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Protein Overview: CstF-64-PA

General Information:
Name(s) found: CstF-64-PA / FBpp0083075 [FlyBase]
Description(s) found:
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  • gi|5713194|gb|AAD47839.1|AF170082_1 cleavage stimulation factor 64 kilodalton subunit [Drosophila melanogaster] [NCBI NR]
  • gi|23171661|gb|AAF55577.2| cleavage stimulation factor 64 kilodalton subunit [Drosophila melanogaster] [NCBI NR]
  • gi|205360993|gb|ACI03573.1| FI01908p [Drosophila melanogaster] [NCBI NR]
  • gi|17137710|ref|NP_477453.1| cleavage stimulation factor 64 kilodalton subunit [Drosophila melanogaster] [NCBI NR]
  • The gene Cleavage stimulation factor 64 kilodalton subunit is referred to in FlyBase by the symbol Dmel\CstF-64 (CG7697, FBgn0027841). It is a protein_coding_gene from Drosophila melanogaster. Its sequence location is 3R:14485114..14486850. It has the cytological map location 91B8. Based on sequence similarity, it is predicted to have molecular function</span>: mRNA binding. Based on sequence similarity, it is predicted to be involved in the biological process</span>: mRNA cleavage. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. [FlyBase]
  • >CG7697-PA translation from_gene[CG7697 CstF-64 FBgn0027841] seq_release:3 mol_weight=46186 cds_boundaries:(3R:14,485,444..14,486,763[+]) aa_length:419 transcript_info:[CG7697-RA seq_release:3] gene_info:[gene symbol:CstF-64 FBgn0027841 gene_boundaries [hmr_yeast5_DM3-1_pombe.fasta]
  • (Q9VE52) CG7697 protein (Cleavage stimulation factor 64 KILODALTON subunit) [DrosMelano7227.FASTAC.txt]
  • CG7697-PA [Drosophila melanogaster]gi|5713194|gb|AAD47839.1| cleavage stimulation factor 64 kilodalton subunit [Drosophila melanogaster]gi|17137710|ref|NP_477453.1| CG7697-PA [Drosophila melanogaster] [nrdb_11152004_Chlamydomonas]
  • type=protein; loc=3R:join(14485427..14485563,14485627..14486749); ID=FBpp0083075; name=CstF-64-PA; parent=FBgn0027841,FBtr0083660; dbxref=FlyBase_Annotation_IDs:CG7697-PA,FlyBase:FBpp0083075,GB_protein:AAF55577.2,REFSEQ:NP_477453,GB_protein:AAF55577; MD5= [fly-100309-contam.fasta]
  • Cleavage stimulation factor 64 kilodalton subunit CG7697-PA [Drosophila melanogaster] [nr-271106-contam.fasta]
  • CG7697-PA [Drosophila melanogaster] [nr-271106-contam.fasta]
  • cleavage stimulation factor 64 kilodalton subunit [Drosophila melanogaster] [nr-271106-contam.fasta]
  • type=protein; loc=3R:join(14485427..14485563,14485627..14486749); ID=FBpp0083075; name=CstF-64-PA; parent=FBgn0027841,FBtr0083660; dbxref=FlyBase_Annotation_IDs:CG7697-PA,FlyBase:FBpp0083075,GB_protein:AAF55577.2,REFSEQ:NP_477453,GB_protein:AAF55577; MD5=1a4d9437055297f002f91b19d2509127; length=419; release=r5.16; species=Dmel; [fly-contam-His-TEV-Tub4complex.fasta]
  • type=protein; loc=3R:join(14485427..14485563,14485627..14486749); ID=FBpp0083075; name=CstF-64-PA; parent=FBgn0027841,FBtr0083660; dbxref=FlyBase_Annotation_IDs:CG7697-PA,FlyBase:FBpp0083075,GB_protein:AAF55577.2,REFSEQ:NP_477453,GB_protein:AAF55577; MD5=1a4d9437055297f002f91b19d2509127; length=419; release=r5.21; species=Dmel; [Other_drosophila_na_09-09-2009_reversed.fasta]
  • Cleavage stimulation factor 64 kilodalton subunit OS=Drosophila melanogaster GN=CstF-64 PE=1 SV=2 [UniProt_drosophila_07-01-2011_reversed.fasta]
  • type=protein; loc=3R:join(14485427..14485563,14485627..14486749); ID=FBpp0083075; name=CstF-64-PA; parent=FBgn0027841,FBtr0083660; dbxref=FlyBase_Annotation_IDs:CG7697-PA,FlyBase:FBpp0083075,GB_protein:AAF55577.2,REFSEQ:NP_477453,GB_protein:AAF55577,FlyMine:FBpp0083075,modMine:FBpp0083075; MD5=1a4d9437055297f002f91b19d2509127; length=419; release=r5.46; species=Dmel; [fly-062812-sfps-sandra-contam.fasta]
  • Cleavage stimulation factor 64 kilodalton subunit OS=Drosophila melanogaster GN=CstF-64 PE=2 SV=2 [UniProt_D_Melanogaster_07132012_07-13-2012_reversed.fasta]
  • type=protein; loc=3R:join(18659705..18659841,18659905..18661027); ID=FBpp0083075; name=CstF-64-PA; parent=FBgn0027841,FBtr0083660; dbxref=FlyBase_Annotation_IDs:CG7697-PA,FlyBase:FBpp0083075,GB_protein:AAF55577.2,REFSEQ:NP_477453,GB_protein:AAF55577,FlyMine:FBpp0083075,modMine:FBpp0083075; MD5=1a4d9437055297f002f91b19d2509127; length=419; release=r6.02; species=Dmel; [fly-2014-05-contam.fasta]
  • type=protein; loc=3R:join(18659705..18659841,18659905..18661027); ID=FBpp0083075; name=CstF-64-PA; parent=FBgn0027841,FBtr0083660; dbxref=FlyBase_Annotation_IDs:CG7697-PA,FlyBase:FBpp0083075,GB_protein:AAF55577.2,REFSEQ:NP_477453,GB_protein:AAF55577,FlyMine:FBpp0083075,modMine:FBpp0083075; MD5=1a4d9437055297f002f91b19d2509127; length=419; release=r6.07; species=Dmel; [fly-08202015-contam.fasta]
  • FBpp0083075 type=protein; loc=3R:join(18659705..18659841,18659905..18661027); ID=FBpp0083075; name=CstF-64-PA; parent=FBgn0027841,FBtr0083660; dbxref=FlyBase_Annotation_IDs:CG7697-PA,FlyBase:FBpp0083075,GB_protein:AAF55577.2,REFSEQ:NP_477453,GB_protein:AAF55577,FlyMine:FBpp0083075,modMine:FBpp0083075; MD5=1a4d9437055297f002f91b19d2509127; length=419; release=r6.09; species=Dmel; [dmel-all-translation-r6.09.fasta]
  • [fly-122215-dmel-6-09-contam.fasta]
  • type=protein; loc=3R:join(18659705..18659841,18659905..18661027); ID=FBpp0083075; name=CstF-64-PA; parent=FBgn0027841,FBtr0083660; dbxref=FlyBase_Annotation_IDs:CG7697-PA,FlyBase:FBpp0083075,GB_protein:AAF55577.2,REFSEQ:NP_477453,GB_protein:AAF55577,UniProt/TrEMBL:Q9VE52,FlyMine:FBpp0083075,modMine:FBpp0083075; MD5=1a4d9437055297f002f91b19d2509127; length=419; release=r6.14; species=Dmel; [fly-dmel-jan2017-sandracontam.fasta]
    • Organism: Drosophila melanogaster
    Length: 419 amino acids

    Gene Ontology:
    Cellular Component: mRNA cleavage stimulating factor complex [ISS][NAS]
    Biological Process: mRNA cleavage [ISS][NAS]
    Molecular Function: nucleotide binding [IEA]
    nucleic acid binding [IEA]
    mRNA binding [ISS]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MADKAQEQSI MDKSMRSVFV GNIPYEATEE KLKEIFSEVG PVLSLKLVFD RESGKPKGFG  60
   61 FCEYKDQETA LSAMRNLNGY EIGGRTLRVD NACTEKSRME MQQLLQGPQV ENPYGEPCEP 120
  121 EDAPELITKT VASLPPEQMY ELMKQMKLCI VSNPSEARQM LMLNPQLAYA LLQAMVVMRI 180
  181 VDPQQALGML FKANQMPPVL GGNPHQGPGN HTMMGQQQVP QQQVQIPQQQ QQAPQPPMPV 240
  241 PGPGFPANVH PNDIDLRMVP GGPMPMDPRM MGRGMDQDLR ASLPNPVPPP LMDPRARAQM 300
  301 PPQQQQGVPQ APPAPYPSDP RQRPMDPRLR AGPGPQQQAP PQGIPQAPPP TQQQQAAAQQ 360
  361 LQSRLGAHGV LPSDASDQEK AALIMQVLQL SDEQIAQLPS EQRVSIVMLK EQIAKSTQR

    Protein Complex Data

    No complex found for this protein.

    Mass Spectrometry Data

    No mass spectrometry results found for this protein.

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..189] PSI-BLAST 22.522879 NMR Structure of the N-terminal RRM domain of Cleavage stimulation factor 64 KDa subunit
    2 View Details [190..263] deduced N/A No confident structure predictions are available.
    3 View Details [264..419] PSI-BLAST 8.0 Sec24

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • RNA binding
    		•  5.15097136329571 bayes_pls_golite062009
  • mRNA binding
    		•  4.05281507540116 bayes_pls_golite062009
  • nucleic acid binding
    		•  3.81920018903278 bayes_pls_golite062009
    		• 3.4479220904723 bayes_pls_golite062009
  • binding
    		•  3.05116833253954 bayes_pls_golite062009
  • single-stranded RNA binding
    		•  1.46469774223879 bayes_pls_golite062009
  • poly-pyrimidine tract binding
    		•  1.36691748240059 bayes_pls_golite062009
  • protein binding
    		•  1.14508556628804 bayes_pls_golite062009
  • transcription regulator activity
    		•  1.07788694919471 bayes_pls_golite062009
  • DNA binding
    		•  0.978604858165557 bayes_pls_golite062009
  • snRNA binding
    		•  0.73428616955527 bayes_pls_golite062009
  • mRNA 3'-UTR binding
    		•  0.591700155096213 bayes_pls_golite062009
  • transcription factor activity
    		•  0.234742017105535 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.


    Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.97

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle