YRC Public Data Repository - Protein Structure Prediction

Protein:	HNT2
Organism:	Saccharomyces cerevisiae
Length:	217 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for HNT2.

Description	E-value	Query Range	Subject Range
HNT2 - Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the ... gi\|190404753 - gi\|190404753\|gb\|EDV08020.1\| hit family protein 2 [Saccharomyces cerevisiae RM11-1a] gi\|151942277 - gi\|151942277\|gb\|EDN60633.1\| histidine triad nucleotide-binding protein [Saccharomyces cerevisiae YJM...	8.0E-67	[1..217]	[1..217]
aph1 - bis(5'-nucleosidyl)-tetraphosphatase APH1_SCHPO - Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24...	6.0E-40	[12..205]	[1..177]
HIT_BACSU - Protein hit OS=Bacillus subtilis (strain 168) GN=hit PE=1 SV=1 gi\|221322363 - gi\|221322363\|ref\|ZP_03603657.1\| Hit-like protein [Bacillus subtilis subsp. subtilis str. SMY] HIT_BACSU - Protein hit OS=Bacillus subtilis GN=hit PE=1 SV=1 gi\|221313165 - gi\|221313165\|ref\|ZP_03594970.1\| Hit-like protein [Bacillus subtilis subsp. subtilis str. NCIB 3610] gi\|221318088 - gi\|221318088\|ref\|ZP_03599382.1\| Hit-like protein [Bacillus subtilis subsp. subtilis str. JH642]	4.0E-36	[13..165]	[4..143]
gi\|25288050 - pir\|\|E83798 Hit-like protein involved in cell-cycle regulation hit [imported] - Bacillus halodurans ... gi\|10173805, gi\|... - gi\|15613752\|ref\|NP_242055.1\| Hit-like protein involved in cell-cycle regulation [Bacillus halodurans...	3.0E-35	[10..149]	[1..142]
gi\|7434504 - pir\|\|F71224 hypothetical protein PH0057 - Pyrococcus horikoshii gi\|14590010, gi\|... - gi\|3256442\|dbj\|BAA29125.1\| 168aa long hypothetical protein [Pyrococcus horikoshii OT3], gi\|14590010\|...	7.0E-34	[12..158]	[17..166]
gi\|7515016, gi\|5... - pir\|\|E75192 hit family protein (hit). PAB2301 - Pyrococcus abyssi (strain Orsay), gi\|5457497\|emb\|CA... gi\|14520282, gi\|... - gi\|5457497\|emb\|CAB48988.1\| hit histidine triad protein [Pyrococcus abyssi GE5], gi\|14520282\|ref\|NP_1...	7.0E-34	[10..158]	[32..183]
gi\|254872684 - gi\|254872684\|ref\|ZP_05245394.1\| histidine triad (HIT) protein [Listeria monocytogenes FSL N1-017] gi\|254878167 - gi\|254878167\|ref\|ZP_05250877.1\| histidine triad (HIT) protein [Listeria monocytogenes FSL J2-071] gi\|254992113 - gi\|254992113\|ref\|ZP_05274303.1\| histidine triad (HIT) protein [Listeria monocytogenes FSL J2-064] gi\|255025822 - gi\|255025822\|ref\|ZP_05297808.1\| histidine triad (HIT) protein [Listeria monocytogenes FSL J2-003] gi\|255028658 - gi\|255028658\|ref\|ZP_05300609.1\| histidine triad (HIT) protein [Listeria monocytogenes LO28] gi\|46908450, gi\|... - gi\|46908450\|ref\|YP_014839.1\| HIT family protein [Listeria monocytogenes str. 4b F2365], gi\|46881721\|... gi\|153185079, gi... - gi\|254899082\|ref\|ZP_05259006.1\| histidine triad (HIT) protein [Listeria monocytogenes J0161], gi\|153... gi\|153197037, gi... - gi\|254831347\|ref\|ZP_05236002.1\| histidine triad (HIT) protein [Listeria monocytogenes 10403S], gi\|15... gi\|16804255 - gi\|16804255\|ref\|NP_465740.1\| hypothetical protein lmo2216 [Listeria monocytogenes EGD-e] gi\|254912777, gi... - gi\|254912777\|ref\|ZP_05262789.1\| histidine triad (HIT) protein [Listeria monocytogenes J2818], gi\|153... gi\|47014280, gi\|... - gi\|47097357\|ref\|ZP_00234911.1\| HIT family protein [Listeria monocytogenes str. 1/2a F6854], gi\|47014... gi\|254826895, gi... - gi\|254826895\|ref\|ZP_05231582.1\| histidine triad (HIT) protein [Listeria monocytogenes FSL N3-165], g... gi\|47093741, gi\|... - gi\|47093741\|ref\|ZP_00231492.1\| HIT family protein [Listeria monocytogenes str. 4b H7858], gi\|4701789... gi\|133732407, gi... - gi\|254937104\|ref\|ZP_05268801.1\| histidine triad (HIT) protein [Listeria monocytogenes F6900], gi\|153... gi\|25288048, gi\|... - pir\|\|AH1351 histidine triad (HIT) protein homolog lmo2216 [imported] - Listeria monocytogenes (strai... gi\|254931671, gi... - gi\|254931671\|ref\|ZP_05265030.1\| histidine triad (HIT) protein [Listeria monocytogenes HPB2262], gi\|1... gi\|224500984 - gi\|224500984\|ref\|ZP_03669291.1\| HIT family protein [Listeria monocytogenes FSL R2-561] gi\|224498961 - gi\|224498961\|ref\|ZP_03667310.1\| HIT family protein [Listeria monocytogenes Finland 1988] gi\|226224824, gi... - gi\|226224824\|ref\|YP_002758931.1\| histidine triad (HIT) protein [Listeria monocytogenes Clip81459], g... gi\|254825220 - gi\|254825220\|ref\|ZP_05230221.1\| histidine triad (HIT) protein [Listeria monocytogenes FSL J1-194] gi\|254853340 - gi\|254853340\|ref\|ZP_05242688.1\| histidine triad (HIT) protein [Listeria monocytogenes FSL R2-503]	2.0E-32	[14..163]	[2..138]
gi\|18976380, gi\|... - gi\|18976380\|ref\|NP_577737.1\| hit family protein [Pyrococcus furiosus DSM 3638], gi\|18891904\|gb\|AAL80...	3.0E-32	[10..153]	[15..161]
Y866_METJA - Uncharacterized HIT-like protein MJ0866 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 26... Y866_METJA - Uncharacterized HIT-like protein MJ0866 OS=Methanocaldococcus jannaschii GN=MJ0866 PE=4 SV=2	5.0E-32	[16..120]	[2..107]

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Predicted Domain #1

Region A:
Residues: [1-160]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MILSKTKKPK SMNKPIYFSK FLVTEQVFYK SKYTYALVNL KPIVPGHVLI VPLRTTVLNL  60
   61 SDLTMPESQD YFKTLQLIHR FIKWQYKADS INVAIQDGPE AGQSVPHLHT HIIPRYKINN 120
  121 VGDLIYDKLD HWDGNGTLTD WQGRRDEYLG VGGRQARKNN

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	212.512579
Match:	1fhi__
Description:	FHIT (fragile histidine triad protein)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
binding	0.967106554167137	bayes_pls_golite062009
nucleic acid binding	0.724655038971066	bayes_pls_golite062009
catalytic activity	0.096119422680995	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [161-217]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 STSATVDGDE LSQGPNVLKP DSQRKVRALT EMKKEAEDLQ ARLEEFVSSD PGLTQWL

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

		MCM Score	SCOP Match	SCOP Description
View	Download	0.688	a.8.1	Bacterial immunoglobulin/albumin-binding domains
View	Download	0.334	a.52.1	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin
View	Download	0.271	a.157.1	Skp1 dimerisation domain-like
View	Download	0.543	a.179.1	Replisome organizer (g39p helicase loader/inhibitor protein)
View	Download	0.413	g.53.1	TAZ domain
View	Download	0.266	a.5.2	UBA-like
View	Download	0.263	a.56.1	CO dehydrogenase ISP C-domain like
View	Download	0.259	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.238	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.236	a.4.9	Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3
View	Download	0.236	a.4.1	Homeodomain-like
View	Download	0.233	d.58.3	Protease propeptides/inhibitors
View	Download	0.229	a.65.1	Annexin
View	Download	0.225	a.46.2	Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain
View	Download	0.224	a.23.5	Hemolysin expression modulating protein HHA
View	Download	0.215	a.44.1	Disulphide-bond formation facilitator (DSBA), insertion domain
View	Download	0.203	d.50.3	PI-Pfui intein middle domain

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: