YRC Public Data Repository - Protein Structure Prediction

Protein:	BIOB_ECOLI
Organism:	Escherichia coli
Length:	346 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for BIOB_ECOLI.

Description	E-value	Query Range	Subject Range
BIOB_SHIB3 - Biotin synthase OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=bioB PE=3 SV=1 BIOB_SHIBS - Biotin synthase OS=Shigella boydii serotype 4 (strain Sb227) GN=bioB PE=3 SV=1	360.0	[0..1]	[346..1]
gi\|12620127 - gi\|12620127\|gb\|AAG60579.1\|AF250776_3 biotin synthase BioB [uncultured bacterium pCosHE2]	360.0	[0..1]	[346..1]
BIOB_ECOL6 - Biotin synthase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) GN=bioB PE=3 SV=1 BIOB_ECO45 - Biotin synthase OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=bioB PE=3 SV=1 BIOB_ECO81 - Biotin synthase OS=Escherichia coli O81 (strain ED1a) GN=bioB PE=3 SV=1 BIOB_ECOL6 - Biotin synthase OS=Escherichia coli O6 GN=bioB PE=3 SV=1 gi\|237707269, gi... - gi\|237707269\|ref\|ZP_04537750.1\| biotin synthase [Escherichia sp. 3_2_53FAA], gi\|226898479\|gb\|EEH8473... gi\|227839015, gi... - gi\|227884263\|ref\|ZP_04002068.1\| biotin synthase [Escherichia coli 83972], gi\|227839015\|gb\|EEJ49481.1... BIOB_ECOK1 - Biotin synthase OS=Escherichia coli O1:K1 / APEC GN=bioB PE=3 SV=1 BIOB_ECOUT - Biotin synthase OS=Escherichia coli (strain UTI89 / UPEC) GN=bioB PE=3 SV=1	360.0	[0..1]	[346..1]
gi\|25294055 - pir\|\|F85585 biotin synthesis, sulfur insertion [imported] - Escherichia coli (strain O157:H7, substr... gi\|12513761, gi\|... - gi\|15800526\|ref\|NP_286538.1\| biotin synthase [Escherichia coli O157:H7 EDL933], gi\|12513761\|gb\|AAG55...	359.0	[0..1]	[346..1]
gi\|208808075, gi... - gi\|208808075\|ref\|ZP_03250412.1\| biotin synthase [Escherichia coli O157:H7 str. EC4206], gi\|208727876... gi\|195936813 - gi\|195936813\|ref\|ZP_03082195.1\| biotin synthase [Escherichia coli O157:H7 str. EC4024] gi\|168786986, gi... - gi\|189373194\|gb\|EDU91610.1\| biotin synthase [Escherichia coli O157:H7 str. EC869], gi\|168786986\|ref\|... gi\|168779316, gi... - gi\|189002816\|gb\|EDU71802.1\| biotin synthase [Escherichia coli O157:H7 str. EC4076], gi\|168779316\|ref... gi\|217322605, gi... - gi\|217326384\|ref\|ZP_03442468.1\| biotin synthase [Escherichia coli O157:H7 str. TW14588], gi\|21732260... BIOB_ECO5E - Biotin synthase OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=bioB PE=3 SV=1 gi\|208822295, gi... - gi\|208822295\|ref\|ZP_03262614.1\| biotin synthase [Escherichia coli O157:H7 str. EC4042], gi\|208737780... gi\|208815801, gi... - gi\|208815801\|ref\|ZP_03256980.1\| biotin synthase [Escherichia coli O157:H7 str. EC4045], gi\|208732449... gi\|168776427, gi... - gi\|187768146\|gb\|EDU31990.1\| biotin synthase [Escherichia coli O157:H7 str. EC4196], gi\|168776427\|ref... gi\|168763417, gi... - gi\|189366479\|gb\|EDU84895.1\| biotin synthase [Escherichia coli O157:H7 str. EC4501], gi\|168763417\|ref... gi\|168757116, gi... - gi\|189355846\|gb\|EDU74265.1\| biotin synthase [Escherichia coli O157:H7 str. EC4401], gi\|168757116\|ref... gi\|168750320, gi... - gi\|188015512\|gb\|EDU53634.1\| biotin synthase [Escherichia coli O157:H7 str. EC4113], gi\|168750320\|ref... gi\|254591369, gi... - gi\|254791969\|ref\|YP_003076806.1\| biotin synthase [Escherichia coli O157:H7 str. TW14359], gi\|2545913... gi\|15830107, gi\|... - gi\|15830107\|ref\|NP_308880.1\| biotin synthase [Escherichia coli O157:H7 str. Sakai], gi\|13360312\|dbj\|... gi\|209776122, gi... - pir\|\|E90735 biotin synthetase [imported] - Escherichia coli (strain O157:H7, substrain RIMD 0509952)... BIOB_ECO57 - Biotin synthase OS=Escherichia coli O157:H7 GN=bioB PE=3 SV=3	359.0	[0..1]	[346..1]
gi\|190905542, gi... - gi\|75237111\|ref\|ZP_00721165.1\| COG0502: Biotin synthase and related enzymes [Escherichia coli F11], ... BIOB_ECOL5 - Biotin synthase OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=bioB PE=3 SV=1	358.0	[0..1]	[346..1]
gi\|42543471, gi\|... - gi\|42543471\|pdb\|1R30\|B Chain B, The Crystal Structure Of Biotin Synthase, An S- Adenosylmethionine-D...	358.0	[0..2]	[346..25]
BIOB_SHIDS - Biotin synthase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=bioB PE=3 SV=1	357.0	[0..1]	[346..1]
gi\|85693260, gi\|... - gi\|85712880\|ref\|ZP_01043921.1\| Biotin synthase [Idiomarina baltica OS145], gi\|85693260\|gb\|EAQ31217.1...	356.0	[0..2]	[344..8]

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Predicted Domain #1

Region A:
Residues: [1-346]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAHRPRWTLS QVTELFEKPL LDLLFEAQQV HRQHFDPRQV QVSTLLSIKT GACPEDCKYC  60
   61 PQSSRYKTGL EAERLMEVEQ VLESARKAKA AGSTRFCMGA AWKNPHERDM PYLEQMVQGV 120
  121 KAMGLEACMT LGTLSESQAQ RLANAGLDYY NHNLDTSPEF YGNIITTRTY QERLDTLEKV 180
  181 RDAGIKVCSG GIVGLGETVK DRAGLLLQLA NLPTPPESVP INMLVKVKGT PLADNDDVDA 240
  241 FDFIRTIAVA RIMMPTSYVR LSAGREQMNE QTQAMCFMAG ANSIFYGCKL LTTPNPEEDK 300
  301 DLQLFRKLGL NPQQTAVLAG DNEQQQRLEQ ALMTPDTDEY YNAAAL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	99.0
Match:	1r30A
Description:	The Crystal Structure of Biotin Synthase, an S-Adenosylmethionine-Dependent Radical Enzyme
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
biotin synthase activity	4.96431653338581	bayes_pls_golite062009
radical SAM enzyme activity	4.81217611621334	bayes_pls_golite062009
sulfurtransferase activity	3.43375708969779	bayes_pls_golite062009
transferase activity, transferring sulfur-containing groups	2.79703059227143	bayes_pls_golite062009
coproporphyrinogen oxidase activity	1.98937742831419	bayes_pls_golite062009
catalytic activity	1.48331922124015	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-CH group of donors, oxygen as acceptor	1.37188743230327	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-CH group of donors	1.18901545014659	bayes_pls_golite062009
oxidoreductase activity	0.593156857804038	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: