YRC Public Data Repository - Protein Structure Prediction

Protein:	MET8
Organism:	Saccharomyces cerevisiae
Length:	274 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MET8.

Description	E-value	Query Range	Subject Range
MET8_YEAST - Siroheme biosynthesis protein MET8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MET8 ... MET8 - Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prostheti...	7.0E-81	[1..274]	[1..274]
gi\|208726275, gi... - gi\|208806474\|ref\|ZP_03248811.1\| siroheme synthase [Escherichia coli O157:H7 str. EC4206], gi\|2087262... CYSG_ECOSM - Siroheme synthase OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=cysG PE=3 SV=1 gi\|208739702, gi... - gi\|208819579\|ref\|ZP_03259899.1\| siroheme synthase [Escherichia coli O157:H7 str. EC4042], gi\|2087397... gi\|208733150, gi... - gi\|208811873\|ref\|ZP_03253202.1\| siroheme synthase [Escherichia coli O157:H7 str. EC4045], gi\|2087331... CYSG_ECO55 - Siroheme synthase OS=Escherichia coli (strain 55989 / EAEC) GN=cysG PE=3 SV=1 gi\|217320428, gi... - gi\|217324207\|ref\|ZP_03440291.1\| siroheme synthase [Escherichia coli O157:H7 str. TW14588], gi\|217320... gi\|193061862, gi... - gi\|75260342\|ref\|ZP_00731610.1\| COG0007: Uroporphyrinogen-III methylase [Escherichia coli E22], gi\|19... gi\|189369618, gi... - gi\|189369618\|gb\|EDU88034.1\| siroheme synthase [Escherichia coli O157:H7 str. EC4501], gi\|168759399\|r... gi\|85676672, gi\|... - gi\|89110641\|ref\|AP_004421.1\| fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase/... gi\|189359351, gi... - gi\|189359351\|gb\|EDU77770.1\| siroheme synthase [Escherichia coli O157:H7 str. EC4401], gi\|168753127\|r... gi\|187771618, gi... - gi\|187771618\|gb\|EDU35462.1\| siroheme synthase [Escherichia coli O157:H7 str. EC4196], gi\|168772734\|r... CYSG_ECOLC - Siroheme synthase OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=cysG PE=3 SV=1 gi\|168765722, gi... - gi\|189364751\|gb\|EDU83170.1\| siroheme synthase [Escherichia coli O157:H7 str. EC4486], gi\|168765722\|r... gi\|189374423, gi... - gi\|189374423\|gb\|EDU92839.1\| siroheme synthase [Escherichia coli O157:H7 str. EC869], gi\|168785179\|re... gi\|189002542, gi... - gi\|189002542\|gb\|EDU71528.1\| siroheme synthase [Escherichia coli O157:H7 str. EC4076], gi\|168779458\|r... CYSG_ECODH - Siroheme synthase OS=Escherichia coli (strain K12 / DH10B) GN=cysG PE=3 SV=1 gi\|168797146, gi... - gi\|189380160\|gb\|EDU98576.1\| siroheme synthase [Escherichia coli O157:H7 str. EC508], gi\|168797146\|re... gi\|194439502, gi... - gi\|83585121\|ref\|ZP_00923773.1\| COG0007: Uroporphyrinogen-III methylase [Escherichia coli 101-1], gi\|... CYSG_ECOHS - Siroheme synthase OS=Escherichia coli O9:H4 (strain HS) GN=cysG PE=3 SV=1 gi\|75211735, gi\|... - gi\|75211735\|ref\|ZP_00711799.1\| COG0007: Uroporphyrinogen-III methylase [Escherichia coli B171], gi\|1... gi\|191168412, gi... - gi\|75228409\|ref\|ZP_00715055.1\| COG0007: Uroporphyrinogen-III methylase [Escherichia coli B7A], gi\|19... CYSG_ECOBW - Siroheme synthase OS=Escherichia coli (strain K12 / MC4100 / BW2952) GN=cysG PE=3 SV=1 CYSG_ECO57 - Siroheme synthase OS=Escherichia coli O157:H7 GN=cysG PE=3 SV=1 gi\|242378894, gi... - gi\|251786621\|ref\|YP_003000925.1\| hypothetical protein B21_03171 [Escherichia coli BL21], gi\|24237889... gi\|253322849, gi... - gi\|254290046\|ref\|YP_003055794.1\| fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogen... gi\|12517996, gi\|... - gi\|15803881\|ref\|NP_289916.1\| siroheme synthase [Escherichia coli O157:H7 EDL933], gi\|12517996\|gb\|AAG... gi\|226839038, gi... - gi\|254038532\|ref\|ZP_04872588.1\| siroheme synthase [Escherichia sp. 1_1_43], gi\|226839038\|gb\|EEH71061... EG10188 - fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase /u... gi\|254163296, gi... - gi\|254163296\|ref\|YP_003046404.1\| fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogen... gi\|254594692, gi... - gi\|254795292\|ref\|YP_003080129.1\| fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogen... EG10188 - fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase /u... gi\|13363692, gi\|... - gi\|15833473\|ref\|NP_312246.1\| siroheme synthase [Escherichia coli O157:H7 str. Sakai], gi\|13363692\|db... CYSG_ECOLI - Siroheme synthase [Includes: Uroporphyrinogen-III C-methyltransferase (EC 2.1.1.107) (Urogen III met... gi\|168751811, gi... - gi\|188014206\|gb\|EDU52328.1\| siroheme synthase [Escherichia coli O157:H7 str. EC4113], gi\|168751811\|r...	3.0E-54	[2..254]	[1..210]
gi\|606302\|gb\|AAA... - CG Site No. 893 [Escherichia coli] gi\|606302 - gi\|606302\|gb\|AAA58165.1\| CG Site No. 893 [Escherichia coli]	1.0E-53	[2..254]	[1..210]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	5.0E-53	[2..254]	[1..210]
lcl\|NC_016856.1_... - [gene=cysG] [protein=siroheme synthase] [protein_id=YP_005239673.1] [location=3641327..3642700] gi\|205327600, gi... - gi\|205327600\|gb\|EDZ14364.1\| siroheme synthase [Salmonella enterica subsp. enterica serovar 4,[5],12:... gi\|197244145, gi... - gi\|197265890\|ref\|ZP_03165964.1\| siroheme synthase [Salmonella enterica subsp. enterica serovar Saint... CYSG_SALTY - Siroheme synthase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) GN=cysG PE=1 SV=1 CYSG_SALTY - Siroheme synthase OS=Salmonella typhimurium GN=cysG PE=1 SV=1 CYSG_SALTY - Siroheme synthase OS=Salmonella typhimurium GN=cysG PE=1 SV=1	5.0E-52	[2..254]	[1..210]
gi\|29144295, gi\|... - gi\|29144295\|ref\|NP_807637.1\| siroheme synthase [Salmonella enterica subsp. enterica serovar Typhi st... gi\|16762808 - gi\|16762808\|ref\|NP_458425.1\| siroheme synthase [Salmonella enterica subsp. enterica serovar Typhi st... gi\|213053371 - gi\|213053371\|ref\|ZP_03346249.1\| siroheme synthase [Salmonella enterica subsp. enterica serovar Typhi... gi\|213647200 - gi\|213647200\|ref\|ZP_03377253.1\| siroheme synthase [Salmonella enterica subsp. enterica serovar Typhi... CYSG_SALTI - Siroheme synthase OS=Salmonella typhi GN=cysG PE=3 SV=1 gi\|213613177 - gi\|213613177\|ref\|ZP_03371003.1\| siroheme synthase [Salmonella enterica subsp. enterica serovar Typhi... gi\|213426753 - gi\|213426753\|ref\|ZP_03359503.1\| siroheme synthase [Salmonella enterica subsp. enterica serovar Typhi... gi\|213416826 - gi\|213416826\|ref\|ZP_03349970.1\| siroheme synthase [Salmonella enterica subsp. enterica serovar Typhi... CYSG_SALTI - Siroheme synthase OS=Salmonella typhi GN=cysG PE=3 SV=1 gi\|214000213 - gi\|214000213\|ref\|ZP_03410948.1\| siroheme synthase [Salmonella enterica subsp. enterica serovar Typhi... gi\|213974705 - gi\|213974705\|ref\|ZP_03401964.1\| siroheme synthase [Salmonella enterica subsp. enterica serovar Typhi...	5.0E-52	[2..254]	[1..210]
CYSG2_KLEP7 - Siroheme synthase 2 OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) GN=c... gi\|238896851, gi... - gi\|238896851\|ref\|YP_002921596.1\| siroheme synthase [Klebsiella pneumoniae NTUH-K2044], gi\|238549178\|... gi\|11992137 - gi\|11992137\|gb\|AAG42457.1\|AF308467_3 siroheme synthase [Klebsiella aerogenes]	3.0E-51	[2..254]	[1..210]
CYSG2_YERPN - Siroheme synthase 2 OS=Yersinia pestis bv. Antiqua (strain Nepal516) GN=cysG2 PE=3 SV=1 gi\|161484726 - gi\|161484726\|ref\|NP_671234.2\| siroheme synthase [Yersinia pestis KIM] gi\|165928045, gi... - gi\|165928045\|ref\|ZP_02223877.1\| siroheme synthase [Yersinia pestis biovar Orientalis str. F1991016],... gi\|166010560, gi... - gi\|166010560\|ref\|ZP_02231458.1\| siroheme synthase [Yersinia pestis biovar Antiqua str. E1979001], gi... gi\|166213239, gi... - gi\|166213239\|ref\|ZP_02239274.1\| siroheme synthase [Yersinia pestis biovar Antiqua str. B42003004], g... gi\|167051152, gi... - gi\|167398648\|ref\|ZP_02304172.1\| siroheme synthase [Yersinia pestis biovar Antiqua str. UG05-0454], g... gi\|150260840, gi... - gi\|150260840\|ref\|ZP_01917568.1\| siroheme synthase [Yersinia pestis CA88-4125], gi\|149290248\|gb\|EDM40... gi\|167419358, gi... - gi\|167419358\|ref\|ZP_02311111.1\| siroheme synthase [Yersinia pestis biovar Orientalis str. MG05-1020]... gi\|165936152, gi... - gi\|89105793\|ref\|ZP_01178306.1\| COG0007: Uroporphyrinogen-III methylase [Yersinia pestis biovar Orien... gi\|167425680, gi... - gi\|167425680\|ref\|ZP_02317433.1\| siroheme synthase [Yersinia pestis biovar Mediaevalis str. K1973002]... CYSG_YERPA - Siroheme synthase OS=Yersinia pestis bv. Antiqua (strain Antiqua) GN=cysG PE=3 SV=1 gi\|167468236 - gi\|167468236\|ref\|ZP_02332940.1\| siroheme synthase [Yersinia pestis FV-1] gi\|45440022, gi\|... - gi\|45440022\|ref\|NP_991561.1\| siroheme synthase [Yersinia pestis biovar Microtus str. 91001], gi\|4543... CYSG_YERPE - Siroheme synthase OS=Yersinia pestis GN=cysG PE=3 SV=1 gi\|77634599, gi\|... - gi\|77634599\|ref\|ZP_00796696.1\| COG0007: Uroporphyrinogen-III methylase [Yersinia pestis Angola], gi\|... gi\|16120501, gi\|... - gi\|218927369\|ref\|YP_002345244.1\| siroheme synthase [Yersinia pestis CO92], gi\|16120501\|ref\|NP_403814...	4.0E-51	[2..252]	[1..205]
gi\|146315411, gi... - gi\|75826747\|ref\|ZP_00756182.1\| COG1648: Siroheme synthase (precorrin-2 oxidase/ferrochelatase domain... gi\|121629204, gi... - gi\|75815699\|ref\|ZP_00746195.1\| COG1648: Siroheme synthase (precorrin-2 oxidase/ferrochelatase domain... gi\|229608060, gi... - gi\|229608060\|ref\|YP_002878708.1\| siroheme synthase/precorrin-2 oxidase/sirohydrochlorin ferrochelata... gi\|229345107, gi... - gi\|229518392\|ref\|ZP_04407836.1\| siroheme synthase/precorrin-2 oxidase/sirohydrochlorin ferrochelatas... gi\|124119835, gi... - gi\|153221131\|ref\|ZP_01952166.1\| siroheme synthase component enzyme [Vibrio cholerae MAK 757], gi\|124... gi\|229505056, gi... - gi\|229505056\|ref\|ZP_04394566.1\| siroheme synthase/precorrin-2 oxidase/sirohydrochlorin ferrochelatas... gi\|153819354, gi... - gi\|153819354\|ref\|ZP_01972021.1\| siroheme synthase component enzyme [Vibrio cholerae NCTC 8457], gi\|1... gi\|227081534, gi... - gi\|227081534\|ref\|YP_002810085.1\| siroheme synthase component enzyme [Vibrio cholerae M66-2], gi\|2270... gi\|229511274, gi... - gi\|229511274\|ref\|ZP_04400753.1\| siroheme synthase/precorrin-2 oxidase/sirohydrochlorin ferrochelatas... gi\|15641375, gi\|... - gi\|9655857\|gb\|AAF94521.1\| siroheme synthase component enzyme [Vibrio cholerae O1 biovar El Tor str. ... gi\|11356164 - pir\|\|C82208 siroheme synthase component enzyme VC1363 [imported] - Vibrio cholerae (strain N16961 se... gi\|75830852, gi\|... - gi\|75830852\|ref\|ZP_00760123.1\| COG1648: Siroheme synthase (precorrin-2 oxidase/ferrochelatase domain...	1.0E-49	[2..244]	[1..201]

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Predicted Domain #1

Region A:
Residues: [1-179]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVKSLQLAHQ LKDKKILLIG GGEVGLTRLY KLIPTGCKLT LVSPDLHKSI IPKFGKFIQN  60
   61 EDQPDYREDA KRFINPNWDP TKNEIYEYIR SDFKDEYLDL EDENDAWYII MTCIPDHPES 120
  121 ARIYHLCKER FGKQQLVNVA DKPDLCDFYF GANLEIGDRL QILISTNGLS PRFGALVRD

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1608.221849
Match:	1kyqA_
Description:	Bifunctional dehydrogenase/ferrochelatase Met8p, N-terminal domain; Bifunctional dehydrogenase/ferrochelatase Met8p, dimerisation and C-terminal domains
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
transcription regulator activity	1.95832530533882	bayes_pls_golite062009
catalytic activity	1.79741889688267	bayes_pls_golite062009
DNA binding	1.70073234884672	bayes_pls_golite062009
binding	1.50616426395139	bayes_pls_golite062009
nucleic acid binding	1.47211855405194	bayes_pls_golite062009
transcription factor activity	0.9895904275677	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [180-274]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EIRNLFTQMG DLALEDAVVK LGELRRGIRL LAPDDKDVKY RMDWARRCTD LFGIQHCHNI  60
   61 DVKRLLDLFK VMFQEQNCSL QFPPRERLLS EYCSS

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1608.221849
Match:	1kyqA_
Description:	Bifunctional dehydrogenase/ferrochelatase Met8p, N-terminal domain; Bifunctional dehydrogenase/ferrochelatase Met8p, dimerisation and C-terminal domains
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: