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View Structure Prediction Details

Protein: PUF6
Organism: Saccharomyces cerevisiae
Length: 656 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PUF6.

Description E-value Query
Range
Subject
Range
PUF6_YEAST - Pumilio homology domain family member 6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=...
PUF6 - Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, re...
0.0 [1..656] [1..656]
SPCP1E11.11 - Puf family RNA-binding protein
PUF6_SCHPO - Pumilio homology domain family member 6 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=pu...
0.0 [4..654] [19..642]
gi|13507676, gi|... - gi|13507676|ref|NP_109647.1| pumilio 1 [Mus musculus], gi|11991652|gb|AAG42319.1|AF321909_1 pumilio ...
0.0 [30..398] [828..1167]
PUM1 - pumilio homolog 1 (Drosophila)
0.0 [30..398] [827..1164]
gi|16209558 - gi|16209558|gb|AAL14121.1| pumilio-like protein 1 [Xenopus laevis]
0.0 [30..398] [331..670]

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Predicted Domain #1
Region A:
Residues: [1-157]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAPLTKKTNG KRSAKEVSHS EKKLAKKPRI SIDSSDEESE LSKKEDAVSS SSDDDDLDDL  60
   61 STSDSEAEEE ADELDISDDS EEHENENEEK EGKDKSEGGE NGNHTEQRKL LKERKMQRKS 120
  121 GTQVQQIKSV WERLRVKTPP LPKQIREKLS NEIWELS

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 131.67837
Match: 1ib2A_
Description: Pumilio 1
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
binding 2.43298635785702 bayes_pls_golite062009
protein binding 1.50566694420704 bayes_pls_golite062009
RNA binding 1.20550543134698 bayes_pls_golite062009
structural molecule activity 1.20255343083458 bayes_pls_golite062009
nucleic acid binding 0.169755106399802 bayes_pls_golite062009
cytoskeletal protein binding 0.0330689772947346 bayes_pls_golite062009

Predicted Domain #2
Region A:
Residues: [158-257]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KDCISDLVLK HDASRIVQTL VKYSSKDRRE QIVDALKGKF YVLATSAYGK YLLVKLLHYG  60
   61 SRSSRQTIIN ELHGSLRKLM RHREGAYVVE DLFVLYATHE 

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 131.67837
Match: 1ib2A_
Description: Pumilio 1
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
binding 2.32703786067369 bayes_pls_golite062009
protein binding 1.3800948965226 bayes_pls_golite062009
structural molecule activity 1.20216103967092 bayes_pls_golite062009
RNA binding 1.04913960175873 bayes_pls_golite062009
nucleic acid binding 0.118461769548431 bayes_pls_golite062009

Predicted Domain #3
Region A:
Residues: [258-444]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QRQQMIKEFW GSEYAVFRET HKDLTIEKVC ESSIEKRNII ARNLIGTITA SVEKGSTGFQ  60
   61 ILHAAMREYV KIANEKEISE MIELLHEQFA ELVHTPEGSD VACTLVARAN AKERKLILKA 120
  121 LKNHAEKLIK NEYGNIVFIT ILNCVDDTVL VFKTFSPTVK EHLQEFIIDK FGRRPWLYIL 180
  181 LGLDGKY

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 131.67837
Match: 1ib2A_
Description: Pumilio 1
Matching Structure (courtesy of the PDB):

Predicted Domain #4
Region A:
Residues: [445-656]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 FSPIVKNELL RYIELSKATS KKDPLQRRHE LLSKFAPMFL STISKDYSSI LTENLGCQFI  60
   61 AEVLINDELY AQLNEKDQEK YQQVLNNILT TFKGDITEEE HPIHRAFSTR LLKALIQGGK 120
  121 WNNKEKKVIP LKNVQGLGVP FAEKLYDEII DSSNLLEWIN NADSSFTIVA LYETLKDQKE 180
  181 GKPFLKDLRG VQSKITTDES NKGSQLLAKL LK

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.


YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle