YRC Public Data Repository - Protein Structure Prediction

Protein:	SAC7
Organism:	Saccharomyces cerevisiae
Length:	654 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for SAC7.

Description	E-value	Query Range	Subject Range
SAC7 - GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mut... SAC7_YEAST - GTPase-activating protein SAC7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SAC7 PE=1... gi\|190404673 - gi\|190404673\|gb\|EDV07940.1\| GTPase activating protein for RHO1 [Saccharomyces cerevisiae RM11-1a]	0.0	[1..654]	[1..654]
OPHN1_MOUSE - Oligophrenin-1 OS=Mus musculus GN=Ophn1 PE=1 SV=1	3.0E-67	[89..507]	[323..781]
gi\|168278407 - gi\|168278407\|dbj\|BAG11083.1\| oligophrenin 1 [synthetic construct] OPHN1 - oligophrenin 1	1.0E-66	[89..507]	[323..781]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	9.0E-62	[19..319]	[138..443]

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Predicted Domain #1

Region A:
Residues: [1-68]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPNNTLKQGS KIENVSPSKG HVPSFWKQFI NNPKSMSSEN ITVPRSPTSL SRNAQPTTLK  60
   61 RPPLSSRP

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted functions:

Term	Confidence	Notes
Rho GTPase activator activity	4.58620967349979	bayes_pls_golite062009
Rac GTPase activator activity	3.29568423512865	bayes_pls_golite062009
GTPase regulator activity	2.70614727793157	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	2.68579854252375	bayes_pls_golite062009
binding	2.15139804299723	bayes_pls_golite062009
guanyl-nucleotide exchange factor activity	2.06038764955701	bayes_pls_golite062009
enzyme regulator activity	1.9750017471254	bayes_pls_golite062009
small GTPase regulator activity	1.95738547986197	bayes_pls_golite062009
transcription regulator activity	1.40452907766862	bayes_pls_golite062009
protein binding	1.29100556128076	bayes_pls_golite062009
Ras GTPase activator activity	1.09070479183477	bayes_pls_golite062009
cytoskeletal protein binding	1.03886555863373	bayes_pls_golite062009
Ras guanyl-nucleotide exchange factor activity	0.958295643211986	bayes_pls_golite062009
nucleic acid binding	0.924382263852473	bayes_pls_golite062009
actin binding	0.834823471990077	bayes_pls_golite062009
DNA binding	0.748649183906122	bayes_pls_golite062009
GTPase activator activity	0.717730683557543	bayes_pls_golite062009
enzyme activator activity	0.298334474872045	bayes_pls_golite062009
catalytic activity	0.194464246602439	bayes_pls_golite062009
transcription factor activity	0.124467557925988	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [69-221]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 YSYNTPTKDR KSFSKSAKQN NNNNNANSGT SPHAEFKNYR DMFLSNRNGF TGRVFGVTLA  60
   61 ESLSVASAEV IVQSELVSFG RIPIVVAKCG AYLKANGLET SGIFRIAGNG KRVKALQYIF 120
  121 SSPPDYGTKF NDWETYTVHD VASLLRRYLN NLA

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	89.30103
Match:	1rgp__
Description:	p50 RhoGAP domain
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [222-324]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EPLIPLSLYE QFRNPLRSRP RILRHMLTHE VSHPNANKTN NVTVKSSRQN YNDDGANDGD  60
   61 IEKEDAKDDE EKRRRKIRHK RRLTRDIRAA IKEYEELFVT LSN

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	89.30103
Match:	1rgp__
Description:	p50 RhoGAP domain
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [325-434]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DTKQLTIYLL DLLSLFARQS QFNLMSGRNL AAIFQPSILS HPQHDMDPKE YELSRLVVEF  60
   61 LIEYSYKLLP HLLKLAKREQ QERLSTENKK NNGDKQKTDP IEIPKITSSD

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	10.82
Match:	1pbwA
Description:	p85 alpha subunit RhoGAP domain
Matching Structure (courtesy of the PDB):

Predicted Domain #5

Region A:
Residues: [435-654]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SPPIVSSNKN PPAIDNNNKL DHTTLSPIST SIPENSSDLQ TSKMLKPPKQ RRPHSKSFGS  60
   61 TPVPPDVIAS NKRRTSLFPW LHKPGILSDT GDNGDLTATE AEGDDYEEEN VDPYGQSPSS 120
  121 VHSGSLPKQH YLPIPRMNRS LSGNSTNSSF NTRPISMILT SGNDNSADQL ELLSNTHSNN 180
  181 ERSNALPLTE DDGDERNSRS RKRESWFQRL TSRSGSANRA

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: