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Protein Overview: BRF1

General Information:
Name(s) found: BRF1 / YGR246C [SGD]
Description(s) found:
Found 32 descriptions.
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  • TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 596 amino acids

    Gene Ontology:
    Cellular Component: transcription factor TFIIIB complex [TASLink to PubMed article]
    Biological Process: transcription initiation from RNA polymerase III promoter [TASLink to PubMed article]
    Molecular Function: RNA polymerase III transcription factor activity [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPVCKNCHGT EFERDLSNAN NDLVCKACGV VSEDNPIVSE VTFGETSAGA AVVQGSFIGA  60
   61 GQSHAAFGGS SALESREATL NNARRKLRAV SYALHIPEYI TDAAFQWYKL ALANNFVQGR 120
  121 RSQNVIASCL YVACRKEKTH HMLIDFSSRL QVSVYSIGAT FLKMVKKLHI TELPLADPSL 180
  181 FIQHFAEKLD LADKKIKVVK DAVKLAQRMS KDWMFEGRRP AGIAGACILL ACRMNNLRRT 240
  241 HTEIVAVSHV AEETLQQRLN EFKNTKAAKL SVQKFRENDV EDGEARPPSF VKNRKKERKI 300
  301 KDSLDKEEMF QTSEEALNKN PILTQVLGEQ ELSSKEVLFY LKQFSERRAR VVERIKATNG 360
  361 IDGENIYHEG SENETRKRKL SEVSIQNEHV EGEDKETEGT EEKVKKVKTK TSEEKKENES 420
  421 GHFQDAIDGY SLETDPYCPR NLHLLPTTDT YLSKVSDDPD NLEDVDDEEL NAHLLNEEAS 480
  481 KLKERIWIGL NADFLLEQES KRLKQEADIA TGNTSVKKKR TRRRNNTRSD EPTKTVDAAA 540
  541 AIGLMSDLQD KSGLHAALKA AEESGDFTTA DSVKNMLQKA SFSKKINYDA IDGLFR

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details (MIPS) Mewes HW, et al. (2004) BDP1 BRF1 SPT15
    View Details Krogan NJ, et al. (2006) BRF1 BRL1 SPT15
    View Details Qiu et al. (2008) BDP1 BRF1 SPT15 TFB5 TFC1 TFC4 TFC6 TFC8

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run None Entered #17 Mitotic Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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      PROTEIN(S) PUBLICATION
    View Data BRF1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..61] PSI-BLAST 6.69897 Transcription inititiation factor TFIIB, N-terminal domain
    2 View Details [62..175] PSI-BLAST 189.154902 Transcription factor IIB (TFIIB), core domain
    3 View Details [176..281] PSI-BLAST 189.154902 Transcription factor IIB (TFIIB), core domain
    4 View Details [282..596] MSA 3.294996 View MSA. No confident structure predictions are available.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.95

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle