YRC Public Data Repository - Protein Structure Prediction

Protein:	YBDK_ECOLI, CAAL...
Organism:	Escherichia coli
Length:	372 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for YBDK_ECOLI, CAAL....

Description	E-value	Query Range	Subject Range
GCS2_BURXL - Putative glutamate--cysteine ligase 2 OS=Burkholderia xenovorans (strain LB400) GN=Bxeno_A4387 PE=3 ...	366.0	[0..1]	[371..1]
gi\|84363383 - gi\|84363383\|ref\|ZP_00987971.1\| COG2170: Uncharacterized conserved protein [Burkholderia dolosa AUO15...	363.0	[0..1]	[371..1]
GCS2_BURA4 - Putative glutamate--cysteine ligase 2 OS=Burkholderia ambifaria (strain MC40-6) GN=BamMC406_0068 PE=... GCS2_BURCM - Putative glutamate--cysteine ligase 2 OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) GN=Bamb... gi\|171095974, gi... - gi\|171318833\|ref\|ZP_02907970.1\| glutamate--cysteine ligase GCS2 [Burkholderia ambifaria MEX-5], gi\|1... gi\|170136067, gi... - gi\|170699031\|ref\|ZP_02890088.1\| glutamate--cysteine ligase GCS2 [Burkholderia ambifaria IOP40-10], g...	361.0	[0..1]	[371..1]
GCS2_BURCC - Putative glutamate--cysteine ligase 2 OS=Burkholderia cenocepacia (strain MC0-3) GN=Bcenmc03_0087 PE... GCS2_BURCJ - Putative glutamate--cysteine ligase 2 OS=Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 /... GCS2_BURCA - Putative glutamate--cysteine ligase 2 OS=Burkholderia cenocepacia (strain AU 1054) GN=Bcen_0001 PE=3... gi\|84356372 - gi\|84356372\|ref\|ZP_00981220.1\| COG2170: Uncharacterized conserved protein [Burkholderia cenocepacia ... GCS2_BURCH - Putative glutamate--cysteine ligase 2 OS=Burkholderia cenocepacia (strain HI2424) GN=Bcen2424_0069 P...	359.0	[0..1]	[371..1]
gi\|167921112 - gi\|167921112\|ref\|ZP_02508203.1\| carboxylate-amine ligase [Burkholderia pseudomallei BCC215] gi\|254174738, gi... - gi\|254174738\|ref\|ZP_04881399.1\| carboxylate-amine ligase family protein [Burkholderia mallei ATCC 10... gi\|167904894 - gi\|167904894\|ref\|ZP_02492099.1\| carboxylate-amine ligase [Burkholderia pseudomallei NCTC 13177] gi\|167913174 - gi\|167913174\|ref\|ZP_02500265.1\| carboxylate-amine ligase [Burkholderia pseudomallei 112] gi\|217424014, gi... - gi\|217424014\|ref\|ZP_03455514.1\| carboxylate-amine ligase family protein [Burkholderia pseudomallei 5... gi\|167721871 - gi\|167721871\|ref\|ZP_02405107.1\| carboxylate-amine ligase [Burkholderia pseudomallei DM98] gi\|167740840 - gi\|167740840\|ref\|ZP_02413614.1\| carboxylate-amine ligase [Burkholderia pseudomallei 14] gi\|167001297, gi... - gi\|243062914\|gb\|EES45100.1\| carboxylate-amine ligase family protein [Burkholderia mallei PRL-20], gi... gi\|167847936 - gi\|167847936\|ref\|ZP_02473444.1\| carboxylate-amine ligase [Burkholderia pseudomallei B7210] gi\|167896494 - gi\|167896494\|ref\|ZP_02483896.1\| carboxylate-amine ligase [Burkholderia pseudomallei 7894] gi\|167818055 - gi\|167818055\|ref\|ZP_02449735.1\| carboxylate-amine ligase [Burkholderia pseudomallei 91] gi\|167826420 - gi\|167826420\|ref\|ZP_02457891.1\| carboxylate-amine ligase [Burkholderia pseudomallei 9] GCS2_BURP1 - Putative glutamate--cysteine ligase 2 OS=Burkholderia pseudomallei (strain 1710b) GN=BURPS1710b_0214... GCS2_BURPS - Putative glutamate--cysteine ligase 2 OS=Burkholderia pseudomallei (strain K96243) GN=BPSL0001 PE=3 ... GCS2_BURM9 - Putative glutamate--cysteine ligase 2 OS=Burkholderia mallei (strain NCTC 10229) GN=BMA10229_A1626 P... GCS2_BURMA - Putative glutamate--cysteine ligase 2 OS=Burkholderia mallei (strain ATCC 23344) GN=BMA2917 PE=3 SV=... CAAL_BURMA - Carboxylate-amine ligase BMA2917 OS=Burkholderia mallei GN=BMA2917 PE=3 SV=1 gi\|85065956 - gi\|85065956\|ref\|ZP_01026809.1\| COG2170: Uncharacterized conserved protein [Burkholderia mallei 10229... gi\|148026455, gi... - gi\|87316705\|ref\|ZP_01097719.1\| COG2170: Uncharacterized conserved protein [Burkholderia mallei 20027... gi\|157934215, gi... - gi\|67755079\|ref\|ZP_00493978.1\| COG2170: Uncharacterized conserved protein [Burkholderia pseudomallei... gi\|100122536, gi... - gi\|67760093\|ref\|ZP_00498819.1\| COG2170: Uncharacterized conserved protein [Burkholderia pseudomallei... CAAL_BURPS - Carboxylate-amine ligase BPSL0001 OS=Burkholderia pseudomallei GN=BPSL0001 PE=3 SV=1 gi\|238524300, gi... - gi\|67643056\|ref\|ZP_00441805.1\| carboxylate-amine ligase YbdK [Burkholderia mallei GB8 horse 4], gi\|2... gi\|226193306, gi... - gi\|226193306\|ref\|ZP_03788916.1\| carboxylate-amine ligase family protein [Burkholderia pseudomallei P... GCS2_BURM7 - Putative glutamate--cysteine ligase 2 OS=Burkholderia mallei (strain NCTC 10247) GN=BMA10247_2975 PE... gi\|67637400, gi\|... - gi\|67637400\|ref\|ZP_00436340.1\| COG2170: Uncharacterized conserved protein [Burkholderia mallei 10399... gi\|254201498, gi... - gi\|83621194\|ref\|ZP_00931605.1\| COG2170: Uncharacterized conserved protein [Burkholderia mallei FMH],... gi\|237814391, gi... - gi\|237814391\|ref\|YP_002898842.1\| carboxylate-amine ligase _2975 [Burkholderia pseudomallei MSHR346],... GCS2_BURP6 - Putative glutamate--cysteine ligase 2 OS=Burkholderia pseudomallei (strain 668) GN=BURPS668_0001 PE=... GCS2_BURMS - Putative glutamate--cysteine ligase 2 OS=Burkholderia mallei (strain SAVP1) GN=BMASAVP1_A3394 PE=3 S... gi\|67673934, gi\|... - gi\|99913426\|ref\|ZP_01320075.1\| hypothetical protein Bpse1_03000594 [Burkholderia pseudomallei 1655],... gi\|67681962, gi\|... - gi\|90291181\|ref\|ZP_01210814.1\| hypothetical protein Bpse17_02004166 [Burkholderia pseudomallei 1710a... gi\|254206837, gi... - gi\|83624016\|ref\|ZP_00934272.1\| COG2170: Uncharacterized conserved protein [Burkholderia mallei JHU],... gi\|157808592, gi... - gi\|83679122\|ref\|ZP_00940839.1\| COG2170: Uncharacterized conserved protein [Burkholderia pseudomallei... GCS2_BURP0 - Putative glutamate--cysteine ligase 2 OS=Burkholderia pseudomallei (strain 1106a) GN=BURPS1106A_0001... gi\|161359638, gi... - gi\|90662094\|ref\|ZP_01249930.1\| hypothetical protein Bpse110_02006071 [Burkholderia pseudomallei 1106... gi\|134281344, gi... - gi\|134281344\|ref\|ZP_01768052.1\| carboxylate-amine ligase family protein [Burkholderia pseudomallei 3...	359.0	[0..1]	[371..1]
GCS2_CUPMC - Putative glutamate--cysteine ligase 2 OS=Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / N... gi\|224083422, gi... - gi\|224083422\|ref\|XP_002335402.1\| predicted protein [Populus trichocarpa], gi\|222833742\|gb\|EEE72219.1...	359.0	[0..1]	[369..1]
CAAL_BURS3 - Carboxylate-amine ligase Bcep18194_A3250 OS=Burkholderia sp. (strain 383) GN=Bcep18194_A3250 PE=3 SV... GCS2_BURL3 - Putative glutamate--cysteine ligase 2 OS=Burkholderia lata (strain ATCC 17760 / LMG 22485 / NCIMB 90...	358.0	[0..1]	[371..1]
gi\|191169320, gi... - gi\|75227368\|ref\|ZP_00714146.1\| COG2170: Uncharacterized conserved protein [Escherichia coli B7A], gi... GCS2_ECO24 - Putative glutamate--cysteine ligase 2 OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=ybdK P...	357.0	[0..1]	[372..1]
gi\|83586985, gi\|... - gi\|83586985\|ref\|ZP_00925615.1\| COG2170: Uncharacterized conserved protein [Escherichia coli 101-1], ...	356.0	[0..1]	[372..1]
GCS2_SHIBS - Putative glutamate--cysteine ligase 2 OS=Shigella boydii serotype 4 (strain Sb227) GN=ybdK PE=3 SV=1	356.0	[0..1]	[372..1]

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Predicted Domain #1

Region A:
Residues: [1-372]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPLPDFHVSE PFTLGIELEM QVVNPPGYDL SQDSSMLIDA VKNKITAGEV KHDITESMLE  60
   61 LATDVCRDIN QAAGQFSAMQ KVVLQAATDH HLEICGGGTH PFQKWQRQEV CDNERYQRTL 120
  121 ENFGYLIQQA TVFGQHVHVG CASGDDAIYL LHGLSRFVPH FIALSAASPY MQGTDTRFAS 180
  181 SRPNIFSAFP DNGPMPWVSN WQQFEALFRC LSYTTMIDSI KDLHWDIRPS PHFGTVEVRV 240
  241 MDTPLTLSHA VNMAGLIQAT AHWLLTERPF KHQEKDYLLY KFNRFQACRY GLEGVITDPH 300
  301 TGDRRPLTED TLRLLEKIAP SAHKIGASSA IEALHRQVVS GLNEAQLMRD FVADGGSLIG 360
  361 LVKKHCEIWA GD

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	98.0
Match:	1r8gA
Description:	Structure and function of YbdK
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
glutamate-cysteine ligase activity	3.038917403359	bayes_pls_golite062009
catalytic activity	1.46017961385003	bayes_pls_golite062009
ligase activity	0.321261148816799	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	0.261435572945741	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: