YRC Public Data Repository - Protein Structure Prediction

Protein:	BIRA_ECOLI
Organism:	Escherichia coli
Length:	321 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for BIRA_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|84377441, gi\|... - gi\|84387758\|ref\|ZP_00990774.1\| BirA bifunctional protein [Vibrio splendidus 12B01], gi\|84377441\|gb\|E...	300.0	[0..1]	[320..1]
gi\|224585685, gi... - gi\|224585685\|ref\|YP_002639484.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar... gi\|62130229, gi\|... - gi\|62182596\|ref\|YP_219013.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar Cho...	299.0	[0..1]	[320..1]
gi\|104744493 - gi\|104744493\|gb\|ABF74577.1\| biotin ligase [Mammalian expression vector pCBio] gi\|104744458 - gi\|104744458\|gb\|ABF74574.1\| biotin ligase [Mammalian expression vector pNBio]	298.0	[0..1]	[321..14]
gi\|213580233 - gi\|213580233\|ref\|ZP_03362059.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar ... gi\|213428735 - gi\|213428735\|ref\|ZP_03361485.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar ... gi\|213163737 - gi\|213163737\|ref\|ZP_03349447.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar ... gi\|207859322, gi... - gi\|207859322\|ref\|YP_002245973.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar... gi\|213999716 - gi\|213999716\|ref\|ZP_03410451.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar ... gi\|213854978 - gi\|213854978\|ref\|ZP_03383218.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar ... gi\|213649752 - gi\|213649752\|ref\|ZP_03379805.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar ... gi\|213612440 - gi\|213612440\|ref\|ZP_03370266.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar ... gi\|29139433, gi\|... - gi\|29143798\|ref\|NP_807140.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar Typ... gi\|238913976 - gi\|238913976\|ref\|ZP_04657813.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar ... gi\|16762310 - gi\|16762310\|ref\|NP_457927.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar Typ... gi\|25293808, gi\|... - gi\|25293808\|pir\|\|AG0934 bifunctional protein, biotin operon repressor and biotin-[acetyl-CoA carboxy... gi\|56415970, gi\|... - gi\|56415970\|ref\|YP_153045.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar Par... gi\|194734870, gi... - gi\|194734870\|ref\|YP_002117046.1\| biotin-[acetyl-CoA-carboxylase] ligase [Salmonella enterica subsp. ... gi\|194457909, gi... - gi\|194471545\|ref\|ZP_03077529.1\| biotin-[acetyl-CoA-carboxylase] ligase [Salmonella enterica subsp. e... gi\|194449709, gi... - gi\|194449709\|ref\|YP_002048130.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar... gi\|194402221, gi... - gi\|194443558\|ref\|YP_002043395.1\| biotin-[acetyl-CoA-carboxylase] ligase [Salmonella enterica subsp. ... gi\|205354453, gi... - gi\|205354453\|ref\|YP_002228254.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar... gi\|198245209, gi... - gi\|198245209\|ref\|YP_002218061.1\| biotin-[acetyl-CoA-carboxylase] ligase [Salmonella enterica subsp. ... gi\|197096374, gi... - gi\|197364897\|ref\|YP_002144534.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar... gi\|197251475, gi... - gi\|197251475\|ref\|YP_002149054.1\| biotin-[acetyl-CoA-carboxylase] ligase [Salmonella enterica subsp. ... gi\|205331585, gi... - gi\|205331585\|gb\|EDZ18349.1\| biotin-[acetyl-CoA-carboxylase] ligase [Salmonella enterica subsp. enter... gi\|205321454, gi... - gi\|205321454\|gb\|EDZ09293.1\| biotin-[acetyl-CoA-carboxylase] ligase [Salmonella enterica subsp. enter... gi\|161617278, gi... - gi\|161617278\|ref\|YP_001591243.1\| biotin--protein ligase [Salmonella enterica subsp. enterica serovar... gi\|168823315, gi... - gi\|205340454\|gb\|EDZ27218.1\| biotin-[acetyl-CoA-carboxylase] ligase [Salmonella enterica subsp. enter... gi\|168262954, gi... - gi\|205348231\|gb\|EDZ34862.1\| biotin-[acetyl-CoA-carboxylase] ligase [Salmonella enterica subsp. enter... gi\|168245297, gi... - gi\|205335978\|gb\|EDZ22742.1\| biotin-[acetyl-CoA-carboxylase] ligase [Salmonella enterica subsp. enter... gi\|197287551, gi... - gi\|197287551\|gb\|EDY26944.1\| biotin-[acetyl-CoA-carboxylase] ligase [Salmonella enterica subsp. enter...	298.0	[0..1]	[320..1]
lcl\|NC_016856.1_... - [gene=birA] [protein=biotin--protein ligase] [protein_id=YP_005240421.1] [location=4371278..4372240] BIRA_SALTY - Bifunctional ligase/repressor BirA OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) G... BIRA_SALTY - Bifunctional protein BirA OS=Salmonella typhimurium GN=birA PE=3 SV=2 BIRA_SALTY - Bifunctional protein birA OS=Salmonella typhimurium GN=birA PE=3 SV=2 gi\|205326992, gi... - gi\|205326992\|gb\|EDZ13756.1\| biotin-[acetyl-CoA-carboxylase] ligase [Salmonella enterica subsp. enter... gi\|197239850, gi... - gi\|197266060\|ref\|ZP_03166133.1\| biotin-[acetyl-CoA-carboxylase] ligase [Salmonella enterica subsp. e...	297.0	[0..1]	[320..1]
gi\|110345905, gi... - gi\|110644313\|ref\|YP_672043.1\| biotin--protein ligase [Escherichia coli 536], gi\|110345905\|gb\|ABG7214... gi\|191172692, gi... - gi\|75240370\|ref\|ZP_00724310.1\| COG0340: Biotin-(acetyl-CoA carboxylase) ligase [Escherichia coli F11...	297.0	[0..1]	[321..1]
gi\|110807828, gi... - gi\|110807828\|ref\|YP_691348.1\| biotin--protein ligase [Shigella flexneri 5 str. 8401], gi\|110617376\|g...	296.0	[0..1]	[321..1]
gi\|126176359, gi... - gi\|68544816\|ref\|ZP_00584412.1\| Biotin--acetyl-CoA-carboxylase ligase [Shewanella baltica OS155], gi\|...	296.0	[0..1]	[319..1]
gi\|168752646, gi... - gi\|188013588\|gb\|EDU51710.1\| bifunctional protein BirA [Escherichia coli O157:H7 str. EC4113], gi\|168... gi\|168759132, gi... - gi\|189354243\|gb\|EDU72662.1\| bifunctional protein BirA [Escherichia coli O157:H7 str. EC4401], gi\|168... gi\|189364934, gi... - gi\|189364934\|gb\|EDU83350.1\| bifunctional protein BirA [Escherichia coli O157:H7 str. EC4501], gi\|168... gi\|189359776, gi... - gi\|189359776\|gb\|EDU78195.1\| bifunctional protein BirA [Escherichia coli O157:H7 str. EC4486], gi\|168... gi\|82778848, gi\|... - gi\|82778848\|ref\|YP_405197.1\| biotin--protein ligase [Shigella dysenteriae Sd197], gi\|81242996\|gb\|ABB... gi\|209751906, gi... - pir\|\|A86089 hypothetical protein birA [imported] - Escherichia coli (strain O157:H7, substrain EDL9... gi\|209161276, gi... - gi\|209399876\|ref\|YP_002273493.1\| bifunctional protein BirA [Escherichia coli O157:H7 str. EC4115], g... gi\|217322830, gi... - gi\|217326609\|ref\|ZP_03442693.1\| bifunctional protein BirA [Escherichia coli O157:H7 str. TW14588], g... gi\|12518899, gi\|... - gi\|15804567\|ref\|NP_290608.1\| biotin--protein ligase [Escherichia coli O157:H7 EDL933], gi\|12518899\|g... gi\|218707596, gi... - gi\|218707596\|ref\|YP_002415115.1\| biotin--protein ligase [Escherichia coli UMN026], gi\|218434693\|emb\|... gi\|13364376, gi\|... - gi\|15834154\|ref\|NP_312927.1\| biotin--protein ligase [Escherichia coli O157:H7], gi\|13364376\|dbj\|BAB3... gi\|254795976, gi... - gi\|254795976\|ref\|YP_003080813.1\| bifunctional biotin-[acetylCoA carboxylase] holoenzyme synthetase [... gi\|170520147, gi... - gi\|170682429\|ref\|YP_001746361.1\| bifunctional protein BirA [Escherichia coli SMS-3-5], gi\|170520147\|... gi\|208726089, gi... - gi\|208806288\|ref\|ZP_03248625.1\| bifunctional protein BirA [Escherichia coli O157:H7 str. EC4206], gi... gi\|208812148, gi... - gi\|208812148\|ref\|ZP_03253477.1\| bifunctional protein BirA [Escherichia coli O157:H7 str. EC4045], gi... gi\|13364376, gi\|... - gi\|15834154\|ref\|NP_312927.1\| biotin--protein ligase [Escherichia coli O157:H7 str. Sakai], gi\|133643... gi\|208739919, gi... - gi\|208819796\|ref\|ZP_03260116.1\| bifunctional protein BirA [Escherichia coli O157:H7 str. EC4042], gi... gi\|168778065, gi... - gi\|187766853\|gb\|EDU30697.1\| bifunctional protein BirA [Escherichia coli O157:H7 str. EC4196], gi\|168... gi\|168780898, gi... - gi\|189001543\|gb\|EDU70529.1\| bifunctional protein BirA [Escherichia coli O157:H7 str. EC4076], gi\|168... gi\|189373399, gi... - gi\|189373399\|gb\|EDU91815.1\| bifunctional protein BirA [Escherichia coli O157:H7 str. EC869], gi\|1687... gi\|168802900, gi... - gi\|189375264\|gb\|EDU93680.1\| bifunctional protein BirA [Escherichia coli O157:H7 str. EC508], gi\|1688...	296.0	[0..1]	[321..1]
gi\|170022019, gi... - gi\|170022019\|ref\|YP_001726973.1\| biotin--protein ligase [Escherichia coli ATCC 8739], gi\|169756947\|g... gi\|209921455, gi... - gi\|209921455\|ref\|YP_002295539.1\| biotin--protein ligase [Escherichia coli SE11], gi\|209914714\|dbj\|BA... gi\|82546319, gi\|... - gi\|82546319\|ref\|YP_410266.1\| biotin--protein ligase [Shigella boydii Sb227], gi\|81247730\|gb\|ABB68438... gi\|218697685, gi... - gi\|218697685\|ref\|YP_002405352.1\| biotin--protein ligase [Escherichia coli 55989], gi\|218354417\|emb\|C... gi\|124497995, gi... - gi\|124532580\|ref\|ZP_01702259.1\| bifunctional BirA, biotin operon repressor/biotin--acetyl-CoA-carbox... gi\|83584484, gi\|... - gi\|83584484\|ref\|ZP_00923166.1\| COG0340: Biotin-(acetyl-CoA carboxylase) ligase [Escherichia coli 101... gi\|75197842, gi\|... - gi\|75197842\|ref\|ZP_00707912.1\| COG0340: Biotin-(acetyl-CoA carboxylase) ligase [Escherichia coli HS]... gi\|251787235, gi... - gi\|251787235\|ref\|YP_003001539.1\| hypothetical protein B21_03807 [Escherichia coli BL21], gi\|24237950... gi\|75208235, gi\|... - gi\|75208235\|ref\|ZP_00708600.1\| COG0340: Biotin-(acetyl-CoA carboxylase) ligase [Escherichia coli B17... gi\|75176639, gi\|... - gi\|75176639\|ref\|ZP_00696767.1\| COG0340: Biotin-(acetyl-CoA carboxylase) ligase [Shigella boydii BS51... gi\|254163919, gi... - gi\|254163919\|ref\|YP_003047027.1\| biotin-protein ligase [Escherichia coli B str. REL606], gi\|25397582... gi\|157156012, gi... - gi\|75188708\|ref\|ZP_00701975.1\| COG0340: Biotin-(acetyl-CoA carboxylase) ligase [Escherichia coli E24... gi\|254290669, gi... - gi\|254290669\|ref\|YP_003056417.1\| biotin-protein ligase [Escherichia coli BL21(DE3)], gi\|253979976\|gb... gi\|75510802, gi\|... - gi\|75510802\|ref\|ZP_00733623.1\| COG0340: Biotin-(acetyl-CoA carboxylase) ligase [Escherichia coli 536... gi\|74314471, gi\|... - gi\|74314471\|ref\|YP_312890.1\| biotin--protein ligase [Shigella sonnei Ss046], gi\|73857948\|gb\|AAZ90655... gi\|193071829, gi... - gi\|75214529\|ref\|ZP_00713097.1\| COG0340: Biotin-(acetyl-CoA carboxylase) ligase [Escherichia coli E11... gi\|75226861, gi\|... - gi\|75226861\|ref\|ZP_00713766.1\| COG0340: Biotin-(acetyl-CoA carboxylase) ligase [Escherichia coli B7A...	296.0	[0..1]	[321..1]

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Predicted Domain #1

Region A:
Residues: [1-321]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKDNTVPLKL IALLANGEFH SGEQLGETLG MSRAAINKHI QTLRDWGVDV FTVPGKGYSL  60
   61 PEPIQLLNAK QILGQLDGGS VAVLPVIDST NQYLLDRIGE LKSGDACIAE YQQAGRGRRG 120
  121 RKWFSPFGAN LYLSMFWRLE QGPAAAIGLS LVIGIVMAEV LRKLGADKVR VKWPNDLYLQ 180
  181 DRKLAGILVE LTGKTGDAAQ IVIGAGINMA MRRVEESVVN QGWITLQEAG INLDRNTLAA 240
  241 MLIRELRAAL ELFEQEGLAP YLSRWEKLDN FINRPVKLII GDKEIFGISR GIDKQGALLL 300
  301 EQDGIIKPWM GGEISLRSAE K

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	80.221849
Match:	1biaA
Description:	THE E. COLI BIOTIN HOLOENZYME SYNTHETASE(SLASH)BIO REPRESSOR CRYSTAL STRUCTURE DELINEATES THE BIOTIN AND DNA-BINDING DOMAINS
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
ligase activity, forming carbon-oxygen bonds	3.33073922220594	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	3.33073922220594	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	3.33034630936933	bayes_pls_golite062009
ligase activity	3.05852492533937	bayes_pls_golite062009
catalytic activity	2.36416267096403	bayes_pls_golite062009
biotin-[acetyl-CoA-carboxylase] ligase activity	1.84290209862678	bayes_pls_golite062009
biotin-protein ligase activity	1.84290209862678	bayes_pls_golite062009
transcription activator activity	0.53294679084865	bayes_pls_golite062009
sequence-specific DNA binding	0.53285802828572	bayes_pls_golite062009
transcription regulator activity	0.514652697234715	bayes_pls_golite062009
nucleic acid binding	0.268902262140417	bayes_pls_golite062009
binding	0.204413263196103	bayes_pls_golite062009
DNA binding	0.114801541522161	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: