YRC Public Data Repository - Protein Structure Prediction

Protein:	HYFG_ECOLI
Organism:	Escherichia coli
Length:	555 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for HYFG_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|157067627, gi... - gi\|75196495\|ref\|ZP_00706565.1\| COG3261: Ni,Fe-hydrogenase III large subunit [Escherichia coli HS], g...	545.0	[0..1]	[555..1]
gi\|194414699, gi... - gi\|75211454\|ref\|ZP_00711544.1\| COG3261: Ni,Fe-hydrogenase III large subunit [Escherichia coli B171],...	544.0	[0..1]	[555..1]
gi\|75257294, gi\|... - gi\|75257294\|ref\|ZP_00728818.1\| COG3261: Ni,Fe-hydrogenase III large subunit [Escherichia coli E22], ...	544.0	[0..1]	[555..1]
gi\|254794432, gi... - gi\|254794432\|ref\|YP_003079269.1\| hydrogenase 4, subunit [Escherichia coli O157:H7 str. TW14359], gi\|... gi\|217319961, gi... - gi\|217327594\|ref\|ZP_03443677.1\| hydrogenase-4, G subunit [Escherichia coli O157:H7 str. TW14588], gi... gi\|209161380, gi... - gi\|209399980\|ref\|YP_002271956.1\| hydrogenase-4, G subunit [Escherichia coli O157:H7 str. EC4115], gi... gi\|208819807, gi... - gi\|208819807\|ref\|ZP_03260127.1\| hydrogenase-4, G subunit [Escherichia coli O157:H7 str. EC4042], gi\|... gi\|208814570, gi... - gi\|208814570\|ref\|ZP_03255899.1\| hydrogenase-4, G subunit [Escherichia coli O157:H7 str. EC4045], gi\|... gi\|15832603, gi\|... - gi\|15832603\|ref\|NP_311376.1\| hydrogenase 4 subunit [Escherichia coli O157:H7 str. Sakai], gi\|1336281... gi\|168778450, gi... - gi\|189003251\|gb\|EDU72237.1\| hydrogenase-4, G subunit [Escherichia coli O157:H7 str. EC4076], gi\|1687... gi\|168773602, gi... - gi\|187770667\|gb\|EDU34511.1\| hydrogenase-4, G subunit [Escherichia coli O157:H7 str. EC4196], gi\|1687... gi\|168768576, gi... - gi\|189362326\|gb\|EDU80745.1\| hydrogenase-4, G subunit [Escherichia coli O157:H7 str. EC4486], gi\|1687... gi\|189368454, gi... - gi\|189368454\|gb\|EDU86870.1\| hydrogenase-4, G subunit [Escherichia coli O157:H7 str. EC4501], gi\|1687... gi\|208726483, gi... - gi\|208806682\|ref\|ZP_03249019.1\| hydrogenase-4, G subunit [Escherichia coli O157:H7 str. EC4206], gi\|... gi\|195936630 - gi\|195936630\|ref\|ZP_03082012.1\| hydrogenase 4 subunit [Escherichia coli O157:H7 str. EC4024] gi\|189378645, gi... - gi\|189378645\|gb\|EDU97061.1\| hydrogenase-4, G subunit [Escherichia coli O157:H7 str. EC508], gi\|16879... gi\|168787830, gi... - gi\|189372352\|gb\|EDU90768.1\| hydrogenase-4, G subunit [Escherichia coli O157:H7 str. EC869], gi\|16878... gi\|25285220, gi\|... - pir\|\|A85892 hydrogenase 4 subunit [imported] - Escherichia coli (strain O157:H7, substrain EDL933),... gi\|12516872, gi\|... - gi\|15803010\|ref\|NP_289040.1\| hydrogenase 4 subunit [Escherichia coli O157:H7 EDL933], gi\|12516872\|gb... gi\|15832603, gi\|... - gi\|15832603\|ref\|NP_311376.1\| hydrogenase 4 subunit [Escherichia coli O157:H7], gi\|13362819\|dbj\|BAB36... gi\|189356600, gi... - gi\|189356600\|gb\|EDU75019.1\| hydrogenase-4, G subunit [Escherichia coli O157:H7 str. EC4401], gi\|1687... gi\|168748457, gi... - gi\|188017010\|gb\|EDU55132.1\| hydrogenase-4, G subunit [Escherichia coli O157:H7 str. EC4113], gi\|1687...	539.0	[0..1]	[555..1]
gi\|82777868, gi\|... - gi\|82777868\|ref\|YP_404217.1\| hydrogenase 4 subunit [Shigella dysenteriae Sd197], gi\|81242016\|gb\|ABB6...	537.0	[0..1]	[555..1]
gi\|73856490, gi\|... - gi\|74313013\|ref\|YP_311432.1\| hydrogenase 4 subunit [Shigella sonnei Ss046], gi\|73856490\|gb\|AAZ89197....	536.0	[0..1]	[555..1]
gi\|194419705, gi... - gi\|83570704\|ref\|ZP_00922147.1\| COG3261: Ni,Fe-hydrogenase III large subunit [Shigella dysenteriae 10...	536.0	[0..1]	[555..1]
gi\|75237766, gi\|... - gi\|75237766\|ref\|ZP_00721782.1\| COG3261: Ni,Fe-hydrogenase III large subunit [Escherichia coli E11001... gi\|157079110, gi... - gi\|75187238\|ref\|ZP_00700505.1\| COG3261: Ni,Fe-hydrogenase III large subunit [Escherichia coli E24377...	535.0	[0..1]	[555..1]
gi\|83591009, gi\|... - gi\|83591009\|ref\|YP_431018.1\| NADH dehydrogenase (ubiquinone), 30 kDa subunit [Moorella thermoacetica...	535.0	[0..1]	[555..1]
gi\|81246347, gi\|... - gi\|82544936\|ref\|YP_408883.1\| hydrogenase 4 subunit [Shigella boydii Sb227], gi\|81246347\|gb\|ABB67055....	535.0	[0..1]	[555..1]

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Predicted Domain #1

Region A:
Residues: [1-170]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNVNSSSNRG EAILAALKTQ FPGAVLDEER QTPEQVTITV KINLLPDVVQ YLYYQHDGWL  60
   61 PVLFGNDERT LNGHYAVYYA LSMEGAEKCW IVVKALVDAD SREFPSVTPR VPAAVWGERE 120
  121 IRDMYGLIPV GLPDQRRLVL PDDWPEDMHP LRKDAMDYRL RPEPTTDSET

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	25.69897
Match:	2fug5
Description:	Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus
Matching Structure (courtesy of the PDB):

Predicted Domain #2

Region A:
Residues: [171-555]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 YPFINEGNSD ARVIPVGPLH ITSDEPGHFR LFVDGEQIVD ADYRLFYVHR GMEKLAETRM  60
   61 GYNEVTFLSD RVCGICGFAH SVAYTNSVEN ALGIEVPQRA HTIRSILLEV ERLHSHLLNL 120
  121 GLSCHFVGFD TGFMQFFRVR EKSMTMAELL IGSRKTYGLN LIGGVRRDIL KEQRLQTLKL 180
  181 VREMRADVSE LVEMLLATPN MEQRTQGIGI LDRQIARDLR FDHPYADYGN IPKTLFTFTG 240
  241 GDVFSRVMVR VKETFDSLAM LEFALDNMPD TPLLTEGFSY KPHAFALGFV EAPRGEDVHW 300
  301 SMLGDNQKLF RWRCRAATYA NWPVLRYMLR GNTVSDAPLI IGSLDPCYSC TDRVTLVDVR 360
  361 KRQSKTVPYK EIERYGIDRN RSPLK

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	98.0
Match:	2fug4
Description:	Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
NADH dehydrogenase activity	13.5914597754866	bayes_pls_golite062009
NADH dehydrogenase (quinone) activity	12.311192040385	bayes_pls_golite062009
NADH dehydrogenase (ubiquinone) activity	12.1563727867842	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor	11.7527486559512	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH	11.1653559876285	bayes_pls_golite062009
oxidoreductase activity	6.49653654839585	bayes_pls_golite062009
ferredoxin hydrogenase activity	3.63852106070173	bayes_pls_golite062009
oxidoreductase activity, acting on hydrogen as donor, iron-sulfur protein as acceptor	3.63852106070173	bayes_pls_golite062009
oxidoreductase activity, acting on hydrogen as donor	3.45611813853803	bayes_pls_golite062009
oxidoreduction-driven active transmembrane transporter activity	2.72460560332491	bayes_pls_golite062009
oxidoreductase activity, acting on iron-sulfur proteins as donors	2.45015987642707	bayes_pls_golite062009
electron carrier activity	1.58392879810571	bayes_pls_golite062009
catalytic activity	1.13542026154948	bayes_pls_golite062009
binding	0.73814915099044	bayes_pls_golite062009
protein binding	0.060184989967058	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: