YRC Public Data Repository - Protein Structure Prediction

Protein:	MES1
Organism:	Saccharomyces cerevisiae
Length:	751 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MES1.

Description	E-value	Query Range	Subject Range
gi\|190406732 - gi\|190406732\|gb\|EDV09999.1\| methionine-tRNA ligase [Saccharomyces cerevisiae RM11-1a] MES1 - Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which in... SYMC_YEAST - Methionine--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MES...	0.0	[1..751]	[1..751]
SYMC_SCHPO - Probable methionine--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843)... SPBC17A3.04c - methionine-tRNA ligase	0.0	[20..748]	[19..778]
MARS - methionyl-tRNA synthetase HGNC:6898\|MIM:15... - synthetase gi\|60656267 - gi\|60656267\|gb\|AAX32697.1\| methionine-tRNA synthetase [synthetic construct]	0.0	[97..750]	[159..821]
SYM_METTH - Methionine--tRNA ligase OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JC... gi\|7437664 - pir\|\|G69177 methionine-tRNA ligase (EC 6.1.1.10) - Methanobacterium thermoautotrophicum (strain Delt...	0.0	[197..746]	[3..540]
SYMC_ARATH - Methionine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana GN=At4g13780 PE=2 SV=1	0.0	[189..750]	[10..587]
SYM_PYRAB - Methionyl-tRNA synthetase OS=Pyrococcus abyssi GN=metG PE=1 SV=1 SYM_PYRAB - Methionine--tRNA ligase OS=Pyrococcus abyssi (strain GE5 / Orsay) GN=metG PE=1 SV=1	0.0	[196..748]	[2..562]
SYM_GEOSE - Methionine--tRNA ligase OS=Geobacillus stearothermophilus GN=metG PE=3 SV=1	0.0	[194..750]	[2..528]

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Predicted Domain #1

Region A:
Residues: [1-190]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSFLISFDKS KKHPAHLQLA NNLKIALALE YASKNLKPEV DNDNAAMELR NTKEPFLLFD  60
   61 ANAILRYVMD DFEGQTSDKY QFALASLQNL LYHKELPQQH VEVLTNKAIE NYLVELKEPL 120
  121 TTTDLILFAN VYALNSSLVH SKFPELPSKV HNAVALAKKH VPRDSSSFKN IGAVKIQADL 180
  181 TVKPKDSEIL

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [191-211]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PKPNERNILI TSALPYVNNV P

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [234-316]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NYNALFICGT DEYGTATETK ALEEGVTPRQ LCDKYHKIHS DVYKWFQIGF DYFGRTTTDK  60
   61 QTEIAQHIFT KLNSNGYLEE QSM

[Run NCBI BLAST on this sequence.]

Region C:
Residues: [379-516]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SDHIFLSLDK LESQISEWVE KASEEGNWSK NSKTITQSWL KDGLKPRCIT RDLVWGTPVP  60
   61 LEKYKDKVLY VWFDATIGYV SITSNYTKEW KQWWNNPEHV SLYQFMGKDN VPFHTVVFPG 120
  121 SQLGTEENWT MLHHLNTT

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	777.0
Match:	1f4lA_
Description:	Methionyl-tRNA synthetase (MetRS); Methionyl-tRNA synthetase (MetRS), Zn-domain
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
ligase activity, forming aminoacyl-tRNA and related compounds	7.16276509815667	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	7.16276509815667	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	7.13957775963187	bayes_pls_golite062009
ligase activity	6.11211476973434	bayes_pls_golite062009
methionine-tRNA ligase activity	5.54691369460949	bayes_pls_golite062009
isoleucine-tRNA ligase activity	3.76808520367507	bayes_pls_golite062009
glutamate-tRNA ligase activity	2.63681738794687	bayes_pls_golite062009
catalytic activity	2.37674226834122	bayes_pls_golite062009
tyrosine-tRNA ligase activity	1.88984817882585	bayes_pls_golite062009
arginine-tRNA ligase activity	1.50161401062914	bayes_pls_golite062009
tryptophan-tRNA ligase activity	1.14187887833901	bayes_pls_golite062009
nucleotidyltransferase activity	1.06535308346284	bayes_pls_golite062009
cysteine-tRNA ligase activity	0.844943947059557	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.625793556381714	bayes_pls_golite062009
nucleic acid binding	0.449504767632354	bayes_pls_golite062009
glutamine-tRNA ligase activity	0.118292082006187	bayes_pls_golite062009
binding	0.090743795600025	bayes_pls_golite062009

Predicted Domain #3

Region A: Residues: [212-233]	1 11 21 31 41 51 \| \| \| \| \| \| 1 HLGNIIGSVL SADIFARYCK GR [Run NCBI BLAST on this sequence.]
Region B: Residues: [317-378]	1 11 21 31 41 51 \| \| \| \| \| \| 1 KQLYCPVHNS YLADRYVEGE CPKCHYDDAR GDQCDKCGAL LDPFELINPR CKLDDASPEP 60 61 KY [Run NCBI BLAST on this sequence.]
Region C: Residues: [517-574]	1 11 21 31 41 51 \| \| \| \| \| \| 1 EYLQYENGKF SKSRGVGVFG NNAQDSGISP SVWRYYLASV RPESSDSHFS WDDFVARN [Run NCBI BLAST on this sequence.]
Region D: Residues: [627-639]	1 11 21 31 41 51 \| \| \| \| \| \| 1 VKEMELGHER RGL [Run NCBI BLAST on this sequence.]
Region E: Residues: [733-751]	1 11 21 31 41 51 \| \| \| \| \| \| 1 RIDEKKIDEW RAKYGGQQV [Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	777.0
Match:	1f4lA_
Description:	Methionyl-tRNA synthetase (MetRS); Methionyl-tRNA synthetase (MetRS), Zn-domain
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [575-626]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NSELLANLGN FVNRLIKFVN AKYNGVVPKF DPKKVSNYDG LVKDINEILS NY

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [640-732]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EIAMSLSARG NQFLQENKLD NTLFSQSPEK SDAVVAVGLN IIYAVSSIIT PYMPEIGEKI  60
   61 NKMLNAPALK IDDRFHLAIL EGHNINKAEY LFQ

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	777.0
Match:	1f4lA_
Description:	Methionyl-tRNA synthetase (MetRS); Methionyl-tRNA synthetase (MetRS), Zn-domain
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: