YRC Public Data Repository - Protein Structure Prediction

Protein:	ECM31
Organism:	Saccharomyces cerevisiae
Length:	312 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for ECM31.

Description	E-value	Query Range	Subject Range
ECM31 - Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoiso... PANB_YEAST - 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S...	0.0	[1..312]	[1..312]
PANB2_ARATH - 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana GN=KPHMT2 ...	0.0	[20..311]	[38..328]
PANB_CLOAB - 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Clostridium acetobutylicum (strain ATCC 824 / DS... PANB_CLOAB - 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Clostridium acetobutylicum GN=panB PE=3 SV=1	0.0	[25..304]	[2..273]
PANB_EMENI - 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Emericella nidulans GN=panB PE=3 SV=1 PANB_EMENI - 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163...	0.0	[21..311]	[60..344]
PANB_BACHD - 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Bacillus halodurans GN=panB PE=3 SV=1 PANB_BACHD - 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 1...	0.0	[27..304]	[3..272]
PANB2_ORYSJ - 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativa subsp. japonica GN... gi\|125524981 - gi\|125524981\|gb\|EAY73095.1\| hypothetical protein OsI_00971 [Oryza sativa Indica Group]	0.0	[24..312]	[82..368]
PANB_BACSU - 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Bacillus subtilis (strain 168) GN=panB PE=3 SV=1 PANB_BACSU - 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Bacillus subtilis GN=panB PE=3 SV=1 gi\|221319403 - gi\|221319403\|ref\|ZP_03600697.1\| 3-methyl-2-oxobutanoate hydroxymethyltransferase [Bacillus subtilis ... gi\|221323679 - gi\|221323679\|ref\|ZP_03604973.1\| 3-methyl-2-oxobutanoate hydroxymethyltransferase [Bacillus subtilis ... gi\|221314481 - gi\|221314481\|ref\|ZP_03596286.1\| 3-methyl-2-oxobutanoate hydroxymethyltransferase [Bacillus subtilis ...	0.0	[30..304]	[6..272]
gi\|218930418, gi... - gi\|218930418\|ref\|YP_002348293.1\| 3-methyl-2-oxobutanoate hydroxymethyltransferase [Yersinia pestis C... gi\|149289193, gi... - gi\|153997556\|ref\|ZP_02022656.1\| 3-methyl-2-oxobutanoate hydroxymethyltransferase [Yersinia pestis CA... gi\|25286025 - pir\|\|AC0413 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) [imported] - Yersinia pes...	0.0	[22..296]	[1..266]

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Predicted Domain #1

Region A:
Residues: [1-312]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNIMKRQLCT SSKRFFSTAK NVVKYNTIQD IRNKYFTGTP LSMCTAYDFI TATWVNKANC  60
   61 DLLLVGDSLA MTSLGYDSTI TLSLNEFKYH VASVCRAEGS SMVVVDMPFG TFESGISDGL 120
  121 KNAIDIMKLD SKVTSVKVEV GSYTKDKYAM KFIEELCSRG IPVMAHIGLT PQKVHSLGGY 180
  181 KVQGSKSLLQ MQELYETAMQ LQKIGCWSIL IECVPHKMAQ FITSKLSVPT IGIGAGNGTS 240
  241 GQVLVISDLL GMQGDSVPKF VKQAVNMTDI ATQGLKEYIA SVEDRTFPER GTHTFKVKED 300
  301 LWNEFLSSIN EK

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	8.63
Match:	1a50A
Description:	Trp synthase alpha-subunit
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
3-methyl-2-oxobutanoate hydroxymethyltransferase activity	4.08693637310631	bayes_pls_golite062009
catalytic activity	2.59838799615646	bayes_pls_golite062009
hydroxymethyl-, formyl- and related transferase activity	2.05689968523792	bayes_pls_golite062009
transferase activity	1.07154136609184	bayes_pls_golite062009
glycine hydroxymethyltransferase activity	0.646649320021871	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.366715189574293	bayes_pls_golite062009
oxo-acid-lyase activity	0.0960362356555544	bayes_pls_golite062009
carbon-carbon lyase activity	0.0846611486307003	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: