YRC Public Data Repository - Protein Structure Prediction

Protein:	THI4
Organism:	Saccharomyces cerevisiae
Length:	326 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for THI4.

Description	E-value	Query Range	Subject Range
THI4 - Thiazole synthase, catalyzes formation of a thiazole intermediate during thiamine biosynthesis; requ... gi\|207345063 - gi\|207345063\|gb\|EDZ72005.1\| YGR144Wp-like protein [Saccharomyces cerevisiae AWRI1631] THI4_YEAST - Thiamine thiazole synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=THI4 PE=1 SV=... gi\|190406840 - gi\|190406840\|gb\|EDV10107.1\| hypothetical protein SCRG_00876 [Saccharomyces cerevisiae RM11-1a]	5.0E-70	[1..326]	[1..326]
gi\|6552395 - gi\|6552395\|dbj\|BAA88227.1\| thiamin biosynthetic enzyme [Glycine max]	5.0E-67	[5..324]	[22..316]
THI4_FUSOX - Thiamine thiazole synthase OS=Fusarium oxysporum GN=sti35 PE=1 SV=2 gi\|280494 - pir\|\|B37767 stress-inducible protein sti35 - fungus (Fusarium oxysporum) THI4_FUSO4 - Thiamine thiazole synthase OS=Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC...	1.0E-66	[2..324]	[14..311]
THI4_ARATH - Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana GN=THI1 PE=1 SV=1	5.0E-65	[3..324]	[21..318]
gi\|197241937, gi... - gi\|197263682\|ref\|ZP_03163756.1\| protein AegA [Salmonella enterica subsp. enterica serovar Saintpaul ... gi\|205329923, gi... - gi\|205329923\|gb\|EDZ16687.1\| protein AegA [Salmonella enterica subsp. enterica serovar 4,[5],12:i:- s... lcl\|NC_016856.1_... - [gene=aegA] [protein=putative oxidoreductase Fe-S binding subunit] [protein_id=YP_005238569.1] [loca... gi\|16421020, gi\|... - gi\|16765799\|ref\|NP_461414.1\| putative oxidoreductase Fe-S binding subunit [Salmonella typhimurium LT...	1.0E-63	[3..304]	[263..561]
THI4_ASPOR - (Q9UUZ9) Thiazole biosynthetic enzyme, mitochondrial precursor	1.0E-63	[32..324]	[42..318]
gi\|505330 - gi\|505330\|gb\|AAB46944.1\| Fe-S center and glutamate synthase (GltD) protein [Escherichia coli]	3.0E-63	[3..304]	[263..561]

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Predicted Domain #1

Region A:
Residues: [1-326]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSATSTATST SASQLHLNST PVTHCLSDIV KKEDWSDFKF APIRESTVSR AMTSRYFKDL  60
   61 DKFAVSDVII VGAGSSGLSA AYVIAKNRPD LKVCIIESSV APGGGSWLGG QLFSAMVMRK 120
  121 PAHLFLQELE IPYEDEGDYV VVKHAALFIS TVLSKVLQLP NVKLFNATCV EDLVTRPPTE 180
  181 KGEVTVAGVV TNWTLVTQAH GTQCCMDPNV IELAGYKNDG TRDLSQKHGV ILSTTGHDGP 240
  241 FGAFCAKRIV DIDQNQKLGG MKGLDMNHAE HDVVIHSGAY AGVDNMYFAG MEVAELDGLN 300
  301 RMGPTFGAMA LSGVHAAEQI LKHFAA

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	33.154902
Match:	1gt8A_
Description:	Dihydropyrimidine dehydrogenase, N-terminal domain; Dihydropyrimidine dehydrogenase, domain 4; Dihydropyrimidine dehydrogenase, domain 3; Dihydropyrimidine dehydrogenase, domain 2; Dihydropyrimidine dehydrogenase, C-terminal domain
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
oxidoreductase activity	2.37832337019921	bayes_pls_golite062009
catalytic activity	1.56773888754354	bayes_pls_golite062009
binding	1.27489023992977	bayes_pls_golite062009
oxygen-dependent protoporphyrinogen oxidase activity	0.73467824429984	bayes_pls_golite062009
protein binding	0.324315727651349	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: