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View Structure Prediction Details

Protein: ALF1
Organism: Saccharomyces cerevisiae
Length: 254 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for ALF1.

Description E-value Query
Range
Subject
Range
TBCB_YEAST - Tubulin-specific chaperone B OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ALF1 PE=1 S...
ALF1 - Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic m...
gi|190409134 - gi|190409134|gb|EDV12399.1| tubulin folding cofactor B [Saccharomyces cerevisiae RM11-1a]
2.0E-70 [1..254] [1..254]
TBCB_MOUSE - Tubulin-folding cofactor B OS=Mus musculus GN=Tbcb PE=1 SV=2
4.0E-66 [2..244] [11..244]
gi|7507776 - gi|7507776|pir||T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans
3.0E-57 [2..254] [4..240]
ALP11_SCHPO - Cell polarity protein alp11 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=alp11 PE=1 SV=...
alp11 - tubulin specific chaperone cofactor B
1.0E-54 [2..238] [4..229]
CLIP3 - CAP-GLY domain containing linker protein 3
2.0E-54 [17..245] [283..502]

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Predicted Domain #1
Region A:
Residues: [1-142]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVRVVIESEL VRTEKELPNS LKLRQFKDRL YHVTGVEPED MEIVVKRQYD NKEIYSTKKG  60
   61 GAYSNEDEDA NFLKGEEELI VVVTDSNAQS ISNQLATQAE GIPSMEVISE EDYLRRDQSV 120
  121 LRWKMAHGYG RFNAAQQSQR AA

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

    MCM
Score
GO
Score
GO
Term
SCOP
Match
SCOP Description
View Download 0.284 0.257 post-chaperonin tubulin folding pathway d.15.1 Ubiquitin-like
View Download 0.305 0.094 post-chaperonin tubulin folding pathway d.109.1 Actin depolymerizing proteins
View Download 0.228 0.035 post-chaperonin tubulin folding pathway a.11.2 Second domain of FERM
View Download 0.226 0.010 post-chaperonin tubulin folding pathway d.82.2 Frataxin-like
View Download 0.420 N/A N/A d.87.1 FAD/NAD-linked reductases, dimerisation (C-terminal) domain

Predicted functions:

Term Confidence Notes
binding 2.19381585790185 bayes_pls_golite062009
protein binding 1.17197911794381 bayes_pls_golite062009
structural constituent of ribosome 0.599634454232833 bayes_pls_golite062009
structural molecule activity 0.576819726104993 bayes_pls_golite062009
nucleic acid binding 0.383121378043805 bayes_pls_golite062009
DNA binding 0.0840213088061073 bayes_pls_golite062009
transcription regulator activity 0.0485636215870291 bayes_pls_golite062009

Predicted Domain #2
Region A:
Residues: [143-254]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LAKQDEAYAR EQLTAAIGRH CRVTVDGSAP REAILRYVGP LPLDVMGTWC GVEFPEAAGK  60
   61 NDGRINGVTL FGPVAPGHGS FVRPRAVEIL SKDEESAEVE DVHDDVESDD EI

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 94.221849
Match: 1lplA_
Description: Cytoskeleton-associated protein F53F4.3
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
tubulin binding 2.81573953488242 bayes_pls_golite062009
microtubule binding 2.67824962486294 bayes_pls_golite062009
binding 2.07863891614262 bayes_pls_golite062009
cytoskeletal protein binding 1.95334135428526 bayes_pls_golite062009
microtubule plus-end binding 1.16043967945651 bayes_pls_golite062009
protein binding 0.896264971703612 bayes_pls_golite062009
nucleic acid binding 0.625661567957308 bayes_pls_golite062009
DNA binding 0.336599045092266 bayes_pls_golite062009
transcription regulator activity 0.292990934444865 bayes_pls_golite062009
catalytic activity 0.069855517589921 bayes_pls_golite062009

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle