YRC Public Data Repository - Protein Structure Prediction

Protein:	BUD16
Organism:	Saccharomyces cerevisiae
Length:	312 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for BUD16.

Description	E-value	Query Range	Subject Range
gi\|207346076 - gi\|207346076\|gb\|EDZ72684.1\| YEL029Cp-like protein [Saccharomyces cerevisiae AWRI1631] gi\|190405536 - gi\|190405536\|gb\|EDV08803.1\| bud site selection protein BUD16 [Saccharomyces cerevisiae RM11-1a] BUD16_YEAST - Putative pyridoxal kinase BUD16 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BUD16 PE... BUD16 - Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active for...	8.0E-64	[1..312]	[1..312]
CE28472 - status:Partially_confirmed UniProt:Q95R04 protein_id:AAK73885.1	3.0E-58	[3..300]	[44..325]
PDXK_SHEEP - Pyridoxal kinase OS=Ovis aries GN=PDXK PE=1 SV=1	3.0E-57	[3..304]	[6..307]
PDXK - pyridoxal (pyridoxine, vitamin B6) kinase	1.0E-56	[3..304]	[6..307]
PDXK_RAT - Pyridoxal kinase OS=Rattus norvegicus GN=Pdxk PE=1 SV=1	1.0E-56	[3..304]	[6..307]
PDXK_PIG - Pyridoxal kinase OS=Sus scrofa GN=PDXK PE=1 SV=1	3.0E-55	[3..304]	[16..317]
gi\|7294969, gi\|2... - gi\|7294969\|gb\|AAF50298.1\| CG4446-PA, isoform A [Drosophila melanogaster], gi\|24661479\|ref\|NP_648301....	3.0E-53	[1..310]	[9..304]
gi\|218705137, gi... - gi\|218705137\|ref\|YP_002412656.1\| pyridoxamine kinase [Escherichia coli UMN026], gi\|218432234\|emb\|CAR... gi\|254592653, gi... - gi\|254793253\|ref\|YP_003078090.1\| pyridoxal kinase 2/pyridoxine kinase [Escherichia coli O157:H7 str.... gi\|13361812, gi\|... - gi\|15831599\|ref\|NP_310372.1\| pyridoxamine kinase [Escherichia coli O157:H7 str. Sakai], gi\|13361812\|... gi\|194434304, gi... - gi\|83569259\|ref\|ZP_00920716.1\| COG2240: Pyridoxal/pyridoxine/pyridoxamine kinase [Shigella dysenteri... gi\|25326117, gi\|... - pir\|\|E85770 pyridoxal kinase 2 / pyridoxine kinase [imported] - Escherichia coli (strain O157:H7, su... gi\|15802050, gi\|... - gi\|15802050\|ref\|NP_288072.1\| pyridoxamine kinase [Escherichia coli O157:H7 EDL933], gi\|12515628\|gb\|A... PDXY_ECO57 - Pyridoxal kinase PdxY OS=Escherichia coli O157:H7 GN=pdxY PE=3 SV=3 gi\|218369781, gi... - gi\|218699795\|ref\|YP_002407424.1\| pyridoxamine kinase [Escherichia coli IAI39], gi\|218369781\|emb\|CAR1... gi\|195939026 - gi\|195939026\|ref\|ZP_03084408.1\| pyridoxamine kinase [Escherichia coli O157:H7 str. EC4024]	2.0E-52	[1..301]	[2..279]

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Predicted Domain #1

Region A:
Residues: [1-312]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPRLLATQSH VVHGYVGNKA ATFPLQCLGW DVDCCNSVQF SNHTGYGLDK VFGTITRETD  60
   61 LKELLSGLFD NFSQDYQALL SGYLPNKNSV RCMGTYYAKF KEANPEMIWL MDPVMGDEGQ 120
  121 LYVSEDVIPE YRKLALSPKQ LVDIITPNQF ELEILYGGEI KTKEHLKKAL KKLHQTIPVI 180
  181 IVTSCDCKMF DDKDFIYCVA SMEGKTPIVY RVPFIDSYFT GVGDLFSALL LDRVYKILSN 240
  241 PTTTLKFEDQ VNNVLNVIQK VLKITRSYAS GKMKAKMGSA LEMKEMELRL IESRDIYETI 300
  301 NIHQTDYIYA RL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	46.09691
Match:	1jxhA_
Description:	4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase (HMP-phosphate kinase, ThiD)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
kinase activity	3.96411195020495	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	3.67035256855428	bayes_pls_golite062009
transferase activity	3.30865420017814	bayes_pls_golite062009
lithium ion binding	3.00550221878163	bayes_pls_golite062009
sodium ion binding	2.93046843966773	bayes_pls_golite062009
pyridoxal kinase activity	2.62798458074822	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	2.56927916030784	bayes_pls_golite062009
phosphomethylpyrimidine kinase activity	2.12104666683074	bayes_pls_golite062009
pyridoxal binding	2.04719269680858	bayes_pls_golite062009
catalytic activity	1.74474587246986	bayes_pls_golite062009
binding	1.4216897826135	bayes_pls_golite062009
carbohydrate kinase activity	1.32862180479506	bayes_pls_golite062009
nucleic acid binding	1.23363144435362	bayes_pls_golite062009
transcription regulator activity	1.21529398967164	bayes_pls_golite062009
potassium ion binding	1.18018952731159	bayes_pls_golite062009
alkali metal ion binding	1.18018952731159	bayes_pls_golite062009
DNA binding	0.954497955488766	bayes_pls_golite062009
transcription factor activity	0.376784274163604	bayes_pls_golite062009
protein binding	0.117280046908929	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: