YRC Public Data Repository - Protein Structure Prediction

Protein:	BARA_ECOLI
Organism:	Escherichia coli
Length:	918 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for BARA_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|24114070, gi\|... - gi\|24114070\|ref\|NP_708580.1\| hybrid sensory histidine kinase BarA [Shigella flexneri 2a str. 301], g... gi\|30064131, gi\|... - gi\|30064131\|ref\|NP_838302.1\| hybrid sensory histidine kinase BarA [Shigella flexneri 2a str. 2457T],... BARA_SHIFL - Signal transduction histidine-protein kinase BarA OS=Shigella flexneri GN=barA PE=3 SV=1	567.0	[0..1]	[918..1]
gi\|110642927, gi... - gi\|110642927\|ref\|YP_670657.1\| hybrid sensory histidine kinase BarA [Escherichia coli 536], gi\|110344... gi\|190908466, gi... - gi\|75241824\|ref\|ZP_00725643.1\| COG0642: Signal transduction histidine kinase [Escherichia coli F11],...	566.0	[0..1]	[918..1]
gi\|82545085, gi\|... - gi\|82545085\|ref\|YP_409032.1\| hybrid sensory histidine kinase BarA [Shigella boydii Sb227], gi\|812464...	566.0	[0..1]	[918..1]
gi\|25308230 - gi\|25308230\|pir\|\|G85929 hypothetical protein barA [imported] - Escherichia coli (strain O157:H7, su... gi\|12517258, gi\|... - gi\|15803307\|ref\|NP_289340.1\| hybrid sensory histidine kinase BarA [Escherichia coli O157:H7 EDL933],...	566.0	[0..1]	[918..1]
gi\|75256875, gi\|... - gi\|75256875\|ref\|ZP_00728469.1\| COG0642: Signal transduction histidine kinase [Escherichia coli E22],...	565.0	[0..1]	[918..1]
gi\|237706586, gi... - gi\|237706586\|ref\|ZP_04537067.1\| sensor-regulator [Escherichia sp. 3_2_53FAA], gi\|226899626\|gb\|EEH858... gi\|91212153, gi\|... - gi\|91212153\|ref\|YP_542139.1\| hybrid sensory histidine kinase BarA [Escherichia coli UTI89], gi\|91073... gi\|218366543, gi... - gi\|218559774\|ref\|YP_002392687.1\| hybrid sensory histidine kinase BarA [Escherichia coli S88], gi\|218... gi\|218428473, gi... - gi\|218690909\|ref\|YP_002399121.1\| hybrid sensory histidine kinase BarA [Escherichia coli ED1a], gi\|21... gi\|117625014, gi... - gi\|117625014\|ref\|YP_854002.1\| hybrid sensory histidine kinase BarA [Escherichia coli APEC O1], gi\|11...	565.0	[0..1]	[918..1]
gi\|110616096, gi... - gi\|110806548\|ref\|YP_690068.1\| hybrid sensory histidine kinase BarA [Shigella flexneri 5 str. 8401], ...	564.0	[0..1]	[918..1]
gi\|204928346, gi... - gi\|204928346\|ref\|ZP_03219546.1\| conserved hypothetical protein [Salmonella enterica subsp. enterica ... gi\|224584749, gi... - gi\|224584749\|ref\|YP_002638547.1\| sensor protein [Salmonella enterica subsp. enterica serovar Paratyp... gi\|194403303, gi... - gi\|194444640\|ref\|YP_002042209.1\| signal transduction histidine-protein kinase BarA [Salmonella enter... gi\|194471010, gi... - gi\|194471010\|ref\|ZP_03076994.1\| signal transduction histidine-protein kinase BarA [Salmonella enteri... gi\|168463891, gi... - gi\|195633256\|gb\|EDX51670.1\| signal transduction histidine-protein kinase BarA [Salmonella enterica s... gi\|168820322, gi... - gi\|205342849\|gb\|EDZ29613.1\| signal transduction histidine-protein kinase BarA [Salmonella enterica s... gi\|205334634, gi... - gi\|205334634\|gb\|EDZ21398.1\| conserved hypothetical protein [Salmonella enterica subsp. enterica sero... gi\|168261898, gi... - gi\|205349206\|gb\|EDZ35837.1\| signal transduction histidine-protein kinase BarA [Salmonella enterica s... gi\|167550176, gi... - gi\|205324815\|gb\|EDZ12654.1\| signal transduction histidine-protein kinase BarA [Salmonella enterica s... gi\|6996656\|gb\|AA... - BarA sensor kinase [Salmonella typhimurium] gi\|205328470, gi... - gi\|205328470\|gb\|EDZ15234.1\| signal transduction histidine-protein kinase BarA [Salmonella enterica s... gi\|161365250, gi... - gi\|161615886\|ref\|YP_001589851.1\| hybrid sensory histidine kinase BarA [Salmonella enterica subsp. en... gi\|16766264, gi\|... - gi\|16766264\|ref\|NP_461879.1\| hybrid sensory histidine kinase BarA [Salmonella typhimurium LT2], gi\|1... gi\|238909738 - gi\|238909738\|ref\|ZP_04653575.1\| hybrid sensory histidine kinase BarA [Salmonella enterica subsp. ent... gi\|6996656 - gi\|6996656\|gb\|AAF34823.1\|AF171069_1 BarA sensor kinase [Salmonella typhimurium] lcl\|NC_016856.1_... - [gene=barA] [protein=hybrid sensory histidine kinase BarA] [protein_id=YP_005239075.1] [location=312...	564.0	[0..1]	[918..1]
gi\|62129101, gi\|... - gi\|62181468\|ref\|YP_217885.1\| hybrid sensory histidine kinase BarA [Salmonella enterica subsp. enteri...	564.0	[0..1]	[918..1]
gi\|197095315, gi... - gi\|197363838\|ref\|YP_002143475.1\| hybrid sensory histidine kinase BarA [Salmonella enterica subsp. en... gi\|194409907, gi... - gi\|194451603\|ref\|YP_002046927.1\| hybrid sensory histidine kinase BarA [Salmonella enterica subsp. en... gi\|205338112, gi... - gi\|205338112\|gb\|EDZ24876.1\| conserved hypothetical protein [Salmonella enterica subsp. enterica sero... gi\|56414910, gi\|... - gi\|56414910\|ref\|YP_151985.1\| hybrid sensory histidine kinase BarA [Salmonella enterica subsp. enteri...	563.0	[0..1]	[918..1]

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Predicted Domain #1

Region A:
Residues: [1-150]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTNYSLRARM MILILAPTVL IGLLLSIFFV VHRYNDLQRQ LEDAGASIIE PLAVSTEYGM  60
   61 SLQNRESIGQ LISVLHRRHS DIVRAISVYD ENNRLFVTSN FHLDPSSMQL GSNVPFPRQL 120
  121 TVTRDGDIMI LRTPIISESY SPDESPSSDA

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [151-270]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KNSQNMLGYI ALELDLKSVR LQQYKEIFIS SVMMLFCIGI ALIFGWRLMR DVTGPIRNMV  60
   61 NTVDRIRRGQ LDSRVEGFML GELDMLKNGI NSMAMSLAAY HEEMQHNIDQ ATSDLRETLE 120
  121

[Run NCBI BLAST on this sequence.]

Detection Method:	FFAS03
Confidence:	3.8
Match:	2f95B
Description:	M intermediate structure of sensory rhodopsin II/transducer complex in combination with the ground state structure
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [271-527]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QMEIQNVELD LAKKRAQEAA RIKSEFLANM SHELRTPLNG VIGFTRLTLK TELTPTQRDH  60
   61 LNTIERSANN LLAIINDVLD FSKLEAGKLI LESIPFPLRS TLDEVVTLLA HSSHDKGLEL 120
  121 TLNIKSDVPD NVIGDPLRLQ QIITNLVGNA IKFTENGNID ILVEKRALSN TKVQIEVQIR 180
  181 DTGIGIPERD QSRLFQAFRQ ADASISRRHG GTGLGLVITQ KLVNEMGGDI SFHSQPNRGS 240
  241 TFWFHINLDL NPNIIIE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	49.30103
Match:	2c2aA
Description:	Structure of the entire cytoplasmic portion of a sensor histidine kinase protein
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [528-918]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GPSTQCLAGK RLAYVEPNSA AAQCTLDILS ETPLEVVYSP TFSALPPAHY DMMLLGIAVT  60
   61 FREPLTMQHE RLAKAVSMTD FLMLALPCHA QVNAEKLKQD GIGACLLKPL TPTRLLPALT 120
  121 EFCHHKQNTL LPVTDESKLA MTVMAVDDNP ANLKLIGALL EDMVQHVELC DSGHQAVERA 180
  181 KQMPFDLILM DIQMPDMDGI RACELIHQLP HQQQTPVIAV TAHAMAGQKE KLLGAGMSDY 240
  241 LAKPIEEERL HNLLLRYKPG SGISSRVVTP EVNEIVVNPN ATLDWQLALR QAAGKTDLAR 300
  301 DMLQMLLDFL PEVRNKVEEQ LVGENPEGLV DLIHKLHGSC GYSGVPRMKN LCQLIEQQLR 360
  361 SGTKEEDLEP ELLELLDEMD NVAREASKIL G

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	30.0
Match:	1w25A
Description:	Response regulator PleD in complex with c-diGMP
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
two-component response regulator activity	7.02872640261738	bayes_pls_golite062009
signal transducer activity	4.51666042720644	bayes_pls_golite062009
molecular transducer activity	4.51666042720644	bayes_pls_golite062009
transcription regulator activity	3.44888100104099	bayes_pls_golite062009
protein histidine kinase activity	2.35011582062573	bayes_pls_golite062009
two-component sensor activity	2.32303301074957	bayes_pls_golite062009
phosphotransferase activity, nitrogenous group as acceptor	2.28838869071567	bayes_pls_golite062009
binding	1.22427537074115	bayes_pls_golite062009
DNA binding	1.11037597107903	bayes_pls_golite062009
protein kinase activity	1.00737355751913	bayes_pls_golite062009
nucleic acid binding	0.93151849951839	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.811771244977462	bayes_pls_golite062009
kinase activity	0.725824770559857	bayes_pls_golite062009
transcription factor activity	0.62886970109503	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.476057209677045	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: