YRC Public Data Repository - Protein Structure Prediction

Protein:	T1MK_ECOLI
Organism:	Escherichia coli
Length:	529 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for T1MK_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|242379864, gi... - gi\|251787591\|ref\|YP_003001895.1\| hypothetical protein B21_04181 [Escherichia coli BL21], gi\|24237986... gi\|253775030, gi... - gi\|254291017\|ref\|YP_003056767.1\| DNA methylase M [Escherichia coli BL21(DE3)], gi\|253980326\|gb\|ACT45... gi\|146398 - gi\|146398\|gb\|AAA23985.1\| restriction-modification enzyme type I M subunit [Escherichia coli]	401.0	[0..1]	[529..1]
gi\|115515785, gi... - gi\|117626661\|ref\|YP_859984.1\| DNA methylase M [Escherichia coli APEC O1], gi\|115515785\|gb\|ABJ03860.1... gi\|91075573, gi\|... - gi\|91213999\|ref\|YP_543985.1\| DNA methylase M [Escherichia coli UTI89], gi\|91075573\|gb\|ABE10454.1\| DN...	396.0	[0..1]	[529..1]
gi\|154132 - gi\|154132\|gb\|AAA27143.1\| restriction-modification enzyme type I M subunit T1M_SALPO - Type I restriction enzyme StySPI M protein OS=Salmonella potsdam GN=hsdM PE=3 SV=2	396.0	[0..1]	[528..1]
T1M_SALTY - Type I restriction enzyme StySJI M protein OS=Salmonella typhimurium GN=hsdM PE=3 SV=1 T1M_SALTY - Type I restriction enzyme StySJI M protein OS=Salmonella typhimurium GN=hsdM PE=3 SV=1 gi\|197262701, gi... - gi\|197262701\|ref\|ZP_03162775.1\| type I restriction enzyme EcoKI M protein [Salmonella enterica subsp... T1M_SALTY - Type I restriction enzyme StySJI M protein OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 7... gi\|154130 - gi\|154130\|gb\|AAA19429.1\| restriction-modification enzyme type I M subunit [Salmonella enterica]	388.0	[0..1]	[528..1]
gi\|197365232, gi... - gi\|197365232\|ref\|YP_002144869.1\| DNA methylase M, host modification [Salmonella enterica subsp. ente... gi\|56416309, gi\|... - gi\|56416309\|ref\|YP_153384.1\| DNA methylase M, host modification [Salmonella enterica subsp. enterica...	382.0	[0..1]	[529..1]
gi\|90329500, gi\|... - gi\|90410147\|ref\|ZP_01218164.1\| DNA methylase M, host modification [Photobacterium profundum 3TCK], g...	376.0	[0..1]	[529..1]
gi\|194536908, gi... - gi\|75818015\|ref\|ZP_00748248.1\| COG0286: Type I restriction-modification system methyltransferase sub...	357.0	[0..1]	[525..1]
gi\|77954342, gi\|... - gi\|77954342\|ref\|ZP_00818738.1\| type I restriction-modification system, M subunit [Marinobacter aquae...	346.0	[0..1]	[528..1]
gi\|84390142, gi\|... - gi\|84390142\|ref\|ZP_00991404.1\| type I restriction-modification system, M subunit [Vibrio splendidus ...	344.0	[0..1]	[529..1]

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Predicted Domain #1

Region A:
Residues: [1-529]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNNNDLVAKL WKLCDNLRDG GVSYQNYVNE LASLLFLKMC KETGQEAEYL PEGYRWDDLK  60
   61 SRIGQEQLQF YRKMLVHLGE DDKKLVQAVF HNVSTTITEP KQITALVSNM DSLDWYNGAH 120
  121 GKSRDDFGDM YEGLLQKNAN ETKSGAGQYF TPRPLIKTII HLLKPQPREV VQDPAAGTAG 180
  181 FLIEADRYVK SQTNDLDDLD GDTQDFQIHR AFIGLELVPG TRRLALMNCL LHDIEGNLDH 240
  241 GGAIRLGNTL GSDGENLPKA HIVATNPPFG SAAGTNITRT FVHPTSNKQL CFMQHIIETL 300
  301 HPGGRAAVVV PDNVLFEGGK GTDIRRDLMD KCHLHTILRL PTGIFYAQGV KTNVLFFTKG 360
  361 TVANPNQDKN CTDDVWVYDL RTNMPSFGKR TPFTDEHLQP FERVYGEDPH GLSPRTEGEW 420
  421 SFNAEETEVA DSEENKNTDQ HLATSRWRKF SREWIRTAKS DSLDISWLKD KDSIDADSLP 480
  481 EPDVLAAEAM GELVQALSEL DALMRELGAS DEADLQRQLL EEAFGGVKE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	110.0
Match:	2ar0A
Description:	Crystal structure of Type I restriction enzyme EcoKI M protein (EC 2.1.1.72) (M.EcoKI)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
DNA-methyltransferase activity	7.61853880993981	bayes_pls_golite062009
site-specific DNA-methyltransferase (adenine-specific) activity	7.11249059771057	bayes_pls_golite062009
S-adenosylmethionine-dependent methyltransferase activity	4.4632173842967	bayes_pls_golite062009
methyltransferase activity	4.15445027778357	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	4.07153733729662	bayes_pls_golite062009
N-methyltransferase activity	3.39676833658685	bayes_pls_golite062009
protein methyltransferase activity	3.33061049943022	bayes_pls_golite062009
histone methyltransferase activity	2.22830304942698	bayes_pls_golite062009
transferase activity	2.22303238506686	bayes_pls_golite062009
protein-arginine N-methyltransferase activity	1.89831891552024	bayes_pls_golite062009
arginine N-methyltransferase activity	1.89831891552024	bayes_pls_golite062009
histone-arginine N-methyltransferase activity	1.30685781453463	bayes_pls_golite062009
catalytic activity	1.25333584501255	bayes_pls_golite062009
protein-arginine omega-N symmetric methyltransferase activity	1.00318794562816	bayes_pls_golite062009
protein-arginine omega-N monomethyltransferase activity	0.950437293690352	bayes_pls_golite062009
DNA (cytosine-5-)-methyltransferase activity	0.937171599492037	bayes_pls_golite062009
binding	0.907448038004683	bayes_pls_golite062009
RNA methyltransferase activity	0.817509452491588	bayes_pls_golite062009
transcription regulator activity	0.517021612434243	bayes_pls_golite062009
DNA binding	0.508133107367239	bayes_pls_golite062009
nucleic acid binding	0.443691572120283	bayes_pls_golite062009
histone methyltransferase activity (H4-R3 specific)	0.421039991390153	bayes_pls_golite062009
protein-arginine omega-N asymmetric methyltransferase activity	0.40782425952463	bayes_pls_golite062009
rRNA methyltransferase activity	0.230719796976667	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: