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View Structure Prediction Details

Protein: GCS1
Organism: Saccharomyces cerevisiae
Length: 352 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for GCS1.

Description E-value Query
Range
Subject
Range
GCS1 - ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares ...
GCS1_YEAST - ADP-ribosylation factor GTPase-activating protein GCS1 OS=Saccharomyces cerevisiae (strain ATCC 2045...
0.0 [1..352] [1..352]
ARFGAP1 - ADP-ribosylation factor GTPase activating protein 1
4.0E-80 [8..352] [4..332]
gi|10441356 - gi|10441356|gb|AAG17006.1|AF184146_1 ARF GAP-like zinc finger-containing protein ZIGA2 [Arabidopsis ...
3.0E-78 [12..351] [5..394]
gi|11691875 - gi|11691875|emb|CAC18721.1| ADP-ribosylation factor 1 GTPase activating protein [Mus musculus]
1.0E-75 [8..352] [4..332]
ARFG1_RAT - ADP-ribosylation factor GTPase-activating protein 1 OS=Rattus norvegicus GN=Arfgap1 PE=1 SV=1
2.0E-75 [8..352] [4..332]
Gap69C-PA - The gene GTPase-activating protein 69C is referred to in FlyBase by the symbol Dmel\Gap69C (CG4237, ...
gi|220958084, gi... - gi|220958084|gb|ACL91585.1| Gap69C-PA [synthetic construct], gi|220948710|gb|ACL86898.1| Gap69C-PA [...
3.0E-73 [8..350] [4..352]
gi|7507776 - gi|7507776|pir||T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans
6.0E-57 [1..284] [1..283]

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Predicted Domain #1
Region A:
Residues: [1-201]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDWKVDPDT RRRLLQLQKI GANKKCMDCG APNPQWATPK FGAFICLECA GIHRGLGVHI  60
   61 SFVRSITMDQ FKPEELLRME KGGNEPLTEW FKSHNIDLSL PQKVKYDNPV AEDYKEKLTC 120
  121 LCEDRVFEER EHLDFDASKL SATSQTAASA TPGVAQSREG TPLENRRSAT PANSSNGANF 180
  181 QKEKNEAYFA ELGKKNQSRP D

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 187.221849
Match: 1dcqA_
Description: Pyk2-associated protein beta; Pyk2-associated protein beta ARF-GAP domain
Matching Structure (courtesy of the PDB):

Predicted Domain #2
Region A:
Residues: [202-254]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 HLPPSQGGKY QGFGSTPAKP PQERSAGSSN TLSLENFQAD PLGTLSRGWG LFS

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

    MCM
Score
SCOP
Match
SCOP Description
View Download 0.233 a.4.1 Homeodomain-like
View Download 0.268 a.155.1 H-NS histone-like proteins
View Download 0.229 a.60.3 C-terminal domain of RNA polymerase alpha subunit
View Download 0.310 a.159.2 FF domain
View Download 0.310 a.159.2 FF domain
View Download 0.268 a.155.1 H-NS histone-like proteins
View Download 0.233 a.4.1 Homeodomain-like
View Download 0.229 a.60.3 C-terminal domain of RNA polymerase alpha subunit

Predicted Domain #3
Region A:
Residues: [255-352]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SAVTKSFEDV NETVIKPHVQ QWQSGELSEE TKRAAAQFGQ KFQETSSYGF QAFSNFTKNF  60
   61 NGNAEDSSTA GNTTHTEYQK IDNNDKKNEQ DEDKWDDF

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

    MCM
Score
GO
Score
GO
Term
SCOP
Match
SCOP Description
View Download 0.652 0.466 ER to Golgi vesicle-mediated transport a.118.8 TPR-like
View Download 0.563 0.101 retrograde vesicle-mediated transport, Golgi to ER a.118.1 ARM repeat
View Download 0.524 0.033 retrograde vesicle-mediated transport, Golgi to ER a.118.9 ENTH/VHS domain
View Download 0.564 0.023 retrograde vesicle-mediated transport, Golgi to ER a.46.2 Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain
View Download 0.742 N/A N/A a.29.4 RecG, N-terminal domain
View Download 0.742 N/A N/A a.29.4 RecG, N-terminal domain
View Download 0.652 N/A N/A a.118.8 TPR-like
View Download 0.564 N/A N/A a.46.2 Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain
View Download 0.563 N/A N/A a.118.1 ARM repeat
View Download 0.524 N/A N/A a.118.9 ENTH/VHS domain
View Download 0.510 N/A N/A a.60.9 lambda integrase-like, N-terminal domain
View Download 0.414 N/A N/A a.118.11 Cytochrome c oxidase subunit E
View Download 0.406 N/A N/A a.66.1 Transducin (alpha subunit), insertion domain
View Download 0.383 N/A N/A a.7.3 Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain
View Download 0.377 N/A N/A a.24.10 Histidine-containing phosphotransfer domain, HPT domain
View Download 0.369 N/A N/A a.52.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin
View Download 0.364 N/A N/A a.118.8 TPR-like
View Download 0.363 N/A N/A a.7.6 Ribosomal protein S20
View Download 0.353 N/A N/A a.23.3 Methane monooxygenase hydrolase, gamma subunit
View Download 0.353 N/A N/A a.46.2 Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain
View Download 0.337 N/A N/A a.118.1 ARM repeat
View Download 0.330 N/A N/A a.181.1 Antibiotic binding domain of TipA-like multidrug resistance regulators
View Download 0.327 N/A N/A a.16.1 S15/NS1 RNA-binding domain
View Download 0.324 N/A N/A a.45.1 Glutathione S-transferase (GST), C-terminal domain
View Download 0.312 N/A N/A a.27.1 Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases
View Download 0.312 N/A N/A a.4.1 Homeodomain-like
View Download 0.308 N/A N/A a.24.11 Bacterial GAP domain
View Download 0.291 N/A N/A c.114.1 YchN-like
View Download 0.266 N/A N/A a.45.1 Glutathione S-transferase (GST), C-terminal domain
View Download 0.266 N/A N/A f.14.1 Voltage-gated potassium channels
View Download 0.264 N/A N/A a.52.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin
View Download 0.256 N/A N/A a.24.3 Cytochromes
View Download 0.251 N/A N/A a.81.1 N-terminal domain of DnaB helicase
View Download 0.234 N/A N/A a.24.7 FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP)
View Download 0.221 N/A N/A a.49.1 C-terminal domain of B transposition protein
View Download 0.220 N/A N/A a.118.13 Arp2/3 complex 16 kDa subunit ARPC5
View Download 0.216 N/A N/A a.77.1 DEATH domain
View Download 0.215 N/A N/A a.24.10 Histidine-containing phosphotransfer domain, HPT domain
View Download 0.211 N/A N/A a.24.15 FAD-dependent thiol oxidase
View Download 0.208 N/A N/A a.71.1 Endoplasmic reticulum protein ERP29, C-domain


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