YRC Public Data Repository - Protein Structure Prediction

Protein:	NADE_PECAS
Organism:	Pectobacterium atrosepticum SCRI1043
Length:	274 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for NADE_PECAS.

Description	E-value	Query Range	Subject Range
gi\|253773306, gi... - gi\|254288587\|ref\|YP_003054335.1\| NAD synthetase [Escherichia coli BL21(DE3)], gi\|253977894\|gb\|ACT435... gi\|242377461, gi... - gi\|251785188\|ref\|YP_002999492.1\| hypothetical protein B21_01697 [Escherichia coli BL21], gi\|24237746... gi\|254161799, gi... - gi\|254161799\|ref\|YP_003044907.1\| NAD synthetase [Escherichia coli B str. REL606], gi\|253973700\|gb\|AC... gi\|194438578, gi... - gi\|83586039\|ref\|ZP_00924677.1\| COG0171: NAD synthase [Escherichia coli 101-1], gi\|194438578\|ref\|ZP_0...	271.0	[0..1]	[274..1]
NADE_ECOK1 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O1:K1 / APEC GN=nadE PE=3 SV=1 NADE_ECOUT - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli (strain UTI89 / UPEC) GN=nadE PE=3 SV=1 gi\|226900447, gi... - gi\|237705690\|ref\|ZP_04536171.1\| NH(3)-dependent NAD(+) synthetase [Escherichia sp. 3_2_53FAA], gi\|22... NADE_ECO45 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=nadE PE=3 SV=1	271.0	[0..1]	[274..1]
NADE_ECO7I - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=nadE PE=3 SV=1 NADE_ECO55 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli (strain 55989 / EAEC) GN=nadE PE=3 SV=1 NADE_ECO81 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O81 (strain ED1a) GN=nadE PE=3 SV=1 gi\|194418241, gi... - gi\|83569656\|ref\|ZP_00921107.1\| COG0171: NAD synthase [Shigella dysenteriae 1012], gi\|194433478\|ref\|Z...	270.0	[0..1]	[274..1]
gi\|75256142, gi\|... - gi\|75256142\|ref\|ZP_00727838.1\| COG0171: NAD synthase [Escherichia coli E22], gi\|193065791\|ref\|ZP_030... gi\|75210227, gi\|... - gi\|75210227\|ref\|ZP_00710395.1\| COG0171: NAD synthase [Escherichia coli B171], gi\|194428548\|ref\|ZP_03...	270.0	[0..1]	[274..1]
gi\|75227067, gi\|... - gi\|75227067\|ref\|ZP_00713891.1\| COG0171: NAD synthase [Escherichia coli B7A], gi\|191169567\|ref\|ZP_030...	270.0	[0..1]	[274..1]
gi\|75241385, gi\|... - gi\|75241385\|ref\|ZP_00725236.1\| COG0171: NAD synthase [Escherichia coli F11], gi\|191171747\|ref\|ZP_030... NADE_ECOL5 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=nadE PE=3 SV... gi\|227885836, gi... - gi\|227885836\|ref\|ZP_04003641.1\| NAD(+) synthase [Escherichia coli 83972], gi\|227837409\|gb\|EEJ47875.1... NADE_ECOL6 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) GN=... NADE_ECOL6 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O6 GN=nadE PE=3 SV=1	270.0	[0..1]	[274..1]
gi\|15831700, gi\|... - gi\|15831700\|ref\|NP_310473.1\| NAD synthetase [Escherichia coli O157:H7 str. Sakai], gi\|13361913\|dbj\|B... gi\|208816632, gi... - gi\|208816632\|ref\|ZP_03257752.1\| NAD+ synthetase [Escherichia coli O157:H7 str. EC4045], gi\|208730975... gi\|208820140, gi... - gi\|208820140\|ref\|ZP_03260460.1\| NAD+ synthetase [Escherichia coli O157:H7 str. EC4042], gi\|208740263... NADE_ECO5E - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=nadE PE=3 SV... gi\|217318324, gi... - gi\|217328977\|ref\|ZP_03445058.1\| NAD+ synthetase [Escherichia coli O157:H7 str. TW14588], gi\|21731832... gi\|189372069, gi... - gi\|189372069\|gb\|EDU90485.1\| NAD+ synthetase [Escherichia coli O157:H7 str. EC869], gi\|168788139\|ref\|... gi\|189377569, gi... - gi\|189377569\|gb\|EDU95985.1\| NAD+ synthetase [Escherichia coli O157:H7 str. EC508], gi\|168800077\|ref\|... gi\|195937424 - gi\|195937424\|ref\|ZP_03082806.1\| NAD synthetase [Escherichia coli O157:H7 str. EC4024] gi\|208810295, gi... - gi\|208810295\|ref\|ZP_03252171.1\| NAD+ synthetase [Escherichia coli O157:H7 str. EC4206], gi\|208724811... gi\|189367472, gi... - gi\|189367472\|gb\|EDU85888.1\| NAD+ synthetase [Escherichia coli O157:H7 str. EC4501], gi\|168762194\|ref... gi\|168770504, gi... - gi\|189360607\|gb\|EDU79026.1\| NAD+ synthetase [Escherichia coli O157:H7 str. EC4486], gi\|168770504\|ref... gi\|187769486, gi... - gi\|187769486\|gb\|EDU33330.1\| NAD+ synthetase [Escherichia coli O157:H7 str. EC4196], gi\|168774957\|ref... gi\|168782158, gi... - gi\|189000295\|gb\|EDU69281.1\| NAD+ synthetase [Escherichia coli O157:H7 str. EC4076], gi\|168782158\|ref... gi\|188016246, gi... - gi\|188016246\|gb\|EDU54368.1\| NAD+ synthetase [Escherichia coli O157:H7 str. EC4113], gi\|168749442\|ref... gi\|168756744, gi... - gi\|189356171\|gb\|EDU74590.1\| NAD+ synthetase [Escherichia coli O157:H7 str. EC4401], gi\|168756744\|ref... gi\|254793358, gi... - gi\|254793358\|ref\|YP_003078195.1\| NAD synthetase, NH3/glutamine-dependent [Escherichia coli O157:H7 s... NADE_ECO57 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O157:H7 GN=nadE PE=3 SV=1 gi\|209768646, gi... - pir\|\|B85783 NAD synthetase, prefers NH3 over glutamine [imported] - Escherichia coli (strain O157:H7... gi\|15802151, gi\|... - gi\|15802151\|ref\|NP_288173.1\| NAD synthetase [Escherichia coli O157:H7 EDL933], gi\|12515759\|gb\|AAG567...	269.0	[0..1]	[274..1]
NADE_ECO24 - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=nadE PE=3 ... NADE_ECOSE - NH(3)-dependent NAD(+) synthetase OS=Escherichia coli (strain SE11) GN=nadE PE=3 SV=1 NADE_SHISS - NH(3)-dependent NAD(+) synthetase OS=Shigella sonnei (strain Ss046) GN=nadE PE=3 SV=1	269.0	[0..1]	[274..1]
NADE_SHIDS - NH(3)-dependent NAD(+) synthetase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=nadE PE=3 SV=...	269.0	[0..1]	[274..1]
gi\|192957766, gi... - gi\|75236791\|ref\|ZP_00720862.1\| COG0171: NAD synthase [Escherichia coli E110019], gi\|193068971\|ref\|ZP...	269.0	[0..1]	[274..1]

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Predicted Domain #1

Region A:
Residues: [1-274]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLQNDIITA LGVKSSIDPA QEIRVSVDFL KNYLNAHPFI TSLVLGISGG QDSTLTGKLC  60
   61 QTAITELRNE TGTSRYQFIA VRLPYGVQAD EADCQDAIAF IQPDRVLTVN IKPAIESSEA 120
  121 TLRAIGVELS DFVKGNEKAR ERMKAQYSIA GMNAGLVVGT DHAAEAVTGF FTKYGDGGTD 180
  181 INPIFRLNKR QGKALLRELG CPSHLYTKAP TADLEEDRPS LPDEVALGVT YEKIDDYLEG 240
  241 KPIDANDAAT IENWYRKTEH KRRPPITVFD DFWQ

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	71.30103
Match:	1wxeA
Description:	E.coli NAD Synthetase, AMP
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
ligase activity, forming carbon-nitrogen bonds	1.9422825865652	bayes_pls_golite062009
carbon-nitrogen ligase activity, with glutamine as amido-N-donor	1.77152913449838	bayes_pls_golite062009
GMP synthase (glutamine-hydrolyzing) activity	1.08094655947236	bayes_pls_golite062009
ligase activity	1.02544349488516	bayes_pls_golite062009
catalytic activity	0.828867994386867	bayes_pls_golite062009
NAD+ synthase activity	0.693146657234538	bayes_pls_golite062009
binding	0.268276895381766	bayes_pls_golite062009
protein binding	0.0198162482538781	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: