YRC Public Data Repository - Protein Structure Prediction

Protein:	RFH_ECOLI
Organism:	Escherichia coli
Length:	166 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for RFH_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|56421641, gi\|... - gi\|56421641\|ref\|YP_148959.1\| peptide chain release factor 2 in translation [Geobacillus kaustophilus...	242.0	[0..2]	[163..126]
gi\|84689785, gi\|... - gi\|84704949\|ref\|ZP_01018449.1\| hypothetical protein PB2503_12974 [Parvularcula bermudensis HTCC2503]...	239.0	[0..1]	[164..109]
RF2_CAUCN - Peptide chain release factor 2 OS=Caulobacter crescentus (strain NA1000 / CB15N) GN=prfB PE=3 SV=1 RF2_CAUCR - Peptide chain release factor 2 OS=Caulobacter crescentus GN=prfB PE=3 SV=1 RF2_CAUCR - Peptide chain release factor 2 OS=Caulobacter crescentus (strain ATCC 19089 / CB15) GN=prfB PE=3 SV=...	238.0	[0..1]	[164..160]
gi\|134300908, gi... - gi\|88947567\|ref\|ZP_01150538.1\| Peptide chain release factor 2 [Desulfotomaculum reducens MI-1], gi\|8...	237.0	[0..1]	[163..125]
gi\|20807024, gi\|... - gi\|20807024\|ref\|NP_622195.1\| protein chain release factor B [Thermoanaerobacter tengcongensis MB4], ... gi\|20807024 - gi\|20807024\|ref\|NP_622195.1\| Protein chain release factor B [Thermoanaerobacter tengcongensis MB4]	237.0	[0..1]	[163..144]
gi\|39997376, gi\|... - gi\|39997376\|ref\|NP_953327.1\| peptide chain release factor 2 [Geobacter sulfurreducens PCA], gi\|39984...	237.0	[0..1]	[164..163]
gi\|110549044, gi... - gi\|110600263\|ref\|ZP_01388488.1\| peptide chain release factor 2 [Geobacter sp. FRC-32], gi\|110549044\|...	236.0	[0..1]	[164..155]
RF1_BORPA - Peptide chain release factor 1 OS=Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253... RF1_BORPA - Peptide chain release factor 1 OS=Bordetella parapertussis GN=prfA PE=3 SV=1	236.0	[0..2]	[166..148]
gi\|50876698, gi\|... - gi\|51245661\|ref\|YP_065545.1\| peptide chain release factor 2 [Desulfotalea psychrophila LSv54], gi\|50...	236.0	[0..1]	[163..84]
RF1_BORBR - Peptide chain release factor 1 OS=Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50... RF1_BORPE - Peptide chain release factor 1 OS=Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)... RF1_BORBR - Peptide chain release factor 1 OS=Bordetella bronchiseptica GN=prfA PE=3 SV=1 RF1_BORPE - Peptide chain release factor 1 OS=Bordetella pertussis GN=prfA PE=3 SV=1	236.0	[0..2]	[166..148]

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Predicted Domain #1

Region A:
Residues: [1-166]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLETETGRYS DTLRSALVSL DGDNAWALSE SWCGTIQWIC PSPYRPHHGR KNWFLGIGRF  60
   61 TADEQEQSDA IRYETLRSSG PGGQHVNKTD SAVRATHLAS GISVKVQSER SQHANKRLAR 120
  121 LLIAWKLEQQ QQENSAALKS QRRMFHHQIE RGNPRRTFTG MAFIEG

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	62.39794
Match:	1gqeA
Description:	Polypeptide chain release factor 2 (RF2)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
binding	1.72500611461832	bayes_pls_golite062009
nucleic acid binding	1.33510710518321	bayes_pls_golite062009
translation regulator activity	1.23360033083197	bayes_pls_golite062009
translation factor activity, nucleic acid binding	1.23313210840115	bayes_pls_golite062009
translation termination factor activity	1.224889505882	bayes_pls_golite062009
translation release factor activity	1.22486339070595	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: