YRC Public Data Repository - Protein Structure Prediction

Protein:	AMY1_ECOLI
Organism:	Escherichia coli
Length:	676 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for AMY1_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|110617310, gi... - gi\|110807762\|ref\|YP_691282.1\| periplasmic alpha-amylase precursor [Shigella flexneri 5 str. 8401], g...	447.0	[0..1]	[671..1]
gi\|194435960, gi... - gi\|83588575\|ref\|ZP_00927199.1\| COG0366: Glycosidases [Escherichia coli 101-1], gi\|194435960\|ref\|ZP_0...	446.0	[0..1]	[671..1]
gi\|193068583, gi... - gi\|75237508\|ref\|ZP_00721541.1\| COG0366: Glycosidases [Escherichia coli E110019], gi\|193068583\|ref\|ZP...	446.0	[0..1]	[671..1]
gi\|55607441 - gb\|AAV54069.1\| alpha-amylase [Escherichia coli] gi\|55607441 - gi\|55607441\|gb\|AAV54069.1\| alpha-amylase [Escherichia coli]	445.0	[0..1]	[671..1]
gi\|91074660, gi\|... - gi\|91213086\|ref\|YP_543072.1\| periplasmic alpha-amylase precursor [Escherichia coli UTI89], gi\|910746... gi\|218367417, gi... - gi\|218560648\|ref\|YP_002393561.1\| periplasmic alpha-amylase precursor [Escherichia coli S88], gi\|2183... gi\|237703343, gi... - gi\|237703343\|ref\|ZP_04533824.1\| periplasmic alpha-amylase MalS [Escherichia sp. 3_2_53FAA], gi\|22690... gi\|117625854, gi... - gi\|117625854\|ref\|YP_859177.1\| periplasmic alpha-amylase precursor [Escherichia coli APEC O1], gi\|115...	445.0	[0..1]	[671..1]
gi\|193061783, gi... - gi\|75260140\|ref\|ZP_00731408.1\| COG0366: Glycosidases [Escherichia coli E22], gi\|193061783\|ref\|ZP_030... gi\|191165322, gi... - gi\|75231584\|ref\|ZP_00717942.1\| COG0366: Glycosidases [Escherichia coli B7A], gi\|191165322\|ref\|ZP_030... gi\|75211325, gi\|... - gi\|75211325\|ref\|ZP_00711424.1\| COG0366: Glycosidases [Escherichia coli B171], gi\|194427456\|ref\|ZP_03...	445.0	[0..1]	[671..1]
gi\|30065144, gi\|... - gi\|30065144\|ref\|NP_839315.1\| periplasmic alpha-amylase precursor [Shigella flexneri 2a str. 2457T], ... gi\|24114846, gi\|... - gi\|24114846\|ref\|NP_709356.1\| periplasmic alpha-amylase precursor [Shigella flexneri 2a str. 301], gi...	445.0	[0..1]	[671..1]
gi\|217320635, gi... - gi\|217324414\|ref\|ZP_03440498.1\| alpha-amylase, periplasmic [Escherichia coli O157:H7 str. TW14588], ... gi\|208728275, gi... - gi\|208808474\|ref\|ZP_03250811.1\| alpha-amylase, periplasmic [Escherichia coli O157:H7 str. EC4206], g... gi\|208812665, gi... - gi\|208812665\|ref\|ZP_03253994.1\| alpha-amylase, periplasmic [Escherichia coli O157:H7 str. EC4045], g... gi\|208819748, gi... - gi\|208819748\|ref\|ZP_03260068.1\| alpha-amylase, periplasmic [Escherichia coli O157:H7 str. EC4042], g... gi\|209161058, gi... - gi\|209399658\|ref\|YP_002273057.1\| alpha-amylase, periplasmic [Escherichia coli O157:H7 str. EC4115], ... gi\|189002436, gi... - gi\|189002436\|gb\|EDU71422.1\| alpha-amylase, periplasmic [Escherichia coli O157:H7 str. EC4076], gi\|16... gi\|189374310, gi... - gi\|189374310\|gb\|EDU92726.1\| alpha-amylase, periplasmic [Escherichia coli O157:H7 str. EC869], gi\|168... gi\|168797410, gi... - gi\|189379873\|gb\|EDU98289.1\| alpha-amylase, periplasmic [Escherichia coli O157:H7 str. EC508], gi\|168... gi\|195935103 - gi\|195935103\|ref\|ZP_03080485.1\| periplasmic alpha-amylase precursor [Escherichia coli O157:H7 str. E... gi\|189359261, gi... - gi\|189359261\|gb\|EDU77680.1\| alpha-amylase, periplasmic [Escherichia coli O157:H7 str. EC4401], gi\|16... gi\|168759664, gi... - gi\|189369836\|gb\|EDU88252.1\| alpha-amylase, periplasmic [Escherichia coli O157:H7 str. EC4501], gi\|16... gi\|168765987, gi... - gi\|189364378\|gb\|EDU82797.1\| alpha-amylase, periplasmic [Escherichia coli O157:H7 str. EC4486], gi\|16... gi\|187771495, gi... - gi\|187771495\|gb\|EDU35339.1\| alpha-amylase, periplasmic [Escherichia coli O157:H7 str. EC4196], gi\|16... gi\|168746882, gi... - gi\|188018420\|gb\|EDU56542.1\| alpha-amylase, periplasmic [Escherichia coli O157:H7 str. EC4113], gi\|16... gi\|209755080, gi... - pir\|\|F91185 alpha-amylase [imported] - Escherichia coli (strain O157:H7, substrain RIMD 0509952), gi... gi\|13363928, gi\|... - gi\|15833708\|ref\|NP_312481.1\| periplasmic alpha-amylase precursor [Escherichia coli O157:H7], gi\|1336... gi\|254594928, gi... - gi\|254795528\|ref\|YP_003080365.1\| alpha-amylase [Escherichia coli O157:H7 str. TW14359], gi\|254594928... gi\|13363928, gi\|... - gi\|15833708\|ref\|NP_312481.1\| periplasmic alpha-amylase precursor [Escherichia coli O157:H7 str. Saka...	444.0	[0..1]	[671..1]
gi\|218556132, gi... - gi\|218556132\|ref\|YP_002389045.1\| periplasmic alpha-amylase precursor [Escherichia coli IAI1], gi\|218... gi\|157158372, gi... - gi\|75188335\|ref\|ZP_00701602.1\| COG0366: Glycosidases [Escherichia coli E24377A], gi\|157158372\|ref\|YP...	444.0	[0..1]	[671..1]
gi\|81247353, gi\|... - gi\|82545942\|ref\|YP_409889.1\| periplasmic alpha-amylase precursor [Shigella boydii Sb227], gi\|8124735...	444.0	[0..1]	[671..1]

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Predicted Domain #1

Region A:
Residues: [1-676]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKLAACFLTL LPGFAVAASW TSPGFPAFSE QGTGTFVSHA QLPKGTRPLT LNFDQQCWQP  60
   61 ADAIKLNQML SLQPCSNTPP QWRLFRDGEY TLQIDTRSGT PTLMISIQNA AEPVASLVRE 120
  121 CPKWDGLPLT VDVSATFPEG AAVRDYYSQQ IAIVKNGQIM LQPAATSNGL LLLERAETDT 180
  181 SAPFDWHNAT VYFVLTDRFE NGDPSNDQSY GRHKDGMAEI GTFHGGDLRG LTNKLDYLQQ 240
  241 LGVNALWISA PFEQIHGWVG GGTKGDFPHY AYHGYYTQDW TNLDANMGNE ADLRTLVDSA 300
  301 HQRGIRILFD VVMNHTGYAT LADMQEYQFG ALYLSGDEVK KSLGERWSDW KPAAGQTWHS 360
  361 FNDYINFSDK TGWDKWWGKN WIRTDIGDYD NPGFDDLTMS LAFLPDIKTE STTASGLPVF 420
  421 YKNKMDTHAK AIDGYTPRDY LTHWLSQWVR DYGIDGFRVD TAKHVELPAW QQLKTEASAA 480
  481 LREWKKANPD KALDDKPFWM TGEAWGHGVM QSDYYRHGFD AMINFDYQEQ AAKAVDCLAQ 540
  541 MDTTWQQMAE KLQGFNVLSY LSSHDTRLFR EGGDKAAELL LLAPGAVQIF YGDESSRPFG 600
  601 PTGSDPLQGT RSDMNWQDVS GKSAASVAHW QKISQFRARH PAIGAGKQTT LLLKQGYGFV 660
  661 REHGDDKVLV VWAGQQ

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	52.522879
Match:	1wzkA
Description:	Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) mutatnt D465N
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
hydrolase activity, acting on glycosyl bonds	3.1190602686215	bayes_pls_golite062009
hydrolase activity, hydrolyzing O-glycosyl compounds	2.98773241907716	bayes_pls_golite062009
catalytic activity	2.22613067995402	bayes_pls_golite062009
hydrolase activity	1.97740088175981	bayes_pls_golite062009
glucosidase activity	0.58717079348538	bayes_pls_golite062009
hexosaminidase activity	0.16438321606077	bayes_pls_golite062009
amylase activity	0.0914002140078796	bayes_pls_golite062009
alpha-amylase activity	0.0521244165288097	bayes_pls_golite062009
chitinase activity	0.0382131529139698	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: