YRC Public Data Repository - Protein Structure Prediction

Protein:	AMY2_ECOLI
Organism:	Escherichia coli
Length:	495 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for AMY2_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|238784693, gi... - gi\|77957959\|ref\|ZP_00822002.1\| COG0366: Glycosidases [Yersinia bercovieri ATCC 43970], gi\|238784693\|...	324.0	[0..1]	[495..1]
gi\|77979886, gi\|... - gi\|77979886\|ref\|ZP_00835302.1\| COG0366: Glycosidases [Yersinia intermedia ATCC 29909], gi\|238794046\|...	319.0	[0..1]	[495..1]
gi\|238788404, gi... - gi\|77975634\|ref\|ZP_00831168.1\| COG0366: Glycosidases [Yersinia frederiksenii ATCC 33641], gi\|2387884...	315.0	[0..1]	[495..1]
gi\|56413124, gi\|... - gi\|56413124\|ref\|YP_150199.1\| cytoplasmic alpha-amylase [Salmonella enterica subsp. enterica serovar ... gi\|197362050, gi... - gi\|197362050\|ref\|YP_002141687.1\| cytoplasmic alpha-amylase [Salmonella enterica subsp. enterica sero...	306.0	[0..1]	[494..1]
gi\|23493851, gi\|... - gi\|25028566\|ref\|NP_738620.1\| putative glycogen debranching enzyme [Corynebacterium efficiens YS-314]...	306.0	[0..3]	[490..164]
gi\|16760907 - gi\|16760907\|ref\|NP_456524.1\| cytoplasmic alpha-amylase [Salmonella enterica subsp. enterica serovar ... gi\|213426347 - gi\|213426347\|ref\|ZP_03359097.1\| cytoplasmic alpha-amylase [Salmonella enterica subsp. enterica serov... gi\|213855238 - gi\|213855238\|ref\|ZP_03383478.1\| cytoplasmic alpha-amylase [Salmonella enterica subsp. enterica serov... gi\|25289305, gi\|... - gi\|25289305\|pir\|\|AD0751 cytoplasmic alpha-amylase [imported] - Salmonella enterica subsp. enterica s... gi\|29141401, gi\|... - gi\|29141401\|ref\|NP_804743.1\| cytoplasmic alpha-amylase [Salmonella enterica subsp. enterica serovar ... gi\|213053502 - gi\|213053502\|ref\|ZP_03346380.1\| cytoplasmic alpha-amylase [Salmonella enterica subsp. enterica serov...	305.0	[0..1]	[494..1]
gi\|25289307 - pir\|\|AD3038 alpha-amylase amyA [imported] - Agrobacterium tumefaciens (strain C58, Dupont) gi\|159141081, gi... - gi\|17937617\|ref\|NP_534406.1\| cytoplasmic alpha-amylase [Agrobacterium tumefaciens str. C58], gi\|1774...	304.0	[0..1]	[494..1]
AMY2_SALTY - Cytoplasmic alpha-amylase OS=Salmonella typhimurium GN=amyA PE=3 SV=3 lcl\|NC_016856.1_... - [gene=amyA] [protein=alpha-amylase] [protein_id=YP_005237932.1] [location=2063072..2064556] gi\|167993536, gi... - gi\|205328404\|gb\|EDZ15168.1\| cytoplasmic alpha-amylase [Salmonella enterica subsp. enterica serovar 4... AMY2_SALTY - Cytoplasmic alpha-amylase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) GN=amyA PE... AMY2_SALTY - Cytoplasmic alpha-amylase OS=Salmonella typhimurium GN=amyA PE=3 SV=3	304.0	[0..1]	[494..1]
gi\|62128170, gi\|... - gi\|62180537\|ref\|YP_216954.1\| cytoplasmic alpha-amylase [Salmonella enterica subsp. enterica serovar ...	303.0	[0..1]	[494..1]

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Predicted Domain #1

Region A:
Residues: [1-495]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MRNPTLLQCF HWYYPEGGKL WPELAERADG FNDIGINMVW LPPAYKGASG GYSVGYDSYD  60
   61 LFDLGEFDQK GSIPTKYGDK AQLLAAIDAL KRNDIAVLLD VVVNHKMGAD EKEAIRVQRV 120
  121 NADDRTQIDE EIIECEGWTR YTFPARAGQY SQFIWDFKCF SGIDHIENPD EDGIFKIVND 180
  181 YTGEGWNDQV DDELGNFDYL MGENIDFRNH AVTEEIKYWA RWVMEQTQCD GFRLDAVKHI 240
  241 PAWFYKEWIE HVQEVAPKPL FIVAEYWSHE VDKLQTYIDQ VEGKTMLFDA PLQMKFHEAS 300
  301 RMGRDYDMTQ IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEPWFKPL AYALILLREN 360
  361 GVPSVFYPDL YGAHYEDVGG DGQTYPIDMP IIEQLDELIL ARQRFAHGVQ TLFFDHPNCI 420
  421 AFSRSGTDEF PGCVVVMSNG DDGEKTIHLG ENYGNKTWRD FLGNRQERVV TDENGEATFF 480
  481 CNGGSVSVWV IEEVI

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	77.39794
Match:	1e3xA
Description:	Bacterial alpha-Amylase; Bacterial alpha-amylase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
hydrolase activity, acting on glycosyl bonds	3.1190602686215	bayes_pls_golite062009
hydrolase activity, hydrolyzing O-glycosyl compounds	2.98773241907716	bayes_pls_golite062009
catalytic activity	2.22613067995402	bayes_pls_golite062009
hydrolase activity	1.107656067558	bayes_pls_golite062009
glucosidase activity	0.58717079348538	bayes_pls_golite062009
hexosaminidase activity	0.16438321606077	bayes_pls_golite062009
amylase activity	0.0914002140078796	bayes_pls_golite062009
alpha-amylase activity	0.0521244165288097	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: