transcription inititiation factor TFIIB, N-terminal domain
Matching Structure (courtesy of the PDB):

Predicted Domain #2

Region A:
Residues: [62-175]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QSHAAFGGSS ALESREATLN NARRKLRAVS YALHIPEYIT DAAFQWYKLA LANNFVQGRR  60
   61 SQNVIASCLY VACRKEKTHH MLIDFSSRLQ VSVYSIGATF LKMVKKLHIT ELPL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	189.154902
Match:	1d3uB_
Description:	Transcription factor IIB (TFIIB), core domain
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [176-281]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 ADPSLFIQHF AEKLDLADKK IKVVKDAVKL AQRMSKDWMF EGRRPAGIAG ACILLACRMN  60
   61 NLRRTHTEIV AVSHVAEETL QQRLNEFKNT KAAKLSVQKF RENDVE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	189.154902
Match:	1d3uB_
Description:	Transcription factor IIB (TFIIB), core domain
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [282-596]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DGEARPPSFV KNRKKERKIK DSLDKEEMFQ TSEEALNKNP ILTQVLGEQE LSSKEVLFYL  60
   61 KQFSERRARV VERIKATNGI DGENIYHEGS ENETRKRKLS EVSIQNEHVE GEDKETEGTE 120
  121 EKVKKVKTKT SEEKKENESG HFQDAIDGYS LETDPYCPRN LHLLPTTDTY LSKVSDDPDN 180
  181 LEDVDDEELN AHLLNEEASK LKERIWIGLN ADFLLEQESK RLKQEADIAT GNTSVKKKRT 240
  241 RRRNNTRSDE PTKTVDAAAA IGLMSDLQDK SGLHAALKAA EESGDFTTAD SVKNMLQKAS 300
  301 FSKKINYDAI DGLFR

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits