YRC Public Data Repository - Protein Structure Prediction

Protein:	ECM4
Organism:	Saccharomyces cerevisiae
Length:	370 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for ECM4.

Description	E-value	Query Range	Subject Range
GTO2_YEAST - Glutathione S-transferase omega-like 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=E... gi\|151941615 - gi\|151941615\|gb\|EDN59978.1\| conserved protein [Saccharomyces cerevisiae YJM789] ECM4 - Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (... gi\|190409888 - gi\|190409888\|gb\|EDV13153.1\| extracellular matrix protein 4 [Saccharomyces cerevisiae RM11-1a]	0.0	[1..370]	[1..370]
gi\|25346667 - pir\|\|A97631 hypothetical 37.4K protein in exuR-tdcC intergenic region (o328) [imported] - Agrobacte... gi\|15889536, gi\|... - gi\|15889536\|ref\|NP_355217.1\| hypothetical protein AGR_C_4109 [Agrobacterium tumefaciens str. C58], g...	0.0	[6..367]	[72..376]
gi\|7469828 - pir\|\|S76960 hypothetical protein - Synechocystis sp. (strain PCC 6803) gi\|1653962, gi\|1... - gi\|1653962\|dbj\|BAA18872.1\| slr0605 [Synechocystis sp. PCC 6803], gi\|16332332\|ref\|NP_443060.1\| hypoth...	0.0	[6..368]	[16..317]
gi\|16762005 - gi\|16762005\|ref\|NP_457622.1\| hypothetical protein STY3413 [Salmonella enterica subsp. enterica serov... gi\|16504308, gi\|... - gi\|25346664\|pir\|\|AG0895 conserved hypothetical protein STY3413 [imported] - Salmonella enterica subs... gi\|29139126, gi\|... - gi\|29143492\|ref\|NP_806834.1\| hypothetical protein t3153 [Salmonella enterica subsp. enterica serovar... gi\|213857436 - gi\|213857436\|ref\|ZP_03384407.1\| hypothetical protein SentesT_19959 [Salmonella enterica subsp. enter... gi\|213586182 - gi\|213586182\|ref\|ZP_03368008.1\| hypothetical protein SentesTyph_34913 [Salmonella enterica subsp. en... gi\|213161087 - gi\|213161087\|ref\|ZP_03346797.1\| hypothetical protein Salmoneentericaenterica_14003 [Salmonella enter...	0.0	[6..367]	[16..317]
lcl\|NC_016856.1_... - [gene=yqjG] [protein=putative glutathione S-transferase] [protein_id=YP_005239413.1] [location=34167... gi\|199605221, gi... - gi\|200388123\|ref\|ZP_03214735.1\| glutathione S-transferase [Salmonella enterica subsp. enterica serov... gi\|194450561, gi... - gi\|194450561\|ref\|YP_002047270.1\| glutathione S-transferase [Salmonella enterica subsp. enterica sero... gi\|197240221, gi... - gi\|197261966\|ref\|ZP_03162040.1\| glutathione S-transferase [Salmonella enterica subsp. enterica serov... gi\|205331193, gi... - gi\|205331193\|gb\|EDZ17957.1\| glutathione S-transferase [Salmonella enterica subsp. enterica serovar 4... gi\|205337878, gi... - gi\|205337878\|gb\|EDZ24642.1\| glutathione S-transferase [Salmonella enterica subsp. enterica serovar H... gi\|16766532, gi\|... - gi\|16766532\|ref\|NP_462147.1\| putative glutathione S-transferase [Salmonella typhimurium LT2], gi\|164...	0.0	[6..367]	[16..317]
gi\|254038274, gi... - gi\|254038274\|ref\|ZP_04872332.1\| conserved hypothetical protein [Escherichia sp. 1_1_43], gi\|22683989... YQJG_ECOLI - Hypothetical protein yqjG - Escherichia coli gi\|85675902, gi\|... - gi\|89109871\|ref\|AP_003651.1\| predicted S-transferase [Escherichia coli str. K-12 substr. W3110], gi\|... gi\|238902212, gi... - gi\|238902212\|ref\|YP_002928008.1\| putative S-transferase [Escherichia coli BW2952], gi\|238863110\|gb\|A... gi\|170082644, gi... - gi\|170082644\|ref\|YP_001731964.1\| S-transferase [Escherichia coli str. K-12 substr. DH10B], gi\|169890... EG12746 - putative S-transferase / Probable GSH transferase; disulfide-bond reductase EG12746 - putative S-transferase / Probable GSH transferase; disulfide-bond reductase	0.0	[6..367]	[16..317]
gi\|25346669 - pir\|\|AI3308 glutathione transferase (EC 2.5.1.18) [imported] - Brucella melitensis (strain 16M) gi\|17982364, gi\|... - gi\|17986738\|ref\|NP_539372.1\| glutathione S-transferase [Brucella melitensis 16M], gi\|17982364\|gb\|AAL...	0.0	[6..366]	[16..315]

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Predicted Domain #1

Region A:
Residues: [1-198]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSKQWASGTN GAFKRQVSSF RETISKQHPI YKPAKGRYWL YVSLACPWAH RTLITRALKG  60
   61 LTSVIGCSVV HWHLDEKGWR FLDMEKQLED SEDFLEHWHD VAGGIRTAKE DSSKSFAEIK 120
  121 NDSQRFMVDA TNEPHYGYKR ISDLYYKSDP QYSARFTVPV LWDLETQTIV NNESSEIIRI 180
  181 LNSSAFDEFV DDDHKKTD

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	14.99
Match:	1eemA
Description:	Class omega GST
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
glutathione binding	1.77211489459567	bayes_pls_golite062009
glutathione transferase activity	1.4787810404032	bayes_pls_golite062009
antioxidant activity	1.3961148524753	bayes_pls_golite062009
peroxidase activity	1.2460437903417	bayes_pls_golite062009
oxidoreductase activity, acting on peroxide as acceptor	1.2460437903417	bayes_pls_golite062009
binding	1.18507788580647	bayes_pls_golite062009
oxidoreductase activity, acting on sulfur group of donors	0.699008336089393	bayes_pls_golite062009
glutathione peroxidase activity	0.651680638869122	bayes_pls_golite062009
catalytic activity	0.646676412582618	bayes_pls_golite062009
protein binding	0.376412693567268	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [199-370]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LVPAQLKTQI DDFNSWVYDS INNGVYKTGF AEKAEVYESE VNNVFEHLDK VEKILSDKYS  60
   61 KLKAKYGEED RQKILGEFFT VGDQLTEADI RLYTTVIRFD PVYVQHFKCN FTSIRAGYPF 120
  121 IHLWVRNLYW NYDAFRYTTD FDHIKLHYTR SHTRINPLGI TPLGPKPDIR PL

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	14.99
Match:	1eemA
Description:	Class omega GST
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: