YRC Public Data Repository - Protein Structure Prediction

Protein:	LYS20
Organism:	Saccharomyces cerevisiae
Length:	428 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for LYS20.

Description	E-value	Query Range	Subject Range
LE12_METJA, LEU1... - 2-isopropylmalate synthase 2 OS=Methanocaldococcus jannaschii GN=leuA2 PE=3 SV=1, (Q58595) 2-isoprop... LEU1_METJA - 2-isopropylmalate synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / ...	0.0	[5..426]	[6..429]
gi\|3282740, gi\|1... - gi\|3282740\|gb\|AAD12600.1\| 2-isopropylmalate synthase [Buchnera aphidicola], gi\|10954458\|ref\|NP_04718... LEU1_BUCDN - 2-isopropylmalate synthase OS=Buchnera aphidicola subsp. Diuraphis noxia GN=leuA PE=3 SV=1	0.0	[22..423]	[4..413]
LEU12_ARATH - 2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana GN=IPMS2 PE=1 SV=1	0.0	[3..422]	[62..507]
gi\|89104762, gi\|... - gi\|89104762\|ref\|ZP_01177296.1\| COG0119: Isopropylmalate/homocitrate/citramalate synthases [Yersinia ... LEU1_YERPA - 2-isopropylmalate synthase OS=Yersinia pestis bv. Antiqua (strain Antiqua) GN=leuA PE=3 SV=1 gi\|165992051, gi... - gi\|166008712\|ref\|ZP_02229610.1\| 2-isopropylmalate synthase [Yersinia pestis biovar Antiqua str. E197... LEU1_YERPS - 2-isopropylmalate synthase OS=Yersinia pseudotuberculosis GN=leuA PE=3 SV=1 gi\|165919820, gi... - gi\|165928209\|ref\|ZP_02224041.1\| 2-isopropylmalate synthase [Yersinia pestis biovar Orientalis str. F... gi\|167049327, gi... - gi\|167401302\|ref\|ZP_02306802.1\| 2-isopropylmalate synthase [Yersinia pestis biovar Antiqua str. UG05... gi\|166206878, gi... - gi\|166212132\|ref\|ZP_02238167.1\| 2-isopropylmalate synthase [Yersinia pestis biovar Antiqua str. B420... gi\|45443381, gi\|... - gi\|45443381\|ref\|NP_994920.1\| 2-isopropylmalate synthase [Yersinia pestis biovar Microtus str. 91001]... gi\|16120862, gi\|... - gi\|218927730\|ref\|YP_002345605.1\| 2-isopropylmalate synthase [Yersinia pestis CO92], gi\|16120862\|ref\|... LEU1_YERPS - 2-isopropylmalate synthase OS=Yersinia pseudotuberculosis serotype I (strain IP32953) GN=leuA PE=3 S... LEU1_YERPG - 2-isopropylmalate synthase OS=Yersinia pestis bv. Antiqua (strain Angola) GN=leuA PE=3 SV=1 LEU1_YERP3 - 2-isopropylmalate synthase OS=Yersinia pseudotuberculosis serotype O:1b (strain IP 31758) GN=leuA PE... LEU1_YERPN - 2-isopropylmalate synthase OS=Yersinia pestis bv. Antiqua (strain Nepal516) GN=leuA PE=3 SV=1 LEU1_YERPE - 2-isopropylmalate synthase OS=Yersinia pestis GN=leuA PE=3 SV=1 gi\|167469504 - gi\|167469504\|ref\|ZP_02334208.1\| 2-isopropylmalate synthase [Yersinia pestis FV-1] gi\|166960132, gi... - gi\|167421995\|ref\|ZP_02313748.1\| 2-isopropylmalate synthase [Yersinia pestis biovar Orientalis str. M... LEU1_YERPB - 2-isopropylmalate synthase OS=Yersinia pseudotuberculosis serotype IB (strain PB1/+) GN=leuA PE=3 SV... gi\|149289839, gi... - gi\|150260431\|ref\|ZP_01917159.1\| 2-isopropylmalate synthase [Yersinia pestis CA88-4125], gi\|149289839... LEU1_YERPY - 2-isopropylmalate synthase OS=Yersinia pseudotuberculosis serotype O:3 (strain YPIII) GN=leuA PE=3 S... gi\|229836878, gi... - gi\|229836878\|ref\|ZP_04457043.1\| 2-isopropylmalate synthase [Yersinia pestis Pestoides A], gi\|2297058... LEU1_YERPP - 2-isopropylmalate synthase OS=Yersinia pestis (strain Pestoides F) GN=leuA PE=3 SV=1 gi\|229840424, gi... - gi\|229840424\|ref\|ZP_04460583.1\| 2-isopropylmalate synthase [Yersinia pestis biovar Orientalis str. P... gi\|229843035, gi... - gi\|229843035\|ref\|ZP_04463185.1\| 2-isopropylmalate synthase [Yersinia pestis biovar Orientalis str. I...	0.0	[21..422]	[3..412]
LEU1A_SOLPN - 2-isopropylmalate synthase A OS=Solanum pennellii GN=IPMSA PE=2 SV=1	0.0	[9..422]	[30..468]
LEU1_BUCAI - 2-isopropylmalate synthase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) GN=leuA PE... LEU1_BUCAI - 2-isopropylmalate synthase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=leuA PE=3 SV=3 gi\|219783132\|emb... - unnamed protein product [Buchnera aphidicola], unnamed protein product [Buchnera aphidicola], unname...	0.0	[22..422]	[4..412]
LEU1_BUCAP - 2-isopropylmalate synthase OS=Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) GN=leuA PE=... gi\|219730356\|emb... - unnamed protein product [Buchnera aphidicola], unnamed protein product [Buchnera aphidicola], unname... LEU1_BUCAP - 2-isopropylmalate synthase OS=Buchnera aphidicola subsp. Schizaphis graminum GN=leuA PE=3 SV=1	0.0	[22..420]	[4..412]
LEU1_BUCRP - 2-isopropylmalate synthase OS=Buchnera aphidicola subsp. Rhopalosiphum padi GN=leuA PE=3 SV=1 gi\|899388 - gi\|899388\|emb\|CAA50615.1\| 2-isopropylmalate synthase [Buchnera aphidicola]	0.0	[22..420]	[4..412]
LYS20 - Homocitrate synthase isozyme, catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to fo... HOSC_YEAST - Homocitrate synthase, cytosolic isozyme OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=... gi\|190405178 - gi\|190405178\|gb\|EDV08445.1\| homocitrate synthase [Saccharomyces cerevisiae RM11-1a] gi\|151941823 - gi\|151941823\|gb\|EDN60179.1\| homocitrate synthase [Saccharomyces cerevisiae YJM789]	0.0	[1..428]	[1..428]

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Predicted Domain #1

Region A:
Residues: [1-261]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTAAKPNPYA AKPGDYLSNV NNFQLIDSTL REGEQFANAF FDTEKKIEIA RALDDFGVDY  60
   61 IELTSPVASE QSRKDCEAIC KLGLKAKILT HIRCHMDDAK VAVETGVDGV DVVIGTSKFL 120
  121 RQYSHGKDMN YIAKSAVEVI EFVKSKGIEI RFSSEDSFRS DLVDLLNIYK TVDKIGVNRV 180
  181 GIADTVGCAN PRQVYELIRT LKSVVSCDIE CHFHNDTGCA IANAYTALEG GARLIDVSVL 240
  241 GIGERNGITP LGGLMARMIV A

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	11.45
Match:	1euaA
Description:	KDPG aldolase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
catalytic activity	2.67031204377352	bayes_pls_golite062009
porphobilinogen synthase activity	1.24455949903754	bayes_pls_golite062009
binding	1.1388634428163	bayes_pls_golite062009
lyase activity	0.740424046054758	bayes_pls_golite062009
transferase activity, transferring aldehyde or ketonic groups	0.487442544994915	bayes_pls_golite062009
transferase activity	0.464721888799972	bayes_pls_golite062009
transaldolase activity	0.184100272651834	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [262-428]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 APDYVKSKYK LHKIRDIENL VADAVEVNIP FNNPITGFCA FTHKAGIHAK AILANPSTYE  60
   61 ILDPHDFGMK RYIHFANRLT GWNAIKARVD QLNLNLTDDQ IKEVTAKIKK LGDVRSLNID 120
  121 DVDSIIKNFH AEVSTPQVLS AKKNKKNDSD VPELATIPAA KRTKPSA

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: